Home | Community | Message Board

PlanetSpores.ca
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   Sporeworks.EU Spores for European Microscopy   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   Original Sensible Seeds Autoflowering Cannabis Seeds

Jump to first unread post Pages: | 1 | 2  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Re: Stalled wbs jars. What to do now? [Re: SHROOMSISAY01]
    #25507324 -

Oh my...:popcorn:
:tellmeastory:

Extras: Filter Print Post Top
Re: Stalled wbs jars. What to do now? [Re: Caps McGee]
    #25507663 -



Finally able to get the pictures uploading. I think I got the answers I needed, thank you everyone for the help!!!

Extras: Filter Print Post Top
Re: Stalled wbs jars. What to do now? [Re: SHROOMSISAY01]
    #25507917 -

Lmfao!


--------------------
“Silly Rabbi, Bacons for Gentiles!”

Extras: Filter Print Post Top
Re: Stalled wbs jars. What to do now? [Re: Trippies2122]
    #25507927 -



you got a lot of soggy wet grains in there. what grain prep are you using?



comparison of properly hydrated milo. millet is the smallest grain and has to be hydrated more carefully. fooman hot water bath worked well for me in preventing exploded grains when i used WBS mix.


--------------------
🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

Extras: Filter Print Post Top
Re: Stalled wbs jars. What to do now? [Re: JHOVA]
    #25507939 -

JHOVA said:


you got a lot of soggy wet grains in there. what grain prep are you using?



comparison of properly hydrated milo. millet is the smallest grain and has to be hydrated more carefully. fooman hot water bath worked well for me in preventing exploded grains when i used WBS mix.



I just soak millet for 24 hours drain for 15 mins throw them in a bag and pc for 3 hours perfect every time.

OP your jar looks like it has oatmeal in it not what you want. I think you found your problem. once done they should look very close to how they looked before you started. You want to just hydrate them not disintegrate them. See how his milo still looks like milo.

Edited by SHROOMSISAY01 (10/03/18 12:13 AM)

Extras: Filter Print Post Top
Re: Stalled wbs jars. What to do now? [Re: SHROOMSISAY01]
    #25508011 -

If I were you I would soak enough bird seed to do one jar for 24 hours drain it for 15 mins.  pressure cook for 90 mins. Then inoculate with your syringe. If you don't have a syringe left to inoculate with I would wrap a fork In aluminum foil and put it in your pc with the jar. Then I would use the fork to do a G2G transfer of just the good mycelium closet to the top of a jar. And if the Jar survives you can expand with a few more G2G transfers. I think what you have is the grain is to wet to be colonized but the mycelium is still good that grew in the jars. You really have nothing to loose trying this.

Extras: Filter Print Post Top
Re: Stalled wbs jars. What to do now? [Re: SHROOMSISAY01]
    #25508924 -

SHROOMSISAY01 said:
If I were you I would soak enough bird seed to do one jar for 24 hours drain it for 15 mins.  pressure cook for 90 mins. Then inoculate with your syringe. If you don't have a syringe left to inoculate with I would wrap a fork In aluminum foil and put it in your pc with the jar. Then I would use the fork to do a G2G transfer of just the good mycelium closet to the top of a jar. And if the Jar survives you can expand with a few more G2G transfers. I think what you have is the grain is to wet to be colonized but the mycelium is still good that grew in the jars. You really have nothing to loose trying this.



I am out of syringes but I just ordered more yesterday. But if that is a possibility with a good chance of the mycelium recovering I'll give it a shot rather than running through more syringes.

Extras: Filter Print Post Top
Re: Stalled wbs jars. What to do now? [Re: Trippies2122]
    #25509011 -

Trippies2122 said:


Finally able to get the pictures uploading. I think I got the answers I needed, thank you everyone for the help!!!



Is that some green in the bottom of jar three in your third picture, or a trick of the light?

Extras: Filter Print Post Top
Re: Stalled wbs jars. What to do now? [Re: trip_hazard]
    #25509065 -

If you used multiple syringes on those jars, you used way too much.  You dont need more than a few drops

Extras: Filter Print Post Top
Re: Stalled wbs jars. What to do now? [Re: Sivarted]
    #25509185 -

Sivarted said:
If you used multiple syringes on those jars, you used way too much.  You dont need more than a few drops



I am assuming he inoculated more than the jars in the pic. In his OP he said "So I have quite a few wbs jars that are coming up on being a month old" If not that is another problem I do 1 to 2 ml per jar when I used a syringe its been a while so you might want to check that. To much spore solution will change the water content of the jar and can make it to wet.

Extras: Filter Print Post Top
Re: Stalled wbs jars. What to do now? [Re: SHROOMSISAY01]
    #25509594 -

SHROOMSISAY01 said:
Sivarted said:
If you used multiple syringes on those jars, you used way too much.  You dont need more than a few drops



I am assuming he inoculated more than the jars in the pic. In his OP he said "So I have quite a few wbs jars that are coming up on being a month old" If not that is another problem I do 1 to 2 ml per jar when I used a syringe its been a while so you might want to check that. To much spore solution will change the water content of the jar and can make it to wet.



I assumed the same, but "several syringes" is too much for anything under like 50 or 60 jars (although I did assume he did fewer than that).  And using two spore prints for anything under like a couple hundred jars is massively wasteful.  (One print should make dozens of syringes)  2ml is probably too much, 1ml is unnecessary.  It really only takes a little bit, and anything beyond that is overkill (or, as you mentioned, actually harmful because of extra added water)

Of course, I also think using spores at all other than the very first time is less than ideal, so theres that.  :smile:

Extras: Filter Print Post Top
Re: Stalled wbs jars. What to do now? [Re: Sivarted]
    #25509631 -

I used half of 4 syringes, about 1-2 cc each jar. I said I used multiple syringes because someone suggested a bad syringe, I was just throwing out that I didnt use the same syringe for all the jars

Extras: Filter Print Post Top
Re: Stalled wbs jars. What to do now? [Re: Trippies2122]
    #25509637 -

Trippies2122 said:
I used half of 4 syringes, about 1-2 cc each jar. I said I used multiple syringes because someone suggested a bad syringe, I was just throwing out that I didnt use the same syringe for all the jars




It's already been pointed out that the most likely problem is your grain prep and them being too wet.

I was just pointing out that you dont need that much spore solution.  It really doesnt take that much, and more doesnt necessarily speed things up, either.

That's all.

Extras: Filter Print Post Top
Re: Stalled wbs jars. What to do now? [Re: Sivarted]
    #25509649 -

Also, definitely start working with agar, but dont bother trying to isolate.  Clone a mushroom and clean up the culture if necessary.

For 99.99999-ish percent of people in this hobby, isolation is both pointless and potentially harmful.  (What if what you end up isolating is a strain that fruits poorly, or not at all, for example)

Spores are the slowest and least clean way to inoculate jars.

Extras: Filter Print Post Top
Re: Stalled wbs jars. What to do now? [Re: Sivarted]
    #25509867 -

Sivarted said:
Also, definitely start working with agar, but dont bother trying to isolate.  Clone a mushroom and clean up the culture if necessary.

For 99.99999-ish percent of people in this hobby, isolation is both pointless and potentially harmful.  (What if what you end up isolating is a strain that fruits poorly, or not at all, for example)

Spores are the slowest and least clean way to inoculate jars.



Can you explain to me what you mean by clean up the culture? I thought isolating was cleaning it up? And making it into a single "cell" I know that's not right terminology. Isn't working with agar and isolating getting it to one true strain is what makes using agar so much faster and how people get jars colonized in 10 days and have such great canopies??

Extras: Filter Print Post Top
Re: Stalled wbs jars. What to do now? [Re: Trippies2122]
    #25513368 -

Trippies2122 said:
Sivarted said:
Also, definitely start working with agar, but dont bother trying to isolate.  Clone a mushroom and clean up the culture if necessary.

For 99.99999-ish percent of people in this hobby, isolation is both pointless and potentially harmful.  (What if what you end up isolating is a strain that fruits poorly, or not at all, for example)

Spores are the slowest and least clean way to inoculate jars.



Can you explain to me what you mean by clean up the culture? I thought isolating was cleaning it up? And making it into a single "cell" I know that's not right terminology. Isn't working with agar and isolating getting it to one true strain is what makes using agar so much faster and how people get jars colonized in 10 days and have such great canopies??



Not usually, no.  Working with agar is the best way to ensure contaminant free cultures, and starting from agar is significantly faster than spores.  Spore prints and syringes are rarely 100% clean, but if a culture on agar has any contams, they show up readily on the 2d agar surface.

When cloning, it's very easy to bring contamination in with the tissue, but also usually easy to transfer uncontaminated parts to a new plate and end up with a contaminant free culture.

You have a *lot* of reading to do before even considering trying to get an isolate, starting with why and how.  (And odds are very high that you will never have reason to)

And agar as well.  There are all sorts of intros to agar around here that explain both the hoes and the whys.

Search for some.

Extras: Filter Print Post Top
Re: Stalled wbs jars. What to do now? [Re: Sivarted]
    #25514085 -

"Cleaning up a culture" refers to obtaining a plate that contains ONLY the intended mycelium... I try to maintain as wide a genetic base as possible for initial tubs, then pick 5 or more fruit to clone for testing and further isolation

Extras: Filter Print Post Top
Jump to top Pages: | 1 | 2  [ show all ]

Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   Sporeworks.EU Spores for European Microscopy   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   Original Sensible Seeds Autoflowering Cannabis Seeds


Similar ThreadsPosterViewsRepliesLast post
* Innoculating a jar with multiple strains zanchin 4,389 9 03/07/02 01:02 PM
by zanchin
* Fridge temps to store inoculated WBS jar? Slit Sniff 554 6 10/05/18 05:12 PM
by Shroomspective
* Grain Transfer from the Masters mrshrums 4,781 4 02/23/02 05:26 PM
by Suntzu
* Multiple Use Lid
( 1 2 all )
SixTango 4,752 24 02/06/03 06:26 AM
by Anno
* G2G Transfer? lvleph 4,634 9 11/20/02 12:28 PM
by scifi5001
* substrate transfer?!?! dets 3,083 8 11/13/02 12:29 AM
by SixTango
* G2G transfers? lvleph 4,375 15 11/03/13 06:15 AM
by SpitballJedi
* Jar vs Bag Colonization Swami 2,120 4 02/28/02 05:20 PM
by SouthernGent

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
977 topic views. 9 members, 596 guests and 129 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.034 seconds spending 0.012 seconds on 19 queries.