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Offlinedets
higher mind andeducation
Registered: 11/12/02
Posts: 91
Last seen: 12 years, 1 month
substrate transfer?!?!
    #1047991 - 11/12/02 11:51 PM (14 years, 6 months ago)

first time i have every encountered this problem. I'm growing to cases of jars innoc. with p.ricans. I'm trying a new incubation method which involves almost a central air heating base. Only about 5 of the jars are almost fully colonized with amazing progress for one week. the other 19 are still moving at either a no growth or partial. Could i use substrate from the jars that have already been innoculated or should i just wait t make another syringe or something? what kind of risks do i run other than contamintion that i would have to worry about. i know puertos are a very vigiorous strain in its mycylic form, so i will be patient but i doesn't look to good. plz help. thanks.

dets



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"patriotism is the virtue of the vicious"


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OfflineSkikid16
fungus fan

Registered: 06/27/02
Posts: 5,666
Loc: In the middle of the nort...
Last seen: 12 years, 1 month
Re: substrate transfer?!?! [Re: dets]
    #1047995 - 11/12/02 11:54 PM (14 years, 6 months ago)

I don't get your question. Are you asking if you can transfer mycelium from the colonized jars to the uncolonized jars? If so, yes you can, but if the uncolonized jars have already been incoulated, I'd say you'd have more success by just waiting it out.


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Offlinedets
higher mind andeducation
Registered: 11/12/02
Posts: 91
Last seen: 12 years, 1 month
Re: substrate transfer?!?! [Re: Skikid16]
    #1048014 - 11/12/02 11:59 PM (14 years, 6 months ago)

yeah thats exactly what i was asking. This keyboard that i'm using isn't very good so some of my words are mispelt an i apoligize for it. The only reason i ask is because i have never had to do that before, and i'd rather be safe than sorry. oh well. 3 more weeks and i'll start a grow log. muushrooms wait for noone. heh.


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"patriotism is the virtue of the vicious"


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Invisiblematts
matts

Registered: 01/28/02
Posts: 3,649
[Re: dets]
    #1048034 - 11/13/02 12:08 AM (14 years, 6 months ago)



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OfflineSkikid16
fungus fan

Registered: 06/27/02
Posts: 5,666
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Re: substrate transfer?!?! [Re: matts]
    #1048054 - 11/13/02 12:19 AM (14 years, 6 months ago)

I agree that a flowhood would help, but it is not "definetly" needed. I did mycelium transfer with 6 jars, and all but one of them colonized without contams, and I don't have a flowhood.


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OfflineNew Name
New Name, OldUser
Registered: 11/11/02
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Loc: ATL
Last seen: 14 years, 5 months
Re: substrate transfer?!?! [Re: matts]
    #1048056 - 11/13/02 12:22 AM (14 years, 6 months ago)

The oven tek works well for this.


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Offlinedets
higher mind andeducation
Registered: 11/12/02
Posts: 91
Last seen: 12 years, 1 month
Re: substrate transfer?!?! [Re: New Name]
    #1048065 - 11/13/02 12:28 AM (14 years, 6 months ago)

thank alot for all your help. I'll make the transfer by the end of the week if i feel it will be neccessary.


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OfflineJovialLeprechaun
reasonableperson
Registered: 09/04/02
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Re: substrate transfer?!?! [Re: matts]
    #1048210 - 11/13/02 01:54 AM (14 years, 6 months ago)

I've done it many times using the oven tek and birdseed, works like a charm.....


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InvisibleSixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
Re: substrate transfer?!?! [Re: dets]
    #1048307 - 11/13/02 02:29 AM (14 years, 6 months ago)

IMHO, Oven tek is only for those that are lottery winner lucky. I am not that lucky, nor is my raccoon. A hot oven causes convetion of cool air from the floor to rise into the oven. Along with that unfiltered ait, comes possible contams.
  (c)ANNO

If you do not have a glove box or flow hood. Try this:

Grain to Grain Transfers (G2G)

Once you have learned to prepare sterilized spawn pint or quart jars of bird seed, rye or grains & fully colonize them with mycelium. You can easily propagate a single colonized quart jar of that into about 20 more via G2G transfers. Jars propagated via G2G transfers generally colonize under optimal conditions 100% in 10 to 14 days . Given that, spores do not have to germinate, as what is transferred to freshly prepared jars is active mycelium on fully colonized seed or grains.

The method is to prepare fresh jars, just as you would to inoculate via a spore syringe (soak seed, rinse, drain, load, apply filter disk & PC). Excepting, rather than inoculate the fresh jars with a syringe. You transfer grain from a colonized jar to fresh uncolonized jars. The procedure is simple & only requires common sense, minimal preparation, a long stout clean stainless steel spoon & the cleanest personal hygiene and the smallest uncarpeted working place you can muster.

Prepare the smallest cleanest uncarpeted room you have (generally, a bathroom). In the following manner. Clean the room as best you can, getting rid of any dirt, dust, mold or mildew. Remove any cloth hanging anywhere. Spray Lysol on everything, everywhere & wipe it down. If you have any hepa type air filter unit? Place it in the room & run it for at least 1 hour. Running a hepa is preferable, but, if you dont have one. You can usually manage without it

Wipe your fully colonized jar of grain & your fresh jars down, with a clean Lysol sprayed rag. Place those in the room, on the counter top, or whatever flat working surface you intend to use. Wear freshly laundered clean cloths. If you have a face mask  (preferable) wear it. If you have a shower cap to cover your hair (preferable), wear it. Enter the room, spray Lysol around (again) run the hepa for a few minutes (if you have one). Then, turn it off. Spray your hands & arms with Lysol & wipe dry.

Unscrew the lid off the colonized jar. Leave the internal filter disk or filter material in place covering the content. Unscrew the lid on a fresh jar, leaving the internal filter material in place. Remove the filter material from the colonized jar & dig up about ? of content, as it will be colonized into a solid mass. Spoon out 2 table spoons full & transfer them to the uncolonized jar, by lifting its filter up & spooning them in. Replace the filter material on the fresh jar IMMEDIATELY after spooning in the colonized material.

Repeat this same process as many times as you have fresh jars to transfer to. Once done. Screw the fresh jars lids on tight. Cover the outside of the lids of the fresh jars with a double layer of alcohol swabbed coffee filters & rubber band them down. Shake each fresh jar to spread the colonized material throughout it. Place your fresh jars in a dry, dark, warm place (preferably between 78 & 84 F), and allow them to colonize in peace & quiet. G2G transfer & shaking jars batters the mycelium. It takes it a day or 3  recuperate from that shock.  There is no need to shake  G2G jars more than once. As, doing so will only slow colonization, rather than speed it up.

Now, be careful, wise, live long, prosper & spread the magic. 6T  :tongue:


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Amazon Shop: ½ Pint Jars, HEPA Filter, Laminar Flow Hood, Rye Grain, Wild Bird Seed

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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