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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, HEPA Filter, Laminar Flow Hood, Rye Grain, Wild Bird Seed

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Offlinelvleph
mathematicalidiot

Registered: 11/12/02
Posts: 240
Last seen: 13 years, 6 months
G2G transfers?
    #1085865 - 11/25/02 11:52 PM (14 years, 4 months ago)

When doing a G2G could I just use a glove box or is it required to use a laminar flow hood?


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OfflineSkikid16
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Registered: 06/27/02
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Re: G2G transfers? [Re: lvleph]
    #1085873 - 11/25/02 11:55 PM (14 years, 4 months ago)

A glove box is good, a flowhood is better. I've done brf to brf transfers without either and 5 out of 6 jars were fine.


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OfflineNew Name
New Name, OldUser
Registered: 11/11/02
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Re: G2G transfers? [Re: lvleph]
    #1085888 - 11/25/02 11:59 PM (14 years, 4 months ago)

I just started using G2G transfers a few months ago and I just do them in the open air. Maybe I'm just lucky but so far I have done 15 quart jars of birdseed with absolutely no contamination.


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InvisibleSixTango
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Registered: 01/21/02
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Re: G2G transfers? [Re: lvleph]
    #1085974 - 11/26/02 12:20 AM (14 years, 4 months ago)

6T Grain to Grain Transfers (G2G)

Once you have learned to prepare sterilized spawn pint or quart jars of bird seed, rye or grains & fully colonize them with mycelium. You can easily propagate a single colonized quart jar of that into about 20 more via G2G transfers. Jars propagated via G2G transfers generally colonize under optimal conditions 100% in 10 to 14 days . Given that, spores do not have to germinate, as what is transferred to freshly prepared jars is active mycelium on fully colonized seed or grains.

The method is to prepare fresh jars, just as you would to inoculate via a spore syringe (soak seed, rinse, drain, load, apply filter disk & PC). Excepting, rather than inoculate the fresh jars with a syringe. You transfer grain from a colonized jar to fresh uncolonized jars. The procedure is simple & only requires common sense, minimal preparation, a long stout clean stainless steel spoon & the cleanest personal hygiene and the smallest uncarpeted working place you can muster.

Prepare the smallest cleanest uncarpeted room you have (generally, a bathroom). In the following manner. Clean the room as best you can, getting rid of any dirt, dust, mold or mildew. Remove any cloth hanging anywhere. Spray Lysol on everything, everywhere & wipe it down. If you have any hepa type air filter unit? Place it in the room & run it for at least 1 hour. Running a hepa is preferable, but, if you dont have one. You can usually manage without it

Wipe your fully colonized jar of grain & your fresh jars down, with a clean Lysol sprayed rag. Place those in the room, on the counter top, or whatever flat working surface you intend to use. Wear freshly laundered clean cloths. If you have a face mask  (preferable) wear it. If you have a shower cap to cover your hair (preferable), wear it. Enter the room, spray Lysol around (again) run the hepa for a few minutes (if you have one). Then, turn it off. Spray your hands & arms with Lysol & wipe dry.

Unscrew the lid off the colonized jar. Leave the internal filter disk or filter material in place covering the content. Unscrew the lid on a fresh jar, leaving the internal filter material in place. Remove the filter material from the colonized jar & dig up about ? of content, as it will be colonized into a solid mass. Spoon out 2 table spoons full & transfer them to the uncolonized jar, by lifting its filter up & spooning them in. Replace the filter material on the fresh jar IMMEDIATELY after spooning in the colonized material.

Repeat this same process as many times as you have fresh jars to transfer to. Once done. Screw the fresh jars lids on tight. Cover the outside of the lids of the fresh jars with a double layer of alcohol swabbed coffee filters & rubber band them down. Shake each fresh jar to spread the colonized material throughout it. Place your fresh jars in a dry, dark, warm place (preferably between 78 & 84 F), and allow them to colonize in peace & quiet. G2G transfer & shaking jars batters the mycelium. It takes it a day or 3  recuperate from that shock.  There is no need to shake G2G jars more than once. As, doing so will only slow colonization, rather than speed it up.
6T  :tongue:


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Offlinelvleph
mathematicalidiot

Registered: 11/12/02
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Re: G2G transfers? [Re: SixTango]
    #1087559 - 11/26/02 02:03 PM (14 years, 4 months ago)

Maybe, I am unlucky I did 24 in open air and none made it with out contam, so I decided to try using my glovebox I made.


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OfflineFred Garvin
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Re: G2G transfers? [Re: lvleph]
    #1087574 - 11/26/02 02:09 PM (14 years, 4 months ago)

I would guess that luck had nothing to do with your contam rate. Something obviously went very wrong. You should review your procedures and try to learn from and correct any mistakes. A glove box isn't normally neccessary, but it can't hurt, that's for sure.



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Offline420M
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Registered: 10/29/02
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Re: G2G transfers? [Re: Fred Garvin]
    #1098137 - 11/30/02 12:13 PM (14 years, 3 months ago)

Build a flowhood ONCE and forget it FOREVER!

~ 420M ~


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OfflineJMcDoogle
A Serious Scholar


Registered: 07/07/09
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Re: G2G transfers? [Re: 420M]
    #19077914 - 11/03/13 01:47 AM (3 years, 4 months ago)

A Flowhood is agreat investment, but maybe some people just
dont have the money to spend on it here and now. I seen a great
big true hepa filter on clearance at walmart today for 25 bucks originally something like 150 due to huge crack in it, im wondering
if the filter inside will still be viable?


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OfflineKizzle
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Re: G2G transfers? [Re: New Name]
    #19078375 - 11/03/13 02:48 AM (3 years, 4 months ago)

Quote:

New Name said:
I just started using G2G transfers a few months ago and I just do them in the open air. Maybe I'm just lucky but so far I have done 15 quart jars of birdseed with absolutely no contamination. 



And how many contaminated after spawning? That usually when contaminants appear after being introduced during a g2g. Ancient thread I know but still I hate hearing people say this.


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OfflineSupaThaRipper
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Re: G2G transfers? [Re: Kizzle]
    #19078392 - 11/03/13 02:53 AM (3 years, 4 months ago)

11 years guys... lol


Edited by SupaThaRipper (11/03/13 02:54 AM)


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OfflineKizzle
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Re: G2G transfers? [Re: SupaThaRipper]
    #19078405 - 11/03/13 02:56 AM (3 years, 4 months ago)

I wasn't the one who resurrected it though I'm just complaining :lol:


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OfflineJMcDoogle
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Registered: 07/07/09
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Re: G2G transfers? [Re: Kizzle]
    #19078415 - 11/03/13 03:01 AM (3 years, 4 months ago)

Oops, My bad -

Was at the bottom of the page, thought it was the one on the main page lol.


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OfflinecronicrM
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Re: G2G transfers? [Re: JMcDoogle]
    #19078429 - 11/03/13 03:06 AM (3 years, 4 months ago)

the ruff part is this question still pops up lol


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OfflineKizzle
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Re: G2G transfers? [Re: cronicr]
    #19078526 - 11/03/13 03:13 AM (3 years, 4 months ago)

It does pop up and this is some horribly outdated information. No TC these days would recommend open air g2gs using a spoon.:facepalm:


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OfflineJMcDoogle
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Re: G2G transfers? [Re: lvleph]
    #19078541 - 11/03/13 03:17 AM (3 years, 4 months ago)

Quote:

lvleph said:
Maybe, I am unlucky I did 24 in open air and none made it with out contam, so I decided to try using my glovebox I made.





:smbfacepalm:


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InvisibleSpitballJedi
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Re: G2G transfers? [Re: cronicr]
    #19078938 - 11/03/13 08:15 AM (3 years, 4 months ago)

Quote:

cronicr said:
the ruff part is this question still pops up lol




Sad but true


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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, HEPA Filter, Laminar Flow Hood, Rye Grain, Wild Bird Seed

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