Home | Community | Message Board


Mycohaus
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars, Brown Rice Flour, HEPA Filter, Laminar Flow Hood, Rye Grain, Wild Bird Seed

Jump to first unread post. Pages: 1
Offlinelvleph
mathematicalidiot

Registered: 11/12/02
Posts: 240
Last seen: 13 years, 3 months
G2G transfers?
    #1085865 - 11/25/02 11:52 PM (14 years, 13 days ago)

When doing a G2G could I just use a glove box or is it required to use a laminar flow hood?


--------------------
"Man was born free and is every where in chains."


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineSkikid16
fungus fan

Registered: 06/27/02
Posts: 5,666
Loc: In the middle of the nort...
Last seen: 11 years, 7 months
Re: G2G transfers? [Re: lvleph]
    #1085873 - 11/25/02 11:55 PM (14 years, 13 days ago)

A glove box is good, a flowhood is better. I've done brf to brf transfers without either and 5 out of 6 jars were fine.


--------------------
Re-Defeat Bush in '04


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineNew Name
New Name, OldUser
Registered: 11/11/02
Posts: 230
Loc: ATL
Last seen: 13 years, 11 months
Re: G2G transfers? [Re: lvleph]
    #1085888 - 11/25/02 11:59 PM (14 years, 13 days ago)

I just started using G2G transfers a few months ago and I just do them in the open air. Maybe I'm just lucky but so far I have done 15 quart jars of birdseed with absolutely no contamination.


--------------------
--------------------------------------------------------------------
"I will tell a cop that I know my fucking rights and we can match wits all night" -311
Don't let the cops search you. Let them know that you care about your rights.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleSixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
Re: G2G transfers? [Re: lvleph]
    #1085974 - 11/26/02 12:20 AM (14 years, 13 days ago)

6T Grain to Grain Transfers (G2G)

Once you have learned to prepare sterilized spawn pint or quart jars of bird seed, rye or grains & fully colonize them with mycelium. You can easily propagate a single colonized quart jar of that into about 20 more via G2G transfers. Jars propagated via G2G transfers generally colonize under optimal conditions 100% in 10 to 14 days . Given that, spores do not have to germinate, as what is transferred to freshly prepared jars is active mycelium on fully colonized seed or grains.

The method is to prepare fresh jars, just as you would to inoculate via a spore syringe (soak seed, rinse, drain, load, apply filter disk & PC). Excepting, rather than inoculate the fresh jars with a syringe. You transfer grain from a colonized jar to fresh uncolonized jars. The procedure is simple & only requires common sense, minimal preparation, a long stout clean stainless steel spoon & the cleanest personal hygiene and the smallest uncarpeted working place you can muster.

Prepare the smallest cleanest uncarpeted room you have (generally, a bathroom). In the following manner. Clean the room as best you can, getting rid of any dirt, dust, mold or mildew. Remove any cloth hanging anywhere. Spray Lysol on everything, everywhere & wipe it down. If you have any hepa type air filter unit? Place it in the room & run it for at least 1 hour. Running a hepa is preferable, but, if you dont have one. You can usually manage without it

Wipe your fully colonized jar of grain & your fresh jars down, with a clean Lysol sprayed rag. Place those in the room, on the counter top, or whatever flat working surface you intend to use. Wear freshly laundered clean cloths. If you have a face mask  (preferable) wear it. If you have a shower cap to cover your hair (preferable), wear it. Enter the room, spray Lysol around (again) run the hepa for a few minutes (if you have one). Then, turn it off. Spray your hands & arms with Lysol & wipe dry.

Unscrew the lid off the colonized jar. Leave the internal filter disk or filter material in place covering the content. Unscrew the lid on a fresh jar, leaving the internal filter material in place. Remove the filter material from the colonized jar & dig up about ? of content, as it will be colonized into a solid mass. Spoon out 2 table spoons full & transfer them to the uncolonized jar, by lifting its filter up & spooning them in. Replace the filter material on the fresh jar IMMEDIATELY after spooning in the colonized material.

Repeat this same process as many times as you have fresh jars to transfer to. Once done. Screw the fresh jars lids on tight. Cover the outside of the lids of the fresh jars with a double layer of alcohol swabbed coffee filters & rubber band them down. Shake each fresh jar to spread the colonized material throughout it. Place your fresh jars in a dry, dark, warm place (preferably between 78 & 84 F), and allow them to colonize in peace & quiet. G2G transfer & shaking jars batters the mycelium. It takes it a day or 3  recuperate from that shock.  There is no need to shake G2G jars more than once. As, doing so will only slow colonization, rather than speed it up.
6T  :tongue:


--------------------
~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinelvleph
mathematicalidiot

Registered: 11/12/02
Posts: 240
Last seen: 13 years, 3 months
Re: G2G transfers? [Re: SixTango]
    #1087559 - 11/26/02 02:03 PM (14 years, 12 days ago)

Maybe, I am unlucky I did 24 in open air and none made it with out contam, so I decided to try using my glovebox I made.


--------------------
"Man was born free and is every where in chains."


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineFred Garvin
Male Prostitute
Registered: 09/24/02
Posts: 1,657
Loc: The northern part of sout...
Last seen: 9 years, 10 months
Re: G2G transfers? [Re: lvleph]
    #1087574 - 11/26/02 02:09 PM (14 years, 12 days ago)

I would guess that luck had nothing to do with your contam rate. Something obviously went very wrong. You should review your procedures and try to learn from and correct any mistakes. A glove box isn't normally neccessary, but it can't hurt, that's for sure.



--------------------


The above statements are just the incoherent babblings of your friendly neighborhood Cracker!

Shur drinkin kils brane sells--but only the week ones!!


Post Extras: Print Post  Remind Me! Notify Moderator
Offline420M
Long Time Reader- First TimePoster

Registered: 10/29/02
Posts: 64
Last seen: 13 years, 9 months
Re: G2G transfers? [Re: Fred Garvin]
    #1098137 - 11/30/02 12:13 PM (14 years, 8 days ago)

Build a flowhood ONCE and forget it FOREVER!

~ 420M ~


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineJMcDoogle
A Serious Scholar


Registered: 07/07/09
Posts: 1,495
Loc: Nunavut
Last seen: 2 years, 4 months
Re: G2G transfers? [Re: 420M]
    #19077914 - 11/03/13 01:47 AM (3 years, 1 month ago)

A Flowhood is agreat investment, but maybe some people just
dont have the money to spend on it here and now. I seen a great
big true hepa filter on clearance at walmart today for 25 bucks originally something like 150 due to huge crack in it, im wondering
if the filter inside will still be viable?


--------------------
The ego is nothing other than the focus of conscious attention.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineKizzle
Misanthrope

Registered: 08/30/11
Posts: 8,641
Last seen: 1 day, 2 hours
Re: G2G transfers? [Re: New Name]
    #19078375 - 11/03/13 02:48 AM (3 years, 1 month ago)

Quote:

New Name said:
I just started using G2G transfers a few months ago and I just do them in the open air. Maybe I'm just lucky but so far I have done 15 quart jars of birdseed with absolutely no contamination. 



And how many contaminated after spawning? That usually when contaminants appear after being introduced during a g2g. Ancient thread I know but still I hate hearing people say this.


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineSupaThaRipper
DreamOfCultivation
 User Gallery

Registered: 09/02/13
Posts: 369
Loc: Northwest
Last seen: 8 months, 28 days
Re: G2G transfers? [Re: Kizzle]
    #19078392 - 11/03/13 02:53 AM (3 years, 1 month ago)

11 years guys... lol


Edited by SupaThaRipper (11/03/13 02:54 AM)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineKizzle
Misanthrope

Registered: 08/30/11
Posts: 8,641
Last seen: 1 day, 2 hours
Re: G2G transfers? [Re: SupaThaRipper]
    #19078405 - 11/03/13 02:56 AM (3 years, 1 month ago)

I wasn't the one who resurrected it though I'm just complaining :lol:


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineJMcDoogle
A Serious Scholar


Registered: 07/07/09
Posts: 1,495
Loc: Nunavut
Last seen: 2 years, 4 months
Re: G2G transfers? [Re: Kizzle]
    #19078415 - 11/03/13 03:01 AM (3 years, 1 month ago)

Oops, My bad -

Was at the bottom of the page, thought it was the one on the main page lol.


--------------------
The ego is nothing other than the focus of conscious attention.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflinecronicrM
Troll Eater
 User Gallery


Registered: 08/07/11
Posts: 51,625
Loc: Van Isle Flag
Last seen: 6 hours, 47 minutes
Trusted Cultivator
Re: G2G transfers? [Re: JMcDoogle]
    #19078429 - 11/03/13 03:06 AM (3 years, 1 month ago)

the ruff part is this question still pops up lol


--------------------
AMU Q N A




AMU Forum

"It doesn't matter what I think of you, all that matters is clean spawn"

Come chit-chat


THE BIG MELT


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineKizzle
Misanthrope

Registered: 08/30/11
Posts: 8,641
Last seen: 1 day, 2 hours
Re: G2G transfers? [Re: cronicr]
    #19078526 - 11/03/13 03:13 AM (3 years, 1 month ago)

It does pop up and this is some horribly outdated information. No TC these days would recommend open air g2gs using a spoon.:facepalm:


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineJMcDoogle
A Serious Scholar


Registered: 07/07/09
Posts: 1,495
Loc: Nunavut
Last seen: 2 years, 4 months
Re: G2G transfers? [Re: lvleph]
    #19078541 - 11/03/13 03:17 AM (3 years, 1 month ago)

Quote:

lvleph said:
Maybe, I am unlucky I did 24 in open air and none made it with out contam, so I decided to try using my glovebox I made.





:smbfacepalm:


--------------------
The ego is nothing other than the focus of conscious attention.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleSpitballJediS
Ancient Astronaut
Male User Gallery


Registered: 10/13/12
Posts: 8,567
Loc: Nibiru Flag
Trusted Cultivator
Re: G2G transfers? [Re: cronicr]
    #19078938 - 11/03/13 08:15 AM (3 years, 1 month ago)

Quote:

cronicr said:
the ruff part is this question still pops up lol




Sad but true


--------------------
The Basics
A little civility goes a long way

The Noob Forum
The Hammock Hangers' Forum


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: ½ Pint Jars, Brown Rice Flour, HEPA Filter, Laminar Flow Hood, Rye Grain, Wild Bird Seed

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* G2G transfers kjb1891 5,464 16 05/31/11 12:59 AM
by Javadog
* G2G Transfer? lvleph 1,799 9 11/20/02 02:28 PM
by scifi5001
* Shmuvbox: 9er Tek Cloning and G2g transfers
( 1 2 3 4 ... 15 16 all )
eatyualive 31,324 300 03/04/16 08:10 PM
by eatyualive
* G2G Transfer Device - ( No Flow Hoods or Glove Boxes Required)
( 1 2 all )
growguru 2,908 22 12/26/09 10:40 PM
by RogerRabbit
* G2G transfer problems idunno 855 11 07/18/10 01:02 PM
by Damion5050
* G2g transfers xwave3000 1,243 8 02/17/04 08:21 PM
by Deviant
* Has anyone experienced contamination problems with g2g transfers using a glove box?
( 1 2 all )
Big_Sexy 4,325 38 12/08/11 03:23 PM
by Big_Sexy
* G2G transfer help TrippinTime 962 18 12/29/10 12:01 PM
by RogerRabbit

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, Prisoner#1, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, EvilMushroom666, cronicr, PussyFart
1,746 topic views. 19 members, 54 guests and 30 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Azarius
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.052 seconds spending 0.003 seconds on 14 queries.