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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: The Biopsy Method: No Mercy for Bacteria. [Re: JHOVA]
    #24918749 -

will have to try this method out soon

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Re: The Biopsy Method: No Mercy for Bacteria. [Re: Josex]
    #24921384 -

Awesome tek!

Have done a needle biopsy on a good Ps. Cyan plate and inoculated 2 LC jars a few days ago through silicone self healing injection ports. :smile:

It was dilute beechwood tea + black tea with karo and a small bit of fertilizer (maybe should have added yeast nutrients instead) and pH adjusted to pH 8 or just under... one has a magnetic stirrer (the broken glass bits is new to me).. I also have canules specifically for being able to suck up blobby LC.

The stirrer makes a lot of noise in my slightly deformed filterlid containers - need a place where I can put it where it doesn't annoy me immensely.

Hope this works! Should I have used a more nutrient poor medium?


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    Re: The Biopsy Method: No Mercy for Bacteria. [Re: Solipsis] * 1
        #24935105 -

    Josex, you're tek saved my ape. I ran out of spores and only had very bacterial growth. So bacterial I was unsure if it was actually just mold...

    I took a biopsy 9 days ago, transfered from the brf puck 2 days ago and have healthy cubensis mycelium today.

    Nice job on this one man. So many of the best teks are commonsense that's gone by the wayside.


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    :ganja::mushroom::mushroom::flyhigh:
    :sporedrop:~~~:peyote::sanpedro:~~~:sporedrop:

    :chugbeer:

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    Re: The Biopsy Method: No Mercy for Bacteria. [Re: Changa Alchemist]
        #24935120 -

    brf is magic shit for us.:rofl:


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    πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

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    Re: The Biopsy Method: No Mercy for Bacteria. [Re: Changa Alchemist] * 1
        #24935127 -

    I'm happy to hear that man. :vibin:

    Yeah it's so common sense n simple that tbh Idk how the fuck this shit hasn't been tried before. I really don't get it. :shrug:


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    LC's are easy
    Go big with No-pours
    Clean yo shit
    Has u got Magic?

    PHENO HUNTERS

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    Re: The Biopsy Method: No Mercy for Bacteria. [Re: Josex]
        #24935140 -

    actually i was wondering why some older teks like 9er tek worked so well and now i get that brf is so badass it can suppress bacteria even from an alcohol lathered fork for transferring pf cakes doesnt stop it although eat now recommends a pced fork.


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    πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

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    Re: The Biopsy Method: No Mercy for Bacteria. [Re: Josex]
        #24935835 -

    Interesting technique.

    Do u use BRF pucks because BRF is naturally resistant to bacteria growth?


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    Re: The Biopsy Method: No Mercy for Bacteria. [Re: LogicaL Chaos]
        #24939163 -

    3 weeks and 2 bad LC’s later ill give this shit a shot. If it worked for van maybe it can work for me too.


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    πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

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    Re: The Biopsy Method: No Mercy for Bacteria. [Re: JHOVA]
        #24939172 -

    It'll work just do more than you think you should. It doesn't always take. I usually do 3 at a time. If I'm stressing on plates I'll do 2.


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    If I ever give out misinformation please inform me so I can have the correct information. :cheers:

    Tmethyl said:
    Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

    Caps McGee said:
    :thumbsup:
    Fun part is figuring out what works best for you

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    Re: The Biopsy Method: No Mercy for Bacteria. [Re: van hatton]
        #24939209 -

    hey van quick question? How many transfers from brf before you hit LC/grain etc?


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    πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

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    Re: The Biopsy Method: No Mercy for Bacteria. [Re: JHOVA]
        #24939995 -

    2 via pucks

    I tried one transfer and all the LCS went to shit.


    --------------------
    If I ever give out misinformation please inform me so I can have the correct information. :cheers:

    Tmethyl said:
    Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

    Caps McGee said:
    :thumbsup:
    Fun part is figuring out what works best for you

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    Re: The Biopsy Method: No Mercy for Bacteria. [Re: van hatton]
        #24940008 -

    Perfect.


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    πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

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    Re: The Biopsy Method: No Mercy for Bacteria. [Re: JHOVA]
        #24940044 -

    Thank you for this method. Was trying to find it to tell someone else about but forgot the name until today.

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    Re: The Biopsy Method: No Mercy for Bacteria. [Re: MooseShroom]
        #24940984 -

    Think I might try dropping a colonized grain on a puck. Got a bad jar and wanna try to save the myce.


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    Re: The Biopsy Method: No Mercy for Bacteria. [Re: Fascistbullyboy] * 1
        #24941000 -

    :randy:


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    Re: The Biopsy Method: No Mercy for Bacteria. [Re: 13shrooms]
        #24944433 -



    Clean spawn here i come mother fucker.


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    πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

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    Re: The Biopsy Method: No Mercy for Bacteria. [Re: JHOVA]
        #24944739 -

    Noice! :rockon:


    --------------------
    LC's are easy
    Go big with No-pours
    Clean yo shit
    Has u got Magic?

    PHENO HUNTERS

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    Re: The Biopsy Method: No Mercy for Bacteria. [Re: Josex]
        #24967022 -

    Dime sized culture. Im waiting until the culture becomes quarter sized to poke it for LC and test it on agar.




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    πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

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    Re: The Biopsy Method: No Mercy for Bacteria. [Re: JHOVA]
        #24989102 -

    I have a pinning coir puck innoc 21 days ago thats fast.
    Is there a possibility bacteria can be enmeshed within the pin body?
    Im plannin to clone this guy.


    1 out of 10 did pin, some stalled, some are fully colonized but with something piggy backing bothering the mycleium enough. Man my samples are bacterial AF.

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    Re: The Biopsy Method: No Mercy for Bacteria. [Re: pacmanbreed]
        #24989190 -

    @pacmanbreed
    The op states you can either biopsy again or preferably take a small radial portion to agar.

    As an update my culture is coming up fuckin clean on agar and just like you mentioned josex the growth is all over the place on the plate yet organized. I squirted a bit on agar; swirled it twice and dumped it on the floor of my sab. Surpisingly it was really fast showing growth in 2 days. Im prety excited to have clean spawn andnthanks for making this tek and sharing your knowledge.


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    πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

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    Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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