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OfflineFerather
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Tarragon Oyster T-Gel Wedges [Giveaway]
    #24934531 - 01/22/18 06:16 PM (2 years, 6 months ago)

I have [5x] T-Gel wedges of the rare Tarragon Oyster that I am willing to send out at the end of next month.
There will be more wedges soon, and I will update this thread when there is more to send.

Respond with: [X] number of wedges remaining, them PM me your details.

----
----


Tarragon Oyster (Pleurotus euosmus):


Tarragon Oyster Mushroom is a very rare oyster mushroom which is thought to be only found in the British Isles and is not cultivated commercially at all.


Mycelial Characteristics: White, longitudinally linear, cottony aerial fast growing and classically oysteresque. Soon after colonizing a petri dish of MYPA, the mycelium tears off in thick sheets.

Microscopic Features: This mushroom produces pale pinkish/lilac spores.

Suggested Agar Culture Media: Malt Yeast Agar (MYA), Malt Yeast Peptone Agar (MYPA), Potato Dextrose Yeast Peptone (PDYA), or Oatmeal Yeast Agar (OMYA).

Spawn Media: Grain spawn for the first two generations, hardwood sawdust spawn for the final stage.

Substrates for Fruiting: Hardwood sawdust, cereal straw, cottonseed hulls, sugar cane bagasse, coffee wastes, paper products, and many other materials. This mushroom will probably grow on many more substrate materials give modest experimentation.

Yield Potentials: 75-100% biological efficiency, greatly affected by the size of the mushrooms at harvest, and the number of flushes allowed.


---Growth Parameters---

Spawn Run:

Incubation Temperatures: 70-80° F (21-27° C)
Relative Humidity: 98-100%
Duration: 7-14 days
CO2: > 10,000 ppm
Fresh Air Exchanges: 0-1 per hour
Light Requirements: n/a


Primordia Formation:

Initiation Temperature: 65-75° F (18-24° C)
Relative Humidity: 95-100%
Duration: 7-10 days
CO2: < 2000 ppm
Fresh Air Exchanges: 4-8 per hour
Light Requirements: 750-1500 lux


Fruitbody Development:

Temperature: 70-80° F (21-27° C)
Relative Humidity: 90-95%
Duration: 4-8 days
CO2: < 1000 ppm
Fresh Air Exchanges: 4-8 per hour
Light Requirements: 750-1500 lux


Cropping Cycle:

3 crops, two weeks apart.


Comments: With a distinctive and delicate trumpet shaped cap, as it’s name suggests, it’s smell and taste resembles the herb tarragon.


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Edited by Ferather (01/25/18 06:59 AM)


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Offlinetscott1632
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #24934544 - 01/22/18 06:22 PM (2 years, 6 months ago)

4 wedges remaining.
Thanks


--------------------
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Offlinemyceliups
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: tscott1632]
    #24934556 - 01/22/18 06:24 PM (2 years, 6 months ago)

3 remaining.. Thank you


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Offlinelewp
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: myceliups]
    #24934565 - 01/22/18 06:27 PM (2 years, 6 months ago)

2 remaining


--------------------
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OfflineXerbia
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: lewp]
    #24934575 - 01/22/18 06:31 PM (2 years, 6 months ago)

1 remaining


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OfflineMahdi
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Xerbia]
    #24934646 - 01/22/18 06:53 PM (2 years, 6 months ago)

All gone :smile:


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OfflineFerather
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Mahdi]
    #24934680 - 01/22/18 07:03 PM (2 years, 6 months ago)

Wow, no problem guys, I had better do more in the next run (low on vials).

I will PM all of you when the items have been dispatched.
Which should be around the 23rd of Feb.

I will also do more wedges on-around that date.


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OfflineXerbia
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather] * 1
    #24934701 - 01/22/18 07:11 PM (2 years, 6 months ago)

Quote:

Ferather said:
Wow, no problem guys, I had better do more in the next run (low on vials).

I will PM all of you when the items have been dispatched.
Which should be around the 23rd of Feb.

I will also do more wedges on-around that date.




Thanks man really appreciate the generosity! Can't wait to get some goin


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OfflineFerather
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Xerbia]
    #24936245 - 01/23/18 01:10 PM (2 years, 6 months ago)

:thumbup:


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OfflineFerather
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #24939433 - 01/24/18 06:07 PM (2 years, 6 months ago)

Extra data for Tarragon oyster:

Active enzymes (general): Cellulase (cellulose), Laccase (phenol's), Amylase (starch), Protease (protein) | Sugars (such as Sucrase).

Carbon sources (general): Cellulose, Starches, Sugars, Proteins, Phenol's, Other.
Nitrogen sources (general): Protein, Some Phenol's, Ammoniacal, Ureic, Other.

----

Tip: Tarragon doesn't like too much acidity, or too much phenol's.

Tested to work at pH 6.0-8.0, I've not tried below 6.0.

The optimal pH with paper was 7-7.5.


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OfflineTravelAgency
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather] * 2
    #24955785 - 01/30/18 09:22 PM (2 years, 6 months ago)

Results of Pine v Hardwood Pellets on Tarragon Oyster

Pine took an early lead and stayed that way, getting to 100% 6 days before HWFP, and has already pinned by the time I put the Hardwood in the FC, and also colonized much thicker. Yields were also in favor of the Pine by several ounces.

Colonization differences:



Pine:








HWFP:





Recipe was 60 cups pellets, 20 cups wheat bran, 5 gallons water for both. Harvest on Pine was a full week ahead of Hardwood.

Conclusion: Patterson’s Horse Bedding Pine Pellets colonize faster and produce Higher yields than do HWFP from Tractor Supply.

Caveat: I have used better quality HWFP in the past- there are a lot of small chunks that don’t turn into pure sawdust which may be a factor.

Observations: Tarragon is very stemmy, I had Golds and Bratslavian growing along with it that were far less stemmy than the Tarragon. It is also a beautiful strain, a grayish purple with dark purple stripes on the stipe and a speckled lighter purple for the caps. Haven’t cooked with them yet so I can’t attest to taste yet.


--------------------





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OfflineXerbia
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: TravelAgency]
    #24955898 - 01/30/18 10:11 PM (2 years, 6 months ago)

Awesome! Can't wait to cultivate these. Thanks for the comparison of substrate as well!


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OfflineFerather
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Xerbia]
    #24956380 - 01/31/18 06:30 AM (2 years, 6 months ago)

That's some nice BE you got there, not sure what's causing the wavy caps.
Excellent write up and description, are you collecting spores?

You can clone new fruiting genetics that suit more.
The vendor cloned a horizontal fruit.

:takingnotes:


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OfflineSolipsis
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #24962088 - 02/02/18 02:38 PM (2 years, 5 months ago)

I can share agar of this if it's so popular, in a thread i will make for that, as soon as I confirm that the discoloration and slight slickness I see are just related to laccase on T-gel and not bacteria or anything like that. You may need to reimburse shipping though if it's gonna be a lot of wedges cause I have no income... don't worry mail is quite affordable here relatively to certain countries (still it adds up)

Excited about this!!

Thank you for being generous and also much to Travel for comparing pine! Very helpful as I am looking into hardwood vs pine for any edibles.

Is this species like Hericium in that FAE / CO2 level is supposed to be a serious factor in how strong the aroma / taste is?

How might one get too much polyphenols in the substrate?


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OfflineFerather
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Solipsis] * 1
    #24962156 - 02/02/18 03:08 PM (2 years, 5 months ago)

It should be laccase enzymes (brown-tan). Currently I am adding tea, which adds acidic phenol's.
Adding too much tea slowed down the mycelium, wood can also be too rich.


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Offlinepacmanbreed
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25013494 - 02/22/18 02:56 PM (2 years, 5 months ago)

:bow2: how i wish i was close to your location.


--------------------

Bible, INSTRUCTION


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OfflineFerather
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: pacmanbreed]
    #25019044 - 02/24/18 05:06 PM (2 years, 5 months ago)

I will be sending the wedges Monday, sorry for the delay.


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather] * 1
    #25019061 - 02/24/18 05:16 PM (2 years, 5 months ago)

Thanks. I'm pretty excited. I'm gonna spend most
of my time growing this species along with the
shaggy mane.


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OfflineFerather
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: myceliups] * 1
    #25020480 - 02/25/18 10:35 AM (2 years, 5 months ago)

I have assembled the wedges into vials, and sealed the vials in zip-lock bags.
You are all getting two wedges in separate vials, one in each.


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25021640 - 02/25/18 06:50 PM (2 years, 5 months ago)

:popcorn:

Would love a chance at a wedge.

Nice stuff


--------------------





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OfflineFerather
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: FishLevelMidnight] * 1
    #25022927 - 02/26/18 09:45 AM (2 years, 5 months ago)

All wedges have been sent, I will do more wedges soon.


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Offlinechobumms
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25025455 - 02/27/18 04:05 AM (2 years, 5 months ago)

Quote:

Ferather said:
All wedges have been sent, I will do more wedges soon.




I'm looking forward to it!


--------------------
[/url]
My "Faster Germination For Popcorn Substrate" TEK
http://www.shroomery.org/forums/showflat.php/Number/8428361#8428361


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InvisibleHumble Newcomer
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: chobumms]
    #25025868 - 02/27/18 09:26 AM (2 years, 5 months ago)

Are you mailing to the states Ferather? If so I'd like to get on this. I could reply in kind and send you one of my new acquisitions that will be ready in the coming weeks, maybe when you have a few more ready.


--------------------
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OfflineFishLevelMidnightS
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Humble Newcomer]
    #25025871 - 02/27/18 09:27 AM (2 years, 5 months ago)

If anyone is willing to spread the love and send a sample of the tarragon oyster after they grow it out let me know! I have some thing for trade.

Thanks for everything you do Fer


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OfflineFerather
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: FishLevelMidnight]
    #25025908 - 02/27/18 09:47 AM (2 years, 5 months ago)

I will do more wedges soon on the updated T-Gel, I am using the microwave assembly, and adding my additives after the cook.
Currently I have 3 plates made on different days, a visual comparison is being made, on population and health.

Microwaving the pre-extract causes it oxidize, in a similar fashion to laccase enzymes.
The conversion also causes to agar to set very hard, without any sugars.

----

Control: Plain, no additives.

Recipe 1: Added 2g sucrose and 0.1g MG, per 100g extract.
Recipe 2: Added 2g 240B gelatin, per 100g extract.

----

Previous tests, using the old assembly (soft, not fully hardened).


Plain, no additives:

   


With added macro-micro nutrients (MG):

   


With added 240B (bloom) gelatin:

   


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: FishLevelMidnight]
    #25027038 - 02/27/18 06:35 PM (2 years, 5 months ago)

Pumped


Edited by tscott1632 (02/27/18 06:36 PM)


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OfflineFerather
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: tscott1632]
    #25028715 - 02/28/18 01:00 PM (2 years, 5 months ago)

T-Gel Recipe 1 has germinated and grown out a random mycelium after only 4.5 days.
Recipe 2 is yet to contaminate and is producing growth equal to Recipe 1.

Bottom: Control, Middle: Recipe 1, Top: Recipe 2.



----

It's lignicolous mold, there is a green-blue patch appearing. It's also penetrative (see below, right side - internal white).

----

Surface growth is more populated and aerial with Recipe 1 and Recipe 2, very similar results.
The both samples have penetrative growth, through the wedge and the main agar.

Recipe 1, is 2.5 days ahead of Recipe 2 in terms of sample assembly.

       

Sorry for the quality, not the best containers for images.


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Edited by Ferather (02/28/18 03:17 PM)


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OfflineFerather
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25029428 - 02/28/18 03:59 PM (2 years, 5 months ago)

Some data and notes for cultivators:

The T-Gel plate with added MG, but no sucrose, did not contaminate from open air, while with added sucrose did, rapidly.
Gelatin (change to 100g) is about 85-95% proteins, nutritional proteins count both as carbon and nitrogen.

Recipe 1, is producing equal results to Recipe 2 due to the addition of the sucrose as carbon.
In short the mixture of both carbon and nitrogen acts like adding proteins, in a way.

----

Tarragon oyster will also utilize other carbon sources with MG (WL-Tek).
It decay's the cellulose as carbon (no sugar, no starch).

   

Use clean spawn, wait 2-3 weeks.


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25029497 - 02/28/18 04:30 PM (2 years, 5 months ago)

Also, I forgot to mention Tarragon oyster will also utilize lime (CaCO3) as a carbon source, when soluble.


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InvisibleHumble Newcomer
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25030279 - 02/28/18 10:15 PM (2 years, 5 months ago)

How long was it exposed to open air? That's cool


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Humble Newcomer]
    #25030915 - 03/01/18 05:12 AM (2 years, 5 months ago)

These 3 plates where poured open to air, and set open to air, that's about 2 hours.

The lignicolous mold is very healthy, very populated and well fed.
Cubensis also germinates on sucrose + MG, in 4 days.



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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25031025 - 03/01/18 06:52 AM (2 years, 5 months ago)

So t gel made with MG and no added sucrose, was poured hot in a dish in open air, allowed to set in open air approximately 2 hours later, and to this date it still hasnt germinated any airborne spores/bacteria/yeast?

How long ago did you pour? The other plate with sucrose that contaminated quickly, it was poured at the same time?

:eek:


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Humble Newcomer]
    #25032870 - 03/02/18 07:02 AM (2 years, 5 months ago)

Not quite that much of a test, I am testing open to air assembly, with the push of setting the agar open to air.
All samples are inoculated with an oyster sample after fully setting, general data is recorded.

I am testing effects on growth, and chance to contaminate from assembly-other.
So far Recipe 2 is slower than Recipe 1, Recipe 1 has only mold.

All 3 samples are clear of visible bacteria-yeast.


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25033305 - 03/02/18 10:43 AM (2 years, 5 months ago)

Recipe 1: Added 2g sucrose and 0.1g MG, per 100g extract.
Recipe 2: Added 2g 240B gelatin, per 100g extract.

Recipe 3: Added 1.6g of sucrose, per 100g extract.
Recipe 4: Added 0.1g MG, per 100g extract.

----

Control:

Plain T-Gel, no additives. Normal speed and population rate for T-Gel, no contamination.


Current results:

Recipe 1: Produced better growth, mold after 4.5 days, no bacteria-yeast.
Recipe 2: Negative impact to speed, no contamination.


Previous results:

Recipe 3: A tiny bit better in general growth, no contamination.
Recipe 4: Improved population, no contamination.


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25033341 - 03/02/18 10:57 AM (2 years, 5 months ago)

Some people have received their wedges already, but they are European or close.
I realize I have not labelled the sample wedges, and isolate name.

Pleurotus euosmus (Tarragon Oyster): TAR01

----

I've ditched the moldy Recipe 1, and will be trying Recipe 5:

Recipe 5: Added 0.2g MG, per 100g extract.


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Edited by Ferather (03/02/18 12:41 PM)


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25037013 - 03/03/18 09:31 PM (2 years, 5 months ago)

Very interesting. Your oyster pics are beautiful


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: chobumms]
    #25037558 - 03/04/18 06:13 AM (2 years, 4 months ago)

Thanks, Golden oyster loves: Sunlight + water (rain) + high humidity + high O2.
Tarragon oyster needs far less light, but it produces thicker stems.

Tarragon oyster will also fruit very well horizontally.

----

Parent:

Pleurotus cornucopiae (Branched Oyster).

Variations:

Pleurotus citrinopileatus (Golden Oyster).
Pleurotus euosmus (Tarragon Oyster).


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25037795 - 03/04/18 09:17 AM (2 years, 4 months ago)

Recipe 5: Added 0.2g MG, per 100g extract:

Improved laccase production (more browning), I believe this is due to two reasons, enzymes are proteins, they are composed of C-H-N-O.
Laccase also contains copper, so it's C-H-N-O-Cu, the MG adds nitrogen and copper to the T-Gel media, which contains C-H-O.

In general laccase will produce C-H-O from phenol's, and the N-Cu from the added MG, more enzymes = more C-H-O.

Recipe 2, 4 and 5 produced an increase to population rate, thickness and overall growth potential.
The slowest of the three is Recipe 2, nutritional proteins provide C-H-O and N-Other.

----

Recipe 1: Added 2g sucrose and 0.1g MG, per 100g extract.
Recipe 2: Added 2g 240B gelatin, per 100g extract.

Recipe 3: Added 1.6g of sucrose, per 100g extract.
Recipe 4: Added 0.1g MG, per 100g extract.


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25037833 - 03/04/18 09:35 AM (2 years, 4 months ago)

All of my samples produced less carbon based waste when the level of N-Other increased.

----

Next Test:

Recipe 6: Added 2g gelatin and 0.2g MG, per 100g extract.


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Edited by Ferather (03/04/18 03:35 PM)


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25044507 - 03/07/18 04:11 AM (2 years, 4 months ago)

I don't have malt extract so tweaked the T-gel recipe/concept a little before (and have in the process also created agar on which nothing seems to grow, lol), got a good one now though:

Around 150 ml water, simmer in it about 5-6g oats and a dash of bran, extract a teabag of black organic tea in it and strain carefully into erlenmeyer with 4(+) g agar powder suspended in about 50 ml cold / RT water, to make 200 ml agar.

I was already dying my medium for contrast - now that I have a few more colors I can color code for different media... although T-gel does this spontaneously by being brown. :wink:

I suspect that maltodextrine may be a suitable replacement for the oats or malt extract but not sure (I think oligosaccharides are mostly the point of the malt), but if I were to purchase new ingredients might as well go for the malt extract. Using yeast nutrition can provide micro-nutrients - mostly minerals and some other inorganics etc - bran surely has a richer organic micro-nutrient mix like including protein.

I was having a lot of tomentose growth on my PDA, and while growth wasn't necessarily slow it tended to be wispy initially (later consolidated), I believe I was allowing too much potato starch to be dissolved / suspended.

On the tweaked T-gel i mentioned I now am getting much more beautiful full white growth (that is, with Ovoids, subs, and now also gyms and Ps Mex though I have no idea how lignicolous it is as nobody grows truffles on sawdust lol) and it should work for gourmet woodlovers as well.

Might have to check differences between MG, bran and yeast nutrient (nutrivit)..

Growing Tarragon horizontally as in from trays?

By the way: while testing open air conditions might be interesting in *some* ways it seems like a bad plan because contaminants can still hitch a ride secretly even if they are not encouraged to grow and become dominant. Would you not expect problems once you go to spawn and bulk? Or are you just experimenting to see more acute sensitivity to seriously contaminate?
Just saying: you might wanna point out that you don't recommend open air conditions at this stage even with actual T-gel use if it is not meant for that.


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Solipsis]
    #25044609 - 03/07/18 06:22 AM (2 years, 4 months ago)

I'm glad you found a working additive for T-Gel, I am still experimenting with various additives.

Growing Tarragon horizontally as in from trays? -- Yes.

The MG works, but adds no carbon, the gelatin recipe has ended up the better version so far, it has better but slower growth. < Still tweaking.

Yes of coarse be aseptic for proper work, I am 'testing' various materials and open air assembly.


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25044885 - 03/07/18 09:48 AM (2 years, 4 months ago)

Thanks, T-gel is a cool concept :laugh:

I checked and malt contains lower / free sugars like glucose as well as oligosaccharides called maltodextrins. If you can substitute for the other various micro-nutrients well enough it may be worth checking the difference between malt extract and pure maltodextrin.

Am just wondering what the real differences are in macro-nutrient uptake between bacteria and our fungal cultures and how to maximize that. May be good to use as much selective nutrients like higher carbohydrates or materials composed mostly of lignins?

And I guess you can apply a spectrum of different T-gels depending on whether you want selectivity for your lignocolous fungus or instead a bit more rapid growth? For rapid growth you would allow for small amounts of easily digestible sugars etc even though bacteria could utilize them?


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Solipsis]
    #25046138 - 03/07/18 02:45 PM (2 years, 4 months ago)

Indeed it's pretty fun to work with. The tea is essentially selective to laccase capable organisms.
I am also looking at other 'resistant' materials, that don't germinate aerial invaders.

Currently I am trialing agar with gelatin as the only additive, no tea.

----

Update: Tarragon oyster detects and utilizes animal proteins for both carbon and nitrogen-other.
I should have added a black dye to the media, as my surface is white, visibility is low.

Hopefully the media won't turn to a liquid due to the agar base.

The protease enzymes still function at pH 8.0.

----

Standard assembly turns to a liquid, the agar falls and sets at the bottom.
The gelatin sits on top and hardens much later, in a fridge.

Using gelatin with T-Gel works as normal.


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Edited by Ferather (03/09/18 01:16 PM)


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25052145 - 03/09/18 02:34 PM (2 years, 4 months ago)

The best recipe's are recipe 6 then 2, after 4-5 days.

----

Recipe 1: Added 2g sucrose and 0.1g MG, per 100g extract.  < Got mold after 4-5 days, healthy.
Recipe 2: Added 2g 240B gelatin, per 100g extract.  < Slow for 4-5 days, medium speed, very populated, healthy.

Recipe 3: Added 1.6g of sucrose, per 100g extract.  < Not much change, medium weak growth, healthy.
Recipe 4: Added 0.1g MG, per 100g extract.  < Slightly slower, very populated, healthy.

Recipe 5: Added 0.2g MG, per 100g extract.  < Negative impact to health and speed, needs more carbon.
Recipe 6: Added 2g gelatin and 0.2g MG, per 100g extract.  < Very slow, slightly odd for 4-5 days then very healthy.

----

No contamination germinated with added gelatin, MG, or gelatin and MG.
Too much MG without an added carbon source causes a problem.


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Edited by Ferather (03/09/18 04:42 PM)


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25052177 - 03/09/18 02:48 PM (2 years, 4 months ago)

Suggested recipe for gelatin and T-Gel:

100g > Water  |  2.4g > Agar Powder  |  1.6g > Black Tea |  0.8g > 240B Gelatin (See Here).


I used the microwave oxidization assembly method, it sets as normal.

Add the gelatin after the cook, stir and reheat for 20 seconds.
The media will change from lightly cloudy to normal.

Tip: Reheat the media when it's hand cool.

----

Next test.

Recipe 7: Added 0.8g gelatin and 0.08g MG, per 100g extract.


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Edited by Ferather (03/09/18 04:59 PM)


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25053713 - 03/10/18 05:30 AM (2 years, 4 months ago)

Just about managed to get some images of Recipe 6, sorry for the quality.

       


Here is Recipe 2, not as good as Recipe 6.

   


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Edited by Ferather (03/11/18 07:13 AM)


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25053820 - 03/10/18 07:05 AM (2 years, 4 months ago)

Here is a side scraping from T-Gel Recipe 2 to Recipe 7.
Recovery after 4 hours, full potential after 10.



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Edited by Ferather (03/11/18 07:48 AM)


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25064859 - 03/15/18 07:57 AM (2 years, 4 months ago)

I'm ordering a new culture of Tarragon from the only vendor I can find (same one).
This time it will be taken directly to T-Gel, for cleaning (not clean).

I'm refraining from sending new transfers until then.


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25066381 - 03/15/18 05:33 PM (2 years, 4 months ago)

The new dowels should turn up this weekend. This time I did not order the rye spawn, hoping the dowels are better.
Previously I used the spawn on a substrate expecting normal results, then taking a transfer after.

At the time I was using seaweed as an additive, which contains additional bacteria.

----

T-Gel succesfully cleaned the visible yeast-bacteria combo, but not the added seaweed bacteria.
I believe the added bacteria is Archaea, which can produce laccase and grow on T-Gel.

The Archaea-other, produces dark patching on T-Gel and WL-Tek, hard to see.

I'm hoping the vendor dowels will be free from laccase invaders.

----

I will replace previous wedges on completion (free).



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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather] * 1
    #25068983 - 03/16/18 03:00 PM (2 years, 4 months ago)

Thank you Ferather,
I am impressed. Tarragon dowels seemed to be axenic. purchased 2 years ago.
Some observations: Tarragon oyster grew huge kombucha like blobs in liquid culture stored at 2 degrees Celsius. But substrates fruit after exposure to temperatures bellow 10(?) Celsius at any stage of incubation. Temperature is an approximation. Incubation is greatly accelerated at temperatures above 20 Celsius but I have never incubated it above 23 so...

I always wanted to isolate some new cultures from spores but always busy, now you gave me the inspiration...

Keep it coming!


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: ScotTony]
    #25069130 - 03/16/18 03:59 PM (2 years, 4 months ago)

No problem, I am still annoyed I cannot find a wild one, nor another vendor. Solarity also warned me about the current supplying vendor.

----

The kombucha you describe is similar to what I got the first time the transfer culture was placed onto T-Gel.

           

           

           


This is the same peg, onto another T-Gel recipe, I cant remember which one:

   


I cleaned the bacteria-yeast, but not the added Archaea-other:

   

Notice the patching around the wedge.

----
----

PM: Can I still use the wedges you sent me?

You can use the wedges I sent, it could cleanup entirely, fruiting and taking spores is not an issue.
Be prepared to get visible browning, in the spawn and substrate, and some loss of yield.

Any containers will need proper sterilization after, or be disposed of.

There is not much Archaea-other on the wedges.

----

Source, organic liquid seaweed, can be used on vegetables:

   

Not ideal for primary "decomposers".

----

TravelAgency who received a pre T-Gel peg, said the fruits where unique and special.
He managed to produce good yield even without the T-Gel cleaning I used.

I believe he and another made light attempts at cleaning it.


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Edited by Ferather (03/16/18 04:40 PM)


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25073192 - 03/18/18 01:19 PM (2 years, 4 months ago)

those look nce.

still have any left?


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Strangebolete]
    #25075016 - 03/19/18 08:50 AM (2 years, 4 months ago)

I do, but I am still waiting on a fresh sample, which apparently is tomorrow.
I also have the same Golden oyster as the images below.


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25080698 - 03/21/18 06:46 PM (2 years, 4 months ago)

Hey I really appreciate you sending me a culture! I wanted to ask in your opinion the best agar recipe to add the wedge to?


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Xerbia]
    #25080776 - 03/21/18 07:16 PM (2 years, 4 months ago)

I appreciate the culture as well. Though it seems customs
opened it and took the contents!


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: myceliups]
    #25084314 - 03/23/18 08:04 AM (2 years, 4 months ago)

Quote:

Xerbia said:
Hey I really appreciate you sending me a culture! I wanted to ask in your opinion the best agar recipe to add the wedge to?




T-Gel, T-Gel + Malt extract, Malt extract. You should see regeneration after 4-8 hours, and radial recovery after 12-18 hours.

----

Quote:

myceliups said:
I appreciate the culture as well. Though it seems customs
opened it and took the contents!




I will send you new ones once my new sample's have arrived, they have not yet, and when I have made a clean plate.

----

Export from another forum:

I'm going to throw off some of your very well researched and documented findings-theories. If you are talking about lignicolous mycelium you can also add polyphenols.
If we take for example lignin as the phenol, it contains no glucose, no sugars whatsoever, however lignicolous mycelium will receive carbon and other.

T-Gel contains about 0.01g of natural sugar per 200g of media, this is insignificant, the phenols are the main source of carbon, not sugars.
The mycelium will cleave the phenols, produce solubles, absorb them, and then continue "at will" to produce [X] material(s).


T-Gel + MG (phenols + macro-micro nutrients) had a significant impact to growth.
T-Gel + sucrose (phenols + sugars) had no significant impact to growth.

This can only be explained by utilization of phenols as carbon.

----

T-Gel:

Tip: Lignin is also a phenol based material

Tea leaves, Ready-to-drink.

Proteins, varies:  [ Cx | Hx | Ox | Nx ] x. -- Trace, minimal.
Polyphenols, varies:  [ C6 | H5 | O ] x. -- Phenols.
Cellulose: [ C6 | H10 | O5 ] x. -- Trace, debris.

Theobromine:  C7 | H8 | N4 | O2. -- Phenol.
Theophylline:  C7 | H8 | N4 | O2. -- Phenol.
Caffeine:  C8 | H10 | N4 | O2. -- Phenol.
Theaflavin:  C29 | H24 | O12. -- Phenol.
Tannins:  C76 | H52 | O46. -- Phenol.
Catechin:  C15 | H14 | O6. -- Phenol.

Carotene:  C40 | H56. -- Hydrocarbon.


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25084380 - 03/23/18 08:41 AM (2 years, 4 months ago)

Materials - general:


Glycogen: [C6]--[H10]--[O5], (contains glucose).
Ergosterol: [C28]--[H44]--[O], (essential).


Cellulose: [C6]--[H10]--[O5] x z, (same as glycogen, contains glucose).
Starch: [C6]--[H10]--[O5] x z, (same as glycogen, contains glucose).
Phenols: [C6]--[H5]--[O] x z, (phenols vary, not glucose based).
Sugar: [Cz]--[H2]z--[Oz], (sugar varies, ideally use glucose).

Protein: [Cz]--[Hz]--[Nz]--[Oz] x z, (contains both carbon and nitrogen).


z = Number of total units linked.

----

Enzymes

Disclaimer: Optimal pH varies with the organism, and it's enzymes.

Cellulase (cellulose): 4.5-8.5.
Laccase (phenols): 5.5-6.0.
Amylase (starch): 3.0-7.0.

Protease (protein): 2.5-11.


For cellulase there are four types:

Alkaline cellulase: 7.2-8.5.
Neutral cellulase: 6.0-8.0.
Hybrid cellulase: 4.5-7.0.
Acid cellulase: 4.5-5.0.


For amylase there are three types:

α-Amylase: 6.7-7.0.
β-Amylase: 4.0-5.0.
γ-Amylase: 3.0.


Laccase remains stable at pH 5.0-10.0.


For protease there are three types:

Alkaline protease: 7.0-11.0.
Neutral protease: 6.0-9.0.
Acid protease: 2.5-4.0.

----



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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25110631 - 04/03/18 10:26 AM (2 years, 4 months ago)

The vendor has apologized for the delay, most likely making a fresh batch. While I wait for that I decided to try 10% bleach peg's as transfer tools.
10% bleach will kill-inhibit bacteria-yeast-archaea, so it should kill-prevent the archaea that I currently have as an invader organism.

The multi-cellular mycelium can distribute materials to and from the peg, allowing it to tolerate the 10% bleach.
Unicellular organisms should die on contact, or be restricted, the pegs also provide an airy surface.

Here are some images of the pegs 2.5 days after placement, a little slow but working.

           

The mycelium appears to be thicker and more populated around the pegs.


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25111001 - 04/03/18 01:19 PM (2 years, 4 months ago)

           


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25111715 - 04/03/18 05:50 PM (2 years, 4 months ago)

Crazy how fast this culture colonizes. Just put a wedge off clean agar onto grains 2 days ago, and it's already 30-40% colonized. Insane!


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Xerbia]
    #25113769 - 04/04/18 01:39 PM (2 years, 3 months ago)

What grain did you use, and how much? How many wedges did you use?

Here are today's bleach peg images, slow as expected:

   


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25114020 - 04/04/18 03:58 PM (2 years, 3 months ago)

Quote:

Ferather said:
What grain did you use, and how much? How many wedges did you use?

Here are today's bleach peg images, slow as expected:

   




Well I put the wedge you sent on a plate of agar which grew out fast. Then cut a tiny wedge out of that and put in 1qt of oats. It’s about 60% colonized now without any shakes.


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Xerbia]
    #25114113 - 04/04/18 04:44 PM (2 years, 3 months ago)

Outstanding, what was the agar recipe?

I will be transferring these pegs to T-Gel soon, after that it can be any recipe.

:thumbup:


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather] * 1
    #25114203 - 04/04/18 05:31 PM (2 years, 3 months ago)

Quote:

Ferather said:
Outstanding, what was the agar recipe?

I will be transferring these pegs to T-Gel soon, after that it can be any recipe.

:thumbup:




Just potato flakes, agar, and karo


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Xerbia]
    #25115917 - 04/05/18 01:18 PM (2 years, 3 months ago)

Ok, so glucose and glucose-fructose. The interesting thing is the King oyster I sent to mattisfat also ended up more prolific than normal.
I'm guessing the conversion of the phenols in T-Gel to essential carbon, has improved the internal BE of the mycelium.

Technically, the phenols would be converted into glucose-other, so utilization of glucose may have improved.

----

Here are today's images of Tarragon oyster on T-Gel and the 10% bleach pegs.
Tarragon officially tolerates and grows on the pegs, I can transfer.

   

Cellulose is a glucan.


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather] * 1
    #25116047 - 04/05/18 02:11 PM (2 years, 3 months ago)

WOOT!


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather] * 1
    #25116062 - 04/05/18 02:18 PM (2 years, 3 months ago)

The real question IMO is whether the opposite works just as well. Fungi like switching substrate to some extent (though very big changes can apparently stump them temporarily I guess), could just be that variety is 'the spice of life' in that a broad spectrum of nutrition is offered.

It makes sense that including polymers of sugars and short chains or monomers in the medium is best similar to how it is easy for our bodies: have some readily available and use the rest for the slow burn.

Both glucose and fructose seem like the best sugars for fungi to me considering both are pretty easily used in chitin synthesis.

I guess you may be right Ferather, about aromatic degradation pathways still fueling the krebs cycle. And I guess this is also why at least supplementing one of these energy sources with the other should work best. In wood the potential for both is present.

I guess this suggests that you wanna incorporate a little bit of phenols in a medium otherwise based on sugars.

And yeah pretty crazy culture, it climbs the walls :laugh:


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Solipsis]
    #25116156 - 04/05/18 03:12 PM (2 years, 3 months ago)

Indeed, wood naturally will be cellulose-phenol based (in general, there are more materials), about 50% carbon and 0.1% nitrogen (weak).

From reading there is also energy storage based phenols, which can contain sugar as the backbone and-or bond.
Phenols come in a large variety, simple and complex, some are acids, some contain no sugar.

Note: Proteins contain glucans-other and fixated nitrogen.


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25116235 - 04/05/18 03:49 PM (2 years, 3 months ago)

I was just checking the links being used in my journal are still active, when I noticed the link in my WL-Tek guide has been updated recently.

Updated link: The Effects of Temperature and Nutritional Conditions on Mycelium Growth of Two Oyster Mushrooms.

It seems the author has added additional images for more data and better reading, worth a look.
The carbon and nitrogen test results are particularly interesting to look at.


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25117405 - 04/06/18 01:54 AM (2 years, 3 months ago)

I shook this early this morning before work, and now as I’m heading to bed I check it and it’s fully recovered and nearly 100% colonized. This is the most aggressive culture I’ve ever used!



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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Xerbia]
    #25118743 - 04/06/18 03:40 PM (2 years, 3 months ago)

Yes it has a very fast recovery time, it takes about 4-8 hours to recover, usually.

I will be transferring a peg tomorrow, today's images:

   


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather] * 1
    #25125160 - 04/09/18 08:44 AM (2 years, 3 months ago)

The bleach pegs appeared to have worked as intended first time round, however once colonized I will repeat one more time.
Currently I am using T-Gel plain, no gelatin or other additives, population and rate of growth have improved.

Using a visual estimate, the mycelium has improved by 25%, growth is thick, populated and fast.
The multi-cellular mycelium tolerated the 10% bleach, there are no visible invaders.

----

Household bleach:

Disinfectant: Sodium Hypochlorite 4.47g per 100g.
Anionic Surfactant: Less than 5%.


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather] * 1
    #25126297 - 04/09/18 04:57 PM (2 years, 3 months ago)

Looks like my next giveaway will be bleach pegs, in a centrifuge vial.

   


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25126311 - 04/09/18 05:03 PM (2 years, 3 months ago)

WOOT! Are they ready yet?

Are they ready yet?

Are they ready now?

How about now?
Fine, I'll wait.


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Bry Bry]
    #25126341 - 04/09/18 05:17 PM (2 years, 3 months ago)

I will let you all know when, I'm planning to make 10 pegs, 2 pegs a person.

:thumbup:


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25126351 - 04/09/18 05:20 PM (2 years, 3 months ago)

:thumbup:


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25126436 - 04/09/18 05:48 PM (2 years, 3 months ago)

Quote:

Ferather said:
Looks like my next giveaway will be bleach pegs, in a centrifuge vial.

   




Is the agar in those tubes just water and agar to keep the peg from drying out or is it some kind of media that the mycelium will grow on too?

I've been wanting to try this peg method but haven't even cut the toothpics yet.  Looks like it could be a great way to ship cultures.  If you could ship them in a small plastic bag instead of the centrifuge vial, it could make international shipping cheaper but they might dry out too fast.


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Mycosperiment]
    #25126449 - 04/09/18 05:52 PM (2 years, 3 months ago)

If the peg is well hydrated and in a sealed container, it shouldn't dry out unless you are sending somewhere with 30 day or longer snail mail delivery. Then you can put a little agar in the bottom to keep it lively.


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Mycosperiment]
    #25126460 - 04/09/18 05:56 PM (2 years, 3 months ago)

There is no agar in those tubes, just wood pegs with mycelium on it, the condensate is because they where kept in a fridge.
The shipping will cost the same for vials or bags, in fact, I put the vials into a zip-lock bags, extra safety.

The pegs are cut with wire cutters, you can also snap them, PC them in a small container.
You can add agar to the bottom of the vials, using folded card with holes.

The added agar will prevent rolling, touching and drying out.


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25127242 - 04/09/18 11:17 PM (2 years, 3 months ago)

I would be very interested in trying a culture.. :smile:


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25127632 - 04/10/18 06:42 AM (2 years, 3 months ago)

Quote:

Ferather said:
There is no agar in those tubes, just wood pegs with mycelium on it, the condensate is because they where kept in a fridge.
The shipping will cost the same for vials or bags, in fact, I put the vials into a zip-lock bags, extra safety.

The pegs are cut with wire cutters, you can also snap them, PC them in a small container.
You can add agar to the bottom of the vials, using folded card with holes.

The added agar will prevent rolling, touching and drying out.




If shipping is the same for vials or bags then vials would obviously be the best option.  I was thinking the little pegs could be put in a small bag (maybe an impulse sealed bag) and sent in a normal envelope.

I've never shipped anything out of the USA but assume the cost would be pretty high.  How much does it cost to ship your pegs from the UK to the USA?

I'm going to try sterilizing some pegs and see how some other oyster cultures will grow on them.


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Mycosperiment]
    #25127991 - 04/10/18 10:03 AM (2 years, 3 months ago)

£1.20 for two first class stamps.


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OfflineFerather
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25128444 - 04/10/18 01:11 PM (2 years, 3 months ago)

10% bleach peg to plain T-Gel, no gelatin:

Working as intended, the mycelium visibly looks healthier. The mycelium has pushed about 15mm from the peg.
The growth is radial, so the full diameter is about 30mm, the growth has changed around the edge.

The new growth is more populated, thicker and more visible than the starter growth.

----

Side note:

The added gelatin improves BE by 50% in comparison to plain T-Gel.


--------------------
                   
--------------------
https://www.shroomery.org/forums/postlist.php/Journal/350881 - Ferather's Journal, guides, recipes and more [2019]
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RGM: Rift Game Manager - DPS meter for Rift, with absorb to heal converter and data export [2017]
Commander: [Cygwin + Cmder] - Customize Android, Setup VirtualBox, plus more [2019]


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OfflineFerather
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25130906 - 04/11/18 01:10 PM (2 years, 3 months ago)

10% bleach peg to plain T-Gel, no gelatin:

Total growth is currently 45mm in diameter, +15mm, +7.5mm center to edge, since yesterday, and still accelerating.
PSA + ammonium chloride produced a 50mm diameter after 4 days for Pleurotus ostreatus (see here).

The T-Gel plain, without additives, is relatively close in speed, but can take more nitrogen.
Adding MG nutrients or adding proteins, had an almost identical effect on BE.


--------------------
                   
--------------------
https://www.shroomery.org/forums/postlist.php/Journal/350881 - Ferather's Journal, guides, recipes and more [2019]
--------------------
RGM: Rift Game Manager - DPS meter for Rift, with absorb to heal converter and data export [2017]
Commander: [Cygwin + Cmder] - Customize Android, Setup VirtualBox, plus more [2019]


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OfflineFerather
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25130965 - 04/11/18 01:42 PM (2 years, 3 months ago)

10% bleach peg to plain T-Gel, no gelatin:

Here are some images, sorry for the quality, condensate due to temperature changes in the room.
Growth is almost perfectly radial, and uniform, the outer growth is improving.

       

----

Now some visual side by sides, various days, not timed.

----

T-Gel with added gelatin and added MG nutrients:

   

----

T-Gel with added gelatin:

   


--------------------
                   
--------------------
https://www.shroomery.org/forums/postlist.php/Journal/350881 - Ferather's Journal, guides, recipes and more [2019]
--------------------
RGM: Rift Game Manager - DPS meter for Rift, with absorb to heal converter and data export [2017]
Commander: [Cygwin + Cmder] - Customize Android, Setup VirtualBox, plus more [2019]


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OfflineFerather
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25144923 - 04/17/18 06:44 AM (2 years, 3 months ago)

I told the vendor of the Tarragon dowels I am patient and would rather a good batch.
Today they have arrived, I will be using this culture for backup samples.

   

Comes with a fact-help sheet.


--------------------
                   
--------------------
https://www.shroomery.org/forums/postlist.php/Journal/350881 - Ferather's Journal, guides, recipes and more [2019]
--------------------
RGM: Rift Game Manager - DPS meter for Rift, with absorb to heal converter and data export [2017]
Commander: [Cygwin + Cmder] - Customize Android, Setup VirtualBox, plus more [2019]


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OfflineFerather
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Posts: 5,837
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25144924 - 04/17/18 06:44 AM (2 years, 3 months ago)

10% bleach peg, WL-Tek + 10% CaCO3, trial:

Started another trial, in a similar way to the 10% bleach peg. I added 2.5g of ultra fine calcium carbonate, for 25g of paper pellets, to my water.
The mixture of CaCO3, Miracle-gro and yeast nutrients produces no side effect gasses, or anomaly's, it mixes perfectly.

If I added bleach to MG and YN, toxic gasses would be released, do not do it, literally ever!

The end pH is an average of 8.5, the unreacted CaCO3 will convert overtime.
Water retention has increased slightly, the smell is cleaner.

   


--------------------
                   
--------------------
https://www.shroomery.org/forums/postlist.php/Journal/350881 - Ferather's Journal, guides, recipes and more [2019]
--------------------
RGM: Rift Game Manager - DPS meter for Rift, with absorb to heal converter and data export [2017]
Commander: [Cygwin + Cmder] - Customize Android, Setup VirtualBox, plus more [2019]


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