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OfflineFerather
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Xerbia]
    #25113769 - 04/04/18 01:39 PM (2 years, 3 months ago)

What grain did you use, and how much? How many wedges did you use?

Here are today's bleach peg images, slow as expected:

   


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OfflineXerbia
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25114020 - 04/04/18 03:58 PM (2 years, 3 months ago)

Quote:

Ferather said:
What grain did you use, and how much? How many wedges did you use?

Here are today's bleach peg images, slow as expected:

   




Well I put the wedge you sent on a plate of agar which grew out fast. Then cut a tiny wedge out of that and put in 1qt of oats. It’s about 60% colonized now without any shakes.


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OfflineFerather
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Xerbia]
    #25114113 - 04/04/18 04:44 PM (2 years, 3 months ago)

Outstanding, what was the agar recipe?

I will be transferring these pegs to T-Gel soon, after that it can be any recipe.

:thumbup:


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OfflineXerbia
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather] * 1
    #25114203 - 04/04/18 05:31 PM (2 years, 3 months ago)

Quote:

Ferather said:
Outstanding, what was the agar recipe?

I will be transferring these pegs to T-Gel soon, after that it can be any recipe.

:thumbup:




Just potato flakes, agar, and karo


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OfflineFerather
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Xerbia]
    #25115917 - 04/05/18 01:18 PM (2 years, 3 months ago)

Ok, so glucose and glucose-fructose. The interesting thing is the King oyster I sent to mattisfat also ended up more prolific than normal.
I'm guessing the conversion of the phenols in T-Gel to essential carbon, has improved the internal BE of the mycelium.

Technically, the phenols would be converted into glucose-other, so utilization of glucose may have improved.

----

Here are today's images of Tarragon oyster on T-Gel and the 10% bleach pegs.
Tarragon officially tolerates and grows on the pegs, I can transfer.

   

Cellulose is a glucan.


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OfflineBry Bry
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather] * 1
    #25116047 - 04/05/18 02:11 PM (2 years, 3 months ago)

WOOT!


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OnlineSolipsis
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather] * 1
    #25116062 - 04/05/18 02:18 PM (2 years, 3 months ago)

The real question IMO is whether the opposite works just as well. Fungi like switching substrate to some extent (though very big changes can apparently stump them temporarily I guess), could just be that variety is 'the spice of life' in that a broad spectrum of nutrition is offered.

It makes sense that including polymers of sugars and short chains or monomers in the medium is best similar to how it is easy for our bodies: have some readily available and use the rest for the slow burn.

Both glucose and fructose seem like the best sugars for fungi to me considering both are pretty easily used in chitin synthesis.

I guess you may be right Ferather, about aromatic degradation pathways still fueling the krebs cycle. And I guess this is also why at least supplementing one of these energy sources with the other should work best. In wood the potential for both is present.

I guess this suggests that you wanna incorporate a little bit of phenols in a medium otherwise based on sugars.

And yeah pretty crazy culture, it climbs the walls :laugh:


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OfflineFerather
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Solipsis]
    #25116156 - 04/05/18 03:12 PM (2 years, 3 months ago)

Indeed, wood naturally will be cellulose-phenol based (in general, there are more materials), about 50% carbon and 0.1% nitrogen (weak).

From reading there is also energy storage based phenols, which can contain sugar as the backbone and-or bond.
Phenols come in a large variety, simple and complex, some are acids, some contain no sugar.

Note: Proteins contain glucans-other and fixated nitrogen.


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OfflineFerather
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25116235 - 04/05/18 03:49 PM (2 years, 3 months ago)

I was just checking the links being used in my journal are still active, when I noticed the link in my WL-Tek guide has been updated recently.

Updated link: The Effects of Temperature and Nutritional Conditions on Mycelium Growth of Two Oyster Mushrooms.

It seems the author has added additional images for more data and better reading, worth a look.
The carbon and nitrogen test results are particularly interesting to look at.


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OfflineXerbia
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25117405 - 04/06/18 01:54 AM (2 years, 3 months ago)

I shook this early this morning before work, and now as I’m heading to bed I check it and it’s fully recovered and nearly 100% colonized. This is the most aggressive culture I’ve ever used!



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OfflineFerather
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Xerbia]
    #25118743 - 04/06/18 03:40 PM (2 years, 3 months ago)

Yes it has a very fast recovery time, it takes about 4-8 hours to recover, usually.

I will be transferring a peg tomorrow, today's images:

   


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OfflineFerather
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather] * 1
    #25125160 - 04/09/18 08:44 AM (2 years, 3 months ago)

The bleach pegs appeared to have worked as intended first time round, however once colonized I will repeat one more time.
Currently I am using T-Gel plain, no gelatin or other additives, population and rate of growth have improved.

Using a visual estimate, the mycelium has improved by 25%, growth is thick, populated and fast.
The multi-cellular mycelium tolerated the 10% bleach, there are no visible invaders.

----

Household bleach:

Disinfectant: Sodium Hypochlorite 4.47g per 100g.
Anionic Surfactant: Less than 5%.


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OfflineFerather
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather] * 1
    #25126297 - 04/09/18 04:57 PM (2 years, 3 months ago)

Looks like my next giveaway will be bleach pegs, in a centrifuge vial.

   


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OfflineBry Bry
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25126311 - 04/09/18 05:03 PM (2 years, 3 months ago)

WOOT! Are they ready yet?

Are they ready yet?

Are they ready now?

How about now?
Fine, I'll wait.


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OfflineFerather
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Bry Bry]
    #25126341 - 04/09/18 05:17 PM (2 years, 3 months ago)

I will let you all know when, I'm planning to make 10 pegs, 2 pegs a person.

:thumbup:


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OfflineBry Bry
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25126351 - 04/09/18 05:20 PM (2 years, 3 months ago)

:thumbup:


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OfflineMycosperiment
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25126436 - 04/09/18 05:48 PM (2 years, 3 months ago)

Quote:

Ferather said:
Looks like my next giveaway will be bleach pegs, in a centrifuge vial.

   




Is the agar in those tubes just water and agar to keep the peg from drying out or is it some kind of media that the mycelium will grow on too?

I've been wanting to try this peg method but haven't even cut the toothpics yet.  Looks like it could be a great way to ship cultures.  If you could ship them in a small plastic bag instead of the centrifuge vial, it could make international shipping cheaper but they might dry out too fast.


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OfflineBry Bry
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Mycosperiment]
    #25126449 - 04/09/18 05:52 PM (2 years, 3 months ago)

If the peg is well hydrated and in a sealed container, it shouldn't dry out unless you are sending somewhere with 30 day or longer snail mail delivery. Then you can put a little agar in the bottom to keep it lively.


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OfflineFerather
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Mycosperiment]
    #25126460 - 04/09/18 05:56 PM (2 years, 3 months ago)

There is no agar in those tubes, just wood pegs with mycelium on it, the condensate is because they where kept in a fridge.
The shipping will cost the same for vials or bags, in fact, I put the vials into a zip-lock bags, extra safety.

The pegs are cut with wire cutters, you can also snap them, PC them in a small container.
You can add agar to the bottom of the vials, using folded card with holes.

The added agar will prevent rolling, touching and drying out.


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https://www.shroomery.org/forums/postlist.php/Journal/350881 - Ferather's Journal, guides, recipes and more [2019]
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Commander: [Cygwin + Cmder] - Customize Android, Setup VirtualBox, plus more [2019]


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OfflineFungalDane
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25127242 - 04/09/18 11:17 PM (2 years, 3 months ago)

I would be very interested in trying a culture.. :smile:


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