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OfflineFerather
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: FishLevelMidnight] * 1
    #25022927 - 02/26/18 09:45 AM (2 years, 5 months ago)

All wedges have been sent, I will do more wedges soon.


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Offlinechobumms
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25025455 - 02/27/18 04:05 AM (2 years, 5 months ago)

Quote:

Ferather said:
All wedges have been sent, I will do more wedges soon.




I'm looking forward to it!


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InvisibleHumble Newcomer
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: chobumms]
    #25025868 - 02/27/18 09:26 AM (2 years, 5 months ago)

Are you mailing to the states Ferather? If so I'd like to get on this. I could reply in kind and send you one of my new acquisitions that will be ready in the coming weeks, maybe when you have a few more ready.


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OfflineFishLevelMidnightS
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Humble Newcomer]
    #25025871 - 02/27/18 09:27 AM (2 years, 5 months ago)

If anyone is willing to spread the love and send a sample of the tarragon oyster after they grow it out let me know! I have some thing for trade.

Thanks for everything you do Fer


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OfflineFerather
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: FishLevelMidnight]
    #25025908 - 02/27/18 09:47 AM (2 years, 5 months ago)

I will do more wedges soon on the updated T-Gel, I am using the microwave assembly, and adding my additives after the cook.
Currently I have 3 plates made on different days, a visual comparison is being made, on population and health.

Microwaving the pre-extract causes it oxidize, in a similar fashion to laccase enzymes.
The conversion also causes to agar to set very hard, without any sugars.

----

Control: Plain, no additives.

Recipe 1: Added 2g sucrose and 0.1g MG, per 100g extract.
Recipe 2: Added 2g 240B gelatin, per 100g extract.

----

Previous tests, using the old assembly (soft, not fully hardened).


Plain, no additives:

   


With added macro-micro nutrients (MG):

   


With added 240B (bloom) gelatin:

   


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Offlinetscott1632
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: FishLevelMidnight]
    #25027038 - 02/27/18 06:35 PM (2 years, 5 months ago)

Pumped


Edited by tscott1632 (02/27/18 06:36 PM)


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OfflineFerather
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: tscott1632]
    #25028715 - 02/28/18 01:00 PM (2 years, 5 months ago)

T-Gel Recipe 1 has germinated and grown out a random mycelium after only 4.5 days.
Recipe 2 is yet to contaminate and is producing growth equal to Recipe 1.

Bottom: Control, Middle: Recipe 1, Top: Recipe 2.



----

It's lignicolous mold, there is a green-blue patch appearing. It's also penetrative (see below, right side - internal white).

----

Surface growth is more populated and aerial with Recipe 1 and Recipe 2, very similar results.
The both samples have penetrative growth, through the wedge and the main agar.

Recipe 1, is 2.5 days ahead of Recipe 2 in terms of sample assembly.

       

Sorry for the quality, not the best containers for images.


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Edited by Ferather (02/28/18 03:17 PM)


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OfflineFerather
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25029428 - 02/28/18 03:59 PM (2 years, 5 months ago)

Some data and notes for cultivators:

The T-Gel plate with added MG, but no sucrose, did not contaminate from open air, while with added sucrose did, rapidly.
Gelatin (change to 100g) is about 85-95% proteins, nutritional proteins count both as carbon and nitrogen.

Recipe 1, is producing equal results to Recipe 2 due to the addition of the sucrose as carbon.
In short the mixture of both carbon and nitrogen acts like adding proteins, in a way.

----

Tarragon oyster will also utilize other carbon sources with MG (WL-Tek).
It decay's the cellulose as carbon (no sugar, no starch).

   

Use clean spawn, wait 2-3 weeks.


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OfflineFerather
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25029497 - 02/28/18 04:30 PM (2 years, 5 months ago)

Also, I forgot to mention Tarragon oyster will also utilize lime (CaCO3) as a carbon source, when soluble.


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InvisibleHumble Newcomer
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25030279 - 02/28/18 10:15 PM (2 years, 5 months ago)

How long was it exposed to open air? That's cool


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OfflineFerather
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Humble Newcomer]
    #25030915 - 03/01/18 05:12 AM (2 years, 5 months ago)

These 3 plates where poured open to air, and set open to air, that's about 2 hours.

The lignicolous mold is very healthy, very populated and well fed.
Cubensis also germinates on sucrose + MG, in 4 days.



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InvisibleHumble Newcomer
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25031025 - 03/01/18 06:52 AM (2 years, 5 months ago)

So t gel made with MG and no added sucrose, was poured hot in a dish in open air, allowed to set in open air approximately 2 hours later, and to this date it still hasnt germinated any airborne spores/bacteria/yeast?

How long ago did you pour? The other plate with sucrose that contaminated quickly, it was poured at the same time?

:eek:


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OfflineFerather
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Humble Newcomer]
    #25032870 - 03/02/18 07:02 AM (2 years, 5 months ago)

Not quite that much of a test, I am testing open to air assembly, with the push of setting the agar open to air.
All samples are inoculated with an oyster sample after fully setting, general data is recorded.

I am testing effects on growth, and chance to contaminate from assembly-other.
So far Recipe 2 is slower than Recipe 1, Recipe 1 has only mold.

All 3 samples are clear of visible bacteria-yeast.


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OfflineFerather
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25033305 - 03/02/18 10:43 AM (2 years, 5 months ago)

Recipe 1: Added 2g sucrose and 0.1g MG, per 100g extract.
Recipe 2: Added 2g 240B gelatin, per 100g extract.

Recipe 3: Added 1.6g of sucrose, per 100g extract.
Recipe 4: Added 0.1g MG, per 100g extract.

----

Control:

Plain T-Gel, no additives. Normal speed and population rate for T-Gel, no contamination.


Current results:

Recipe 1: Produced better growth, mold after 4.5 days, no bacteria-yeast.
Recipe 2: Negative impact to speed, no contamination.


Previous results:

Recipe 3: A tiny bit better in general growth, no contamination.
Recipe 4: Improved population, no contamination.


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OfflineFerather
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25033341 - 03/02/18 10:57 AM (2 years, 5 months ago)

Some people have received their wedges already, but they are European or close.
I realize I have not labelled the sample wedges, and isolate name.

Pleurotus euosmus (Tarragon Oyster): TAR01

----

I've ditched the moldy Recipe 1, and will be trying Recipe 5:

Recipe 5: Added 0.2g MG, per 100g extract.


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Edited by Ferather (03/02/18 12:41 PM)


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Offlinechobumms
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25037013 - 03/03/18 09:31 PM (2 years, 5 months ago)

Very interesting. Your oyster pics are beautiful


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OfflineFerather
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: chobumms]
    #25037558 - 03/04/18 06:13 AM (2 years, 4 months ago)

Thanks, Golden oyster loves: Sunlight + water (rain) + high humidity + high O2.
Tarragon oyster needs far less light, but it produces thicker stems.

Tarragon oyster will also fruit very well horizontally.

----

Parent:

Pleurotus cornucopiae (Branched Oyster).

Variations:

Pleurotus citrinopileatus (Golden Oyster).
Pleurotus euosmus (Tarragon Oyster).


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OfflineFerather
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25037795 - 03/04/18 09:17 AM (2 years, 4 months ago)

Recipe 5: Added 0.2g MG, per 100g extract:

Improved laccase production (more browning), I believe this is due to two reasons, enzymes are proteins, they are composed of C-H-N-O.
Laccase also contains copper, so it's C-H-N-O-Cu, the MG adds nitrogen and copper to the T-Gel media, which contains C-H-O.

In general laccase will produce C-H-O from phenol's, and the N-Cu from the added MG, more enzymes = more C-H-O.

Recipe 2, 4 and 5 produced an increase to population rate, thickness and overall growth potential.
The slowest of the three is Recipe 2, nutritional proteins provide C-H-O and N-Other.

----

Recipe 1: Added 2g sucrose and 0.1g MG, per 100g extract.
Recipe 2: Added 2g 240B gelatin, per 100g extract.

Recipe 3: Added 1.6g of sucrose, per 100g extract.
Recipe 4: Added 0.1g MG, per 100g extract.


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https://www.shroomery.org/forums/postlist.php/Journal/350881 - Ferather's Journal, guides, recipes and more [2019]
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OfflineFerather
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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25037833 - 03/04/18 09:35 AM (2 years, 4 months ago)

All of my samples produced less carbon based waste when the level of N-Other increased.

----

Next Test:

Recipe 6: Added 2g gelatin and 0.2g MG, per 100g extract.


--------------------
                   
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https://www.shroomery.org/forums/postlist.php/Journal/350881 - Ferather's Journal, guides, recipes and more [2019]
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Commander: [Cygwin + Cmder] - Customize Android, Setup VirtualBox, plus more [2019]


Edited by Ferather (03/04/18 03:35 PM)


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Re: Tarragon Oyster T-Gel Wedges [Giveaway] [Re: Ferather]
    #25044507 - 03/07/18 04:11 AM (2 years, 4 months ago)

I don't have malt extract so tweaked the T-gel recipe/concept a little before (and have in the process also created agar on which nothing seems to grow, lol), got a good one now though:

Around 150 ml water, simmer in it about 5-6g oats and a dash of bran, extract a teabag of black organic tea in it and strain carefully into erlenmeyer with 4(+) g agar powder suspended in about 50 ml cold / RT water, to make 200 ml agar.

I was already dying my medium for contrast - now that I have a few more colors I can color code for different media... although T-gel does this spontaneously by being brown. :wink:

I suspect that maltodextrine may be a suitable replacement for the oats or malt extract but not sure (I think oligosaccharides are mostly the point of the malt), but if I were to purchase new ingredients might as well go for the malt extract. Using yeast nutrition can provide micro-nutrients - mostly minerals and some other inorganics etc - bran surely has a richer organic micro-nutrient mix like including protein.

I was having a lot of tomentose growth on my PDA, and while growth wasn't necessarily slow it tended to be wispy initially (later consolidated), I believe I was allowing too much potato starch to be dissolved / suspended.

On the tweaked T-gel i mentioned I now am getting much more beautiful full white growth (that is, with Ovoids, subs, and now also gyms and Ps Mex though I have no idea how lignicolous it is as nobody grows truffles on sawdust lol) and it should work for gourmet woodlovers as well.

Might have to check differences between MG, bran and yeast nutrient (nutrivit)..

Growing Tarragon horizontally as in from trays?

By the way: while testing open air conditions might be interesting in *some* ways it seems like a bad plan because contaminants can still hitch a ride secretly even if they are not encouraged to grow and become dominant. Would you not expect problems once you go to spawn and bulk? Or are you just experimenting to see more acute sensitivity to seriously contaminate?
Just saying: you might wanna point out that you don't recommend open air conditions at this stage even with actual T-gel use if it is not meant for that.


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