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Mushrooms, Mycology and Psychedelics > Gourmet and Medicinal Mushrooms
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Different agar medium
    #1817180 -

hi there

Until now, I always made my agar medium following the PDA recipe
but then, I purchased some light malt extract and tried the MEA
none of my cultures look the same
oysters were used to be cottony
now the mycelium is so thin, I can almost see it
same goes for other mushrooms
now, I'm very sure I didn't mess up the recipe (I have a scale)

What could the problem be ?
the malt extract isn't good ? perhaps not enough ?
or is pda simply better?

another thing I can't figure out, what is neo-peptone ?
I heard it could improve the colonization speed
where could I buy it ?

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Re: Different agar medium [Re: MushMushi]
    #1817620 -

Ya know. I have noticed that too. The colonies of typically heavy mycelial species (on my supplemented PDA) are wispy and even hard to see on the MEA that I have made. Further, I have found that spore germination is poorer and slower on MEA when compared to my supplemented PDA. I think it has something to do with nutrient levels. You might alleviate the problem by spiking the MEA with oatmeal, yeast extract, and peptone. I have not yet tried this myself though.


--------------------
Listen! Do you smell something?

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Re: Different agar medium [Re: MushMushi]
    #1817627 -

www.fungi.com


--------------------
Raadt

-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--

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Re: Different agar medium [Re: Raadt]
    #1817741 -

???

I know www.fungi.com sells sterile culture media but I'm not looking to buy mea or pda powders

It's the only place that sells peptone ?


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Re: Different agar medium [Re: MushMushi]
    #1817823 -

You didn't look very hard.

http://www2.mailordercentral.com/fungi/P...M=1&MITEM=1

Peptone
Enhances mycelial growth of most saprophytic fungi. 100 gram bottles.
M400 $21.95


I'm not sure who else sells it. This is where I got mine.


--------------------
Raadt

-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--

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Re: Different agar medium [Re: Raadt]
    #1817860 -

I knew they sell it
why would I ask then "it's the ONLY place that sells peptone ?"
perhaps you didn't read my post carefully

does it really work ? it is for the hobbyist or the commercial grower ?
it seems pretty expensive for 100 grams


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Re: Different agar medium [Re: r05c03]
    #1817861 -

I think I'll go back to pda
perhaps supplement it with oatmeal

:grin: 

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Re: Different agar medium [Re: MushMushi]
    #1817898 -

I'm sorry. I must have misunderstood.

I just checked. Sporeworks sells it too. Cheaper as well.

http://www.sporeworks.com/media.html

good luck!


--------------------
Raadt

-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--

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Mushrooms, Mycology and Psychedelics > Gourmet and Medicinal Mushrooms
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