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Amazon Shop for: Agar, Malt Extract, Potato Dextrose, Scales

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InvisibleMushMushi
Registered: 08/23/02
Posts: 480
Loc: Canada
Different agar medium
    #1817180 - 08/15/03 03:58 PM (13 years, 8 months ago)

hi there

Until now, I always made my agar medium following the PDA recipe
but then, I purchased some light malt extract and tried the MEA
none of my cultures look the same
oysters were used to be cottony
now the mycelium is so thin, I can almost see it
same goes for other mushrooms
now, I'm very sure I didn't mess up the recipe (I have a scale)

What could the problem be ?
the malt extract isn't good ? perhaps not enough ?
or is pda simply better?

another thing I can't figure out, what is neo-peptone ?
I heard it could improve the colonization speed
where could I buy it ?


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Offliner05c03
The Slug Scourge
Registered: 01/06/02
Posts: 383
Loc: Indiana, US
Last seen: 12 years, 17 days
Re: Different agar medium [Re: MushMushi]
    #1817620 - 08/15/03 06:09 PM (13 years, 8 months ago)

Ya know. I have noticed that too. The colonies of typically heavy mycelial species (on my supplemented PDA) are wispy and even hard to see on the MEA that I have made. Further, I have found that spore germination is poorer and slower on MEA when compared to my supplemented PDA. I think it has something to do with nutrient levels. You might alleviate the problem by spiking the MEA with oatmeal, yeast extract, and peptone. I have not yet tried this myself though.


--------------------
Listen! Do you smell something?


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OfflineRaadt
nicht

Registered: 06/07/02
Posts: 2,104
Loc: azurescending
Last seen: 9 years, 3 months
Re: Different agar medium [Re: MushMushi]
    #1817627 - 08/15/03 06:11 PM (13 years, 8 months ago)



--------------------
Raadt

-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--


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InvisibleMushMushi
Registered: 08/23/02
Posts: 480
Loc: Canada
Re: Different agar medium [Re: Raadt]
    #1817741 - 08/15/03 06:43 PM (13 years, 8 months ago)

???

I know www.fungi.com sells sterile culture media but I'm not looking to buy mea or pda powders

It's the only place that sells peptone ?



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OfflineRaadt
nicht

Registered: 06/07/02
Posts: 2,104
Loc: azurescending
Last seen: 9 years, 3 months
Re: Different agar medium [Re: MushMushi]
    #1817823 - 08/15/03 07:19 PM (13 years, 8 months ago)

You didn't look very hard.

http://www2.mailordercentral.com/fungi/P...M=1&MITEM=1

Peptone
Enhances mycelial growth of most saprophytic fungi. 100 gram bottles.
M400 $21.95


I'm not sure who else sells it. This is where I got mine.


--------------------
Raadt

-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--


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InvisibleMushMushi
Registered: 08/23/02
Posts: 480
Loc: Canada
Re: Different agar medium [Re: Raadt]
    #1817860 - 08/15/03 07:42 PM (13 years, 8 months ago)

I knew they sell it
why would I ask then "it's the ONLY place that sells peptone ?"
perhaps you didn't read my post carefully

does it really work ? it is for the hobbyist or the commercial grower ?
it seems pretty expensive for 100 grams



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InvisibleMushMushi
Registered: 08/23/02
Posts: 480
Loc: Canada
Re: Different agar medium [Re: r05c03]
    #1817861 - 08/15/03 07:43 PM (13 years, 8 months ago)

I think I'll go back to pda
perhaps supplement it with oatmeal

:grin: 


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OfflineRaadt
nicht

Registered: 06/07/02
Posts: 2,104
Loc: azurescending
Last seen: 9 years, 3 months
Re: Different agar medium [Re: MushMushi]
    #1817898 - 08/15/03 08:01 PM (13 years, 8 months ago)

I'm sorry. I must have misunderstood.

I just checked. Sporeworks sells it too. Cheaper as well.

http://www.sporeworks.com/media.html

good luck!


--------------------
Raadt

-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--


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Amazon Shop for: Agar, Malt Extract, Potato Dextrose, Scales

Mushrooms, Mycology and Psychedelics >> Gourmet and Medicinal Mushrooms

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