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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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Pc petri dishes?
    #17852385 -

Hey all, I would like to start working with agar, so i went out and got a pressure cooker todat after reading that steam sterilizing isn't recommended for agar. I have polystyrene petri dishes. Can I just pour my agar in them and pc them? Or do i need to pc my agar before I pour it? Thanks for your help i have a lot to learn.


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If there's anything worth doing, it's worth doing right...  -  Hunter S. Thompson

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Re: Pc petri dishes? [Re: jwthefunguy]
    #17852406 -

PC your agar and then pour the dishes in a SAB or in front of a Flow Hood.

http://www.mushroomvideos.com/Agar-Petri-Dishes

If you PC your plastic dishes they will melt.


--------------------
                 
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***

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Re: Pc petri dishes? [Re: PussyFart]
    #17852424 -

Thanks, i was thinking they would but i figured i would ask of it was possible.


--------------------
If there's anything worth doing, it's worth doing right...  -  Hunter S. Thompson

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Re: Pc petri dishes? [Re: jwthefunguy]
    #17852447 -

I fill glass plates with unsterilised agar and PC them all the time.
Plastic plates melt. :awesomenod:


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¯\_(ツ)_/¯

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Re: Pc petri dishes? [Re: Tmethyl]
    #17854844 -

can u sterlize plastic plates in boiling water?


--------------------
Reishi (fruit) Double Extraction
http://www.shroomery.org/forums/showflat.php/Number/17728431

drying Reishi mycelium
http://www.shroomery.org/forums/showflat.php/Number/17727919

how to take Reishi Mycelium
http://www.shroomery.org/forums/showflat.php/Number/17727387

"THE PSYCHEDELIC EXPERIENCE IS THE TRUE RELIGION"

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Re: Pc petri dishes? [Re: King Reishi]
    #17854914 -

No.


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Re: Pc petri dishes? [Re: King Reishi]
    #17855117 -

King Reishi said:
can u sterlize plastic plates in boiling water?


....technically, different agar formulations require different amounts of pc times, depending on the nutrient base  SOme as few as 20 minutes, but i just do them all for 45 minutes (16 psi as i am at 3000 ft) to be safe.  make  sure you cool them down before pour;  i bring mine down to 55 degrees celcius.

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Re: Pc petri dishes? [Re: Amanita virosa]
    #17855343 -

i mean just the dishes. not with the agar in them.


--------------------
Reishi (fruit) Double Extraction
http://www.shroomery.org/forums/showflat.php/Number/17728431

drying Reishi mycelium
http://www.shroomery.org/forums/showflat.php/Number/17727919

how to take Reishi Mycelium
http://www.shroomery.org/forums/showflat.php/Number/17727387

"THE PSYCHEDELIC EXPERIENCE IS THE TRUE RELIGION"

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Re: Pc petri dishes? [Re: King Reishi]
    #17857755 -

I would be afraid the plastic dishes would warp in the boiling water, dunno, never boiled plastic petris before :smirk:

I think they're UV sterilized before you purchase them; if you're thinking about re-using plastic ones, I would be apprehensive about contamination (even if I had some UV sterilizing abilities at home) personally, I just bite the bullet and order new ones :rolleyes:


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- endo cabendo

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Re: Pc petri dishes? [Re: MonkeyJesusFresco]
    #17861888 -

sterile polystyrene petridishes are usually sterilized by gamma rays after packaging. they are generally intended for single use only.
once unsterile, there is no way for you to get polystyrene sterile again, so work wisely.

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Re: Pc petri dishes? [Re: Fruitbuddy]
    #17862044 -

Gamma rays are great, because they go through everything. For about $3 million, you can have your own gamma ray sterilizer. Not counting the cost of the cobalt-60. :smile:


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Liberté, égalité, humidité.

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Re: Pc petri dishes? [Re: Terry M]
    #17862138 -

Terry M said:
Gamma rays are great, because they go through everything. For about $3 million, you can have your own gamma ray sterilizer. Not counting the cost of the cobalt-60. :smile:


isn't that what happened to the Incredible Hulk?  It was a govt experiment.  They put Bill Bixby in a lead box and bombarded him with gamma rays.  Then everytime he go mad he turned into Lou Ferrigno

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Re: Pc petri dishes? [Re: Terry M]
    #17862238 -

Terry M said:
Not counting the cost of the cobalt-60. :smile:


somebody write a tek on cobalt-60 synthesis, please! :derp:

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Re: Pc petri dishes? [Re: Fruitbuddy]
    #17863870 -

If I ever save up  3million thats what I'll buy.


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Patience will help you keep your sanity.It will insure your success if you are patient in all aspects of mushroom growing.When you rush you are prone to make mistakes and all of your efforts are wasted.

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Re: Pc petri dishes? [Re: Amanita virosa]
    #17913160 -

Amanita virosa said:
Terry M said:
Gamma rays are great, because they go through everything. For about $3 million, you can have your own gamma ray sterilizer. Not counting the cost of the cobalt-60. :smile:


isn't that what happened to the Incredible Hulk?  It was a govt experiment.  They put Bill Bixby in a lead box and bombarded him with gamma rays.  Then everytime he go mad he turned into Lou Ferrigno


it was a cover up. he was really trying to re-use his agar dishes. the initial frustration of having to buy more made him turn into a big half deaf guy. sad day for science...



:wink:


--------------------
"Eternity is an awful long time to wait, especially near the end" - Woody Allen
"it's like that drug trip in that movie I saw when I was on that drug trip!" - Phillip J. Fry

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Re: Pc petri dishes? [Re: Fruitbuddy]
    #17917066 -

How about soaking them in bleach or H2O2 solution? I have a bunch of polystyrene petri dishes...

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Re: Pc petri dishes? [Re: cli_hlt]
    #17917109 -

cli_hlt said:
How about soaking them in bleach or H2O2 solution? I have a bunch of polystyrene petri dishes...


Your mileage may vary...but I have had very good success with soaking pre-used petris in bleach water until right before pouring the agar. I also did some experiments with 2oz and 3/4oz deli cups...no contams. Obviously I got lucky with that.

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Re: Pc petri dishes? [Re: Subverted]
    #17917115 -

Bleach does not kill all mold spores, neither does H2o2, neither does alcohol.

But heat does.


--------------------
                 
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***

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Re: Pc petri dishes? [Re: PussyFart]
    #17917264 -

Man, I'll never touch plastic plates again. Grrr. Glass it is. Just sent an order to my favorite supplyer. I can toast them until every contamintant turns into charcoal.

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Re: Pc petri dishes? [Re: PussyFart]
    #17920873 -

.
Notahacker420 said:
Bleach does not kill all mold spores, neither does H2o2, neither does alcohol.

But heat does.




What about lysol they claim to kill 99.9 percent of viruses and bacteria.I also just searched lysol and they claim to kill 99.9 of molds and fungi. Just a thought but maybe hold the can upside down spray all of the air out then puncture it pour the contents into a bowl to soak your petri's in. I still would suggest investing in Pyrex if you want to use them multiple times. They can be found for around $5 a piece.


--------------------
Patience will help you keep your sanity.It will insure your success if you are patient in all aspects of mushroom growing.When you rush you are prone to make mistakes and all of your efforts are wasted.

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 |  [ show all ]

Shop: Unfolding Nature Unfolding Nature: Being in the Implicate Order   Mushroom-Hut Substrate Bags   Sporeworks.EU Spores for European Microscopy   Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Cultivation Supplies

Mushrooms, Mycology and Psychedelics > Advanced Mycology
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