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 Pinhead
Oregano
Registered: 05/14/00
Posts: 1,819
Loc: Hootersville
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 Re: Agar/Petri dish prep. 
#305828 - 04/30/01 05:45 PM (21 years, 2 months ago) |
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It does gel in the jars,but remelts when I PC it in the petri dish.
I try to use up the contents of the jar,once opened..if not I put the top on loose and microwave.
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 Triple_3
member
Registered: 03/21/01
Posts: 99
Last seen: 21 years, 7 days
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Re: Agar/Petri dish prep. [Re: Pinhead]
#305977 - 04/30/01 06:43 PM (21 years, 2 months ago) |
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ohhh ... interesting
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sparks
addict
Registered: 03/01/01
Posts: 55
Last seen: 21 years, 25 days
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Re: Agar/Petri dish prep. [Re: Triple_3]
#307326 - 05/02/01 06:56 AM (21 years, 2 months ago) |
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I usually make a lot more agar than i need at a time.
I pour it in small bottles, just enough to make 8-10 plates.
Then sterilise 30 min. and store them in the fridge.
They stay nice and clean for monts and months.
When needed i just nuke them until melted and pour my plates and sterilise them for 20 min.
Much easier than making new agar as soon as i need some plates.
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egghead
veteran
Registered: 04/25/01
Posts: 1,054
Loc: Milky Way
Last seen: 20 years, 3 months
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Re: Agar/Petri dish prep. [Re: sparks]
#307332 - 05/02/01 07:01 AM (21 years, 2 months ago) |
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Does that help you get things done more quickly?
I like to prepare extras so they're ready when I am.
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Where there's skill, there's a better way..
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Pinhead
Oregano
Registered: 05/14/00
Posts: 1,819
Loc: Hootersville
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Re: Agar/Petri dish prep. [Re: egghead]
#307408 - 05/02/01 10:35 AM (21 years, 2 months ago) |
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Its not so much that its quicker,but I usually only do 3-4 plates at a time. Its a pain in the ass to get out the scales,agar,etc and then do the math to make 1/4 - 1/2 a batch of agar at a time everytime I want to grow a culture.
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kingkc
enthusiast
Registered: 04/25/00
Posts: 1,064
Last seen: 4 years, 2 months
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Re: Agar/Petri dish prep. [Re: Pinhead]
#307564 - 05/02/01 03:09 PM (21 years, 1 month ago) |
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Great idea. I've heard of microwaving and pourng premade mix. Seems 5 min. in the microwave does the trick.
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