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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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Sterilising petri dishes?
    #816412 -

Hey everyone,
Just a quick question here. A couple of days ago I poured 9 petri dishes with some agar and it didn't set - (oops - still experimenting with ratios) anyway, I only have like 15 clean petris left so I was wondering what is the best way of sterilising these for re-use? They are the standard plastic kind. Alcohol wipe? Boiling?

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Re: Sterilising petri dishes? [Re: Peace_Patrol]
    #816414 -

Never tried to work with used plastic dishes. If it were me, I would throw them away.


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"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five

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Re: Sterilising petri dishes? [Re: Peace_Patrol] * 1
    #816417 -

They are not ment to be reused.
But IF, I?d recomend you to wash them thorouhghly with bleach water, let dry and wipe with alcohol or peroxide.


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Grow Mushrooms from Spores - PF-Tek for Simple Minds

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Re: Sterilising petri dishes? [Re: Anno]
    #816441 -

I'm not doubting your advice here Anno. I'm just curious to know if you've tried this in the past and what the failure rate was.


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"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five

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Re: Sterilising petri dishes? [Re: mickey_rourke]
    #816454 -

I tried this, yes. but only on 5 dishes.
I was using a flow hood and 1 dish contaminated.
I used iso-propanol, 70% to wipe the dishes.


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Grow Mushrooms from Spores - PF-Tek for Simple Minds

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Re: Sterilising petri dishes? [Re: Anno]
    #816463 -

Thanks for the advice guys. I'll give it a try. Otherwise I'll just get some more petri dishes. It's not that difficult, it just involves acting like I'm doing something legitimate to some scientists....or is there an easier way of getting petris?

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Re: Sterilising petri dishes? [Re: Peace_Patrol]
    #816481 -

In reply to:
It's not that difficult, it just involves acting like I'm doing something legitimate to some scientists....or is there an easier way of getting petris?

I just mail order the reusable glass kind. If you can't do this and you don't want to have to acquire more locally, you have alternatives. Before I had access to petri dishes, I would use 1/2 pint jars (the standard PF jar) with a bit of agar in the bottom. Just PC the jars after adding the agar to sterilize them.

Another alternative would be to tell the scientists who give you the dishes that you are cultivating oyster mushrooms and need to play with agar so that you can create spawn.


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"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five

Edited by mickey_rourke (08/13/02 11:38 PM)

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Re: Sterilising petri dishes? [Re: mickey_rourke]
    #816547 -

"Another alternative would be to tell the scientists who give you the dishes that you are cultivating oyster mushrooms and need to play with agar so that you can create spawn. "
I think they would know whats up, althogh that might only mean that you are parting with a little of your results


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If I made affront, I apologize.
If I made affirmation, I apologize.
I merely came to listen, came to say.

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Re: Sterilising petri dishes? [Re: Peace_Patrol]
    #817487 -

why bother ther 4-5 euro for twenty or buy a box anter like 2-3 for twenty and yes you grow mushrooms sow what thats legaly bistores can only be started that way ask for pda while your at it its cheap and good quality


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the above is what the litel leprecans told me to say and should be regarded as such
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Re: Sterilising petri dishes? [Re: DinoMyc]
    #818485 -

- Post History Deleted Upon User's Request -

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Re: Sterilising petri dishes? [Re: Peace_Patrol] * 1
    #819976 -

please don't take this to be a snyde remark of a jackass, but has it occured to anyone that there isn't a huge differance between a petri-dish and a wide mouth half-pint mason jar?

i use them with resealable plastic lids for many purposes like small culture stuff, storage of spore-print taken directly inside the jar, etc. both the jars and the lids are pc safe.

the biggest advantage of using an ordinary jar is that when you are done, you can still use the jar for other things, while a petri dish can only be used as a petri-dish. what's more, the plastic lids do a reasonable job of sealing against outside airborne contams, petridishes have to be taped.

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Re: Sterilising petri dishes? [Re: Peace_Patrol]
    #821527 -

I've had success re-using polystyrene petris by washing with soap and water then boiling for 5 mins. But then, I use peroxidated agar all the time, so sterility isn't so important.


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hmmm........

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Re: Sterilising petri dishes? [Re: Champion des Champignons]
    #821927 -

What would happen if you had a half pint w. agar in the bottom and u layed a fresh cap on top of the agar and let all the spores fall onto it, that would be huge overkill huh?

Vis


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"15 minutes in freezing conditions?!?! You'd become a popsicle before you made that!!!"

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Re: Sterilising petri dishes? [Re: tchyted]
    #823498 -

In reply to:
but has it occured to anyone that there isn't a huge differance between a petri-dish and a wide mouth half-pint mason jar?

I'd rather peer into a petri dish when looking at the contents as opposed to trying to look into a jar which has an opaque lid and must be viewed from an acute angle.


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"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five

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Re: Sterilising petri dishes? [Re: Visigoth]
    #823501 -

In reply to:
What would happen if you had a half pint w. agar in the bottom and u layed a fresh cap on top of the agar and let all the spores fall onto it, that would be huge overkill huh?

You will get a crash course in what your local contaminants are.


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"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five

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Re: Sterilising petri dishes? [Re: mickey_rourke]
    #824803 -

Mickey right. lol if you want to clone, just take a peice of your mushroom (the one that looks the biggest preferably) and mash it with a spoon and some (not a lot) peroxide. put in your jar and you will have a nice little culture.

entheo


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This is for the sole purpose of information only. Not intended for use in the actual manufacture of known drugs.

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Re: Sterilising petri dishes? [Re: entheo_smurf]
    #826650 -

In reply to:
Mickey right. lol if you want to clone, just take a peice of your mushroom (the one that looks the biggest preferably) and mash it with a spoon and some (not a lot) peroxide. put in your jar and you will have a nice little culture.


Uhm, how is your post relevant to the question he asked? I believe he was asking about multi-spore innoculation of agar and not cloning. Please read his question again for clarity if you don't understand:
In reply to:
What would happen if you had a half pint w. agar in the bottom and u layed a fresh cap on top of the agar and let all the spores fall onto it


Peace


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"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five

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Re: Sterilising petri dishes? [Re: mickey_rourke]
    #828596 -

I figured why multi-spore when you can just clone a fruit you liked. I am sorry if this rubbed you the wrong way man, but I would rather do that than multispore. Figured I could suggest something. More even flushing, no wondering about what kind of fruits you will get, etc..

entheo


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This is for the sole purpose of information only. Not intended for use in the actual manufacture of known drugs.

Edited by entheo_smurf (08/20/02 04:05 AM)

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Re: Sterilising petri dishes? [Re: entheo_smurf]
    #830890 -

Not too mention that mushrooms in general seem to senesces relatively rapidly from cloning too often. I am sure you could clone for a few crops, but after that you will probably start noticing strain degeneration. With multi-spore work you have the advantage of genetic diversity. I think both are good, but I consider clone work to be more of a temporary way to simply things. (In terms of homogeneity of the fruit.
As far as petri dishes go... I recommend getting glass ones. Environmentally friendly, re-usable, autoclavable... wonderful


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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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