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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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cellulosic ethanol and fungus enzymes
    #12612928 -

Hi guys !!
For the past few weeks i can not move my brain away from this subject. I have dug deep into this and have come up with some theories and questions that you might be able to help with.

Its a well known fact that fungi secrete enzymes onto cellulose based plant materials creating simple sugar chains via hydrolysis from the enzymes. I started thinking about grain jars that mycologist are very familiar with. When these jars are sterilized im thinking there are amylase enzymes inside the seeds that are being released as the heat gets turned up, releasing the amylase enzyme and allowing the grains to turn from starch into simple sugars. Also sterilizing and creating the breeding ground for fungus germination. Agar mixes are always made from simple sugars. And obviously the best choice for germination.

Once alive and well the fungus can digest a wide array of plant materials. Releasing cellulase enzymes to convert the cellulose into simple sugars to digest as it colonizes. This is a link ive been studying. That has experimentation with  Trichoderma reesei and Aspergillus niger.

Aspergillus niger is used in the production of high fructose corn syrup and also considered safe by the fda. The fungus is used to convert glucose into fructose during fermentation.

Is the fungus killed here to extract fructose? Is the mycelium a simple sugar?

This has been stirring in my head, so i wanted to type it out and get some other responses. Or some first hand accounts. I am going to do some experimentation on shredded copy paper very soon to see if i can make it practical. Too see if i can actually get some ethanol produced from the fungus conversion into simple sugars.

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Re: cellulosic ethanol and fungus enzymes [Re: Tomandjerry58]
    #12632420 -

I wanted to import some pictures from the U.S. Department of agriculture. forty-six different strains. These were the only"aerial view"style pics i have found from T.reesii on the internet, the highest producing strain of cullase.

T.reesii
Stromata of H. jecorina, the teleomorph of T. reesei, form on bark. They are light brown and ostiolar openings are visible."The fruits on the fourth pic" link


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Re: cellulosic ethanol and fungus enzymes [Re: Tomandjerry58]
    #12633015 -

> Is the fungus killed here to extract fructose?

Yes.

> Is the mycelium a simple sugar?

Myc is mostly made of chitin.


-FF

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Re: cellulosic ethanol and fungus enzymes [Re: fastfred]
    #12634261 -

I don understand what is your that you are trying to find out

??????


--------------------
eVenom

"Some of us like a nice buzz, while others want to climb a screaming volcano naked to meet god."
RR

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Re: cellulosic ethanol and fungus enzymes [Re: fastfred]
    #12636545 -

eVenom said:
I don understand what is your that you are trying to find out

??????


Cellulose is one of the most abundant materials on this planet. Trees,cotton,paper,grass..... 1000's of different materials are bound together by cellulose. Not to mention the products manufactured with cellulose that fill our landfills.

Fungi as we all know can live and eat all of these products of the environment and the manufactured world. Due to the enzymes they produce to systematically break down these cellulose chains. For the fungus to consume.

Just like bacteria in our stomachs produce enzymes to break down complex carbohydrates.Or just like cows have enzymes to break down cellulose to simple sugars. The circle of life if you will. My main goal is just to have a discussion about the subject with people that might have some insight. And of course put some experimentation to work for making ethanol from cellulase enzymes extracted from a fungus.



fastfred said:
> Is the fungus killed here to extract fructose?

Yes.

> Is the mycelium a simple sugar?

Myc is mostly made of chitin.


-FF


thanks.....chitin looks like a great byproduct that could be used for many different areas of agriculture.

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Re: cellulosic ethanol and fungus enzymes [Re: Tomandjerry58]
    #12637728 -

You might like to hear about quorn.

http://en.wikipedia.org/wiki/Quorn

It's a good story of industrial fungal research, which produced a viable product.


-FF

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Re: cellulosic ethanol and fungus enzymes [Re: fastfred]
    #12641946 -

Paul Stamets makes ethanol from oyster mushrooms he talks about it in mycelium running how mushrooms can help save the world


--------------------
eVenom

"Some of us like a nice buzz, while others want to climb a screaming volcano naked to meet god."
RR

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Re: cellulosic ethanol and fungus enzymes [Re: Tomandjerry58]
    #12681840 -

Quote:
Just like bacteria in our stomachs produce enzymes to break down complex carbohydrates.Or just like cows have enzymes to break down cellulose to simple sugars.


Termites are good at this.  Ever thought about extracting termite-gut bacteria and growing it outside the termites?  OT, yes...:alientransform:

Peace
-PS


--------------------

if you stand too close to the machine it'll start to eat you
Primal's simple tested teks and projects: :awesomenod: Wheat Prep 2.0  Acidic Tea Tek  Potency Project! 

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Re: cellulosic ethanol and fungus enzymes [Re: PrimalSoup]
    #12683237 -

PrimalSoup said:
Quote:
Just like bacteria in our stomachs produce enzymes to break down complex carbohydrates.Or just like cows have enzymes to break down cellulose to simple sugars.


Termites are good at this.  Ever thought about extracting termite-gut bacteria and growing it outside the termites?  OT, yes...:alientransform:

Peace
-PS


I wonder if the bacteria could be grown outside of the body. It seems that it would be best suited to be grown then extracted.

Here's some interesting info i found on wiki from the us department of energy.
link
Termites as a source of power
The US Department of Energy is researching ways to replace fossil fuels with renewable sources of cleaner energy, and termites are considered a possible way to reach this goal through metagenomics.[8]
Termites may produce up to two litres of hydrogen from digesting a single sheet of paper, making them one of the planet's most efficient bioreactors.[9] Termites achieve this high degree of efficiency by exploiting the metabolic capabilities of about 200 different species of microbes that inhabit their hindguts. The microbial community in the termite gut efficiently manufactures large quantities of hydrogen; the complex lignocellulose polymers within wood are broken down into simple sugars by fermenting bacteria in the termite's gut, using enzymes that produce hydrogen as a byproduct. A second wave of bacteria uses the simple sugars and hydrogen to make the acetate the termite requires for energy. By sequencing the termite's microbial community, the DOE hopes to get a better understanding of these biochemical pathways. If it can be determined which enzymes are used to create hydrogen, and which genes produce them, this process could potentially be scaled up with bioreactors to generate hydrogen from woody biomass, such as poplar, in commercial quantities.
Skeptics regard this as unlikely to become a carbon-neutral commercial process due to the energy inputs required to maintain the system. For decades, researchers have sought to house termites on a commercial scale (like worm farms) to break down woody debris and paper, but funding has been scarce and the problems of developing a continuous process that does not disrupt the termites' homeostasis have not been overcome.[10]

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Re: cellulosic ethanol and fungus enzymes [Re: Tomandjerry58]
    #12685246 -

:what2:

Interesting...

Peace
-PS


--------------------

if you stand too close to the machine it'll start to eat you
Primal's simple tested teks and projects: :awesomenod: Wheat Prep 2.0  Acidic Tea Tek  Potency Project! 

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