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The main roadblock is the woodchips or whatever you are using must be broken down chemically to free up the sugars so they can ferment.

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English Title:  Detection and recovery of hydrolytic enzymes from spent compost of four mushroom species.
Personal Authors: Ko HanGyu, Park SangHo, Kim SeongHwan, Park HyukGu, Park WonMok
Author Affiliation: School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea Republic.
Editors: No editors
Document Title : Folia Microbiologica,  2005  (Vol. 50)  (No. 2) 103-106

Abstract:

To evaluate the potential of using the enzymes from spent mushroom compost (SMC) as an industrial enzyme, the production of α-amylase, cellulase, β-glucosidase, laccase, and xylanase was determined from the SMC of four edible mushroom species (Pleurotus ostreatus, Lentinula edodes, Flammulina velutipes and Hericium erinaceum). Among the tested SMC, the SMC of L. edodes showed the highest enzyme activity in α-amylase (229 nkat/g), cellulase (759 nkat/g) and β-glucosidase (767 nkat/g) in 0.5% Triton X-100, and that of P. ostreatus showed the highest activity in laccase (1452 nkat/g) in phosphate-buffered 0.2% Triton X-100. The highest xylanase activity (119 nkat/g) was found in the SMC of F. velutipes.

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Publication number: CN101085994
Publication date: 2007-12-12
Inventor: BAILIANG ZHANG (CN); ANDONG SONG (CN)
Applicant: HENAN AGRICULTURAL UNIVERSITY (CN)
Classification:
- international: C12P7/10; C12P7/02;
- European:
Application number: CN20061017904 20060606
Priority number(s): CN20061017904 20060606


Abstract of CN101085994
The invention relates to a method for preparing fuel ethanol by taking straw as raw material. It comprises following steps: (1) selecting crop straw as raw material; (2) disintegrating: disintegrating straw by using hammer mill, sifting and getting straw powder of 20- 40 order; (3) pre- treating: lignin degrading raw material by way of culturing white-rot fungi in solid culture medium and liquid enzyme; (4) saccharifying two enzymes, saccharifying straw by using cellulase and xylanase at 45 Deg. C, pH 4.5, 150 rmp and ratio between liquid and solid 15, getting reduced sugar; (5) fermenting; (6) distilling. The fuel ethanol productivity is 16.7% in this invention, that is 16.7 ton waterless ethanol with every 100 ton straw, the production efficiency is high and economic benefit and social benefit is pretty good. Compared with current process, this invention is characterized in that the fermentation is thorough and the fuel ethanol production cost is low.

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ethanol is far more versatile since there is such a great range of feedstocks one drawback however is energy inputsd but that can be corrected by using a vacuum in the distillation process. we have made ethanol on a production level of 15 gallons per hour since 1978. the following are feedstocks we have used... gatoraid powder from quaker oats at a rate of seven tons per week that would otherwwise been landfilled, rice cakes from the same souyrce at the rate of seven tons per week that otherwise would have been landfilled, apple cider pulp at a rate of 3000 lbs per week that would otherwise been landfilled, wind fallen fruit from orchards at a rate of six tons per week that would have otherwise rotted on the ground most recently we made ethanol from wastes from debbies cakes not only did we make ethanol but we also made biodiesel from the very same source, we made ethanol from culled carrots at a rate of seven tons per week, that would have otherwise been improperly disp[osed of saving a major carrot grower thousands in fines from the EPA. we made ethanol from sulphite liquors from papermills. ,from flat beer collected from bars and microbtreweries, from flat cocacola, ginger ale, and the wash off from cocacola bottling companies which contain 5% sugars this use of waste streams is largely ignored as a source of fuel.. ecogenics has been at the forfront of producing fuel from weaste for thirty years. we have made ethanol from rice straw, using cellulose hydrolysis. and the list of alternative crops is endless. ethanol will be at the forfront of alternatyive fuels if waste streams are used for the feed stocks just like we have shown that it is economically viable... see our website for more on ethanol from wastes marc Marc Orion Cardoso www.ecogenicsresearchcenter.org

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