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whiteshadow
Stranger
Registered: 12/02/09
Posts: 36
Last seen: 13 years, 10 months
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does clone+agar= monoculture?
#11634957 - 12/12/09 08:26 AM (14 years, 2 months ago) |
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This may be a stupid question, but if one grows out a clone on agar, is that agar culture considered a monoculture, or is further isolation necessary?
-------------------- Never order from university scientific, not even in a jam...they will rip you off.
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RonPaulVerm
Piss Christ


Registered: 07/21/08
Posts: 1,072
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Re: does clone+agar= monoculture? [Re: whiteshadow]
#11636243 - 12/12/09 12:36 PM (14 years, 2 months ago) |
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cloning a clone? is that what you are speaking of? A flush of clones is a monoculture in my book.
-------------------- Spawn I Have: Blewitt, Reishi, Maitake, Chicken of the Woods, Parasol, Shaggy Mane, King, Blue and Gold Oyster, Shiitake, King Stropharia, Lions Mane, Almond Portabello, Elm Oyster, Phoenix Oyster, Nameko, Enoki, White Beech, Spawn I Want: Corcyceps Sp., Cauliflower Mushrooms, Agaricus species (Portobello), Pink Oyster, Piopinno, ...anything else you might have Primitive Skills Any information I've posted is only related to fictional purposes. I do not advocate growing any illegal mushrooms ...I DO advocate learning about Law Enforcement Against Prohibition
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Kamek


Registered: 01/08/05
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Re: does clone+agar= monoculture? [Re: RonPaulVerm]
#11637180 - 12/12/09 02:56 PM (14 years, 2 months ago) |
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clone (kln) n. 1. A cell, group of cells, or organism that is descended from and genetically identical to a single common ancestor, such as a bacterial colony whose members arose from a single original cell. 2. An organism descended asexually from a single ancestor, such as a plant produced by layering or a polyp produced by budding. 3. A DNA sequence, such as a gene, that is transferred from one organism to another and replicated by genetic engineering techniques. 4. One that copies or closely resembles another, as in appearance or function: "filled with business-school clones in gray and blue suits" (Michael M. Thomas). v. cloned, clon·ing, clones v.tr. 1. To make multiple identical copies of (a DNA sequence). 2. To create or propagate (an organism) from a clone cell: clone a sheep. 3. To reproduce or propagate asexually: clone a plant variety. 4. To produce a copy of; imitate closely: "The look has been cloned into cliché" (Cathleen McGuigan). v.intr. To grow as a clone.
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PitcherCrab
Crescent Fresh



Registered: 12/02/06
Posts: 2,446
Loc: The bottom of the sea.
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Re: does clone+agar= monoculture? [Re: RonPaulVerm]
#11637189 - 12/12/09 02:57 PM (14 years, 2 months ago) |
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It depends on if the culture was an isolate to begin with. Mushrooms can grow from from mycelium that has more than one culture in it so if you clone from one of those mushrooms you can still isolate it out into a monoculture once its been cloned to agar. Commercial growers only use isolates so if you clone from a store bought mushroom you will be getting a an isolated culture. That is my understanding of it anyway... search for cloning vs. isolating. There is quite a bit of information on the difference.
-------------------- PC's LAGM 2021 (TOC & TWC)P. natalensis Growlog 2021Pans for PC Fall 2021 Growlog “I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear
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