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Offlinewhiteshadow
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Registered: 12/02/09
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Last seen: 13 years, 10 months
does clone+agar= monoculture?
    #11634957 - 12/12/09 08:26 AM (14 years, 2 months ago)

This may be a stupid question, but if one grows out a clone on agar, is that agar culture considered a monoculture, or is further isolation necessary?


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InvisibleRonPaulVerm
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Re: does clone+agar= monoculture? [Re: whiteshadow]
    #11636243 - 12/12/09 12:36 PM (14 years, 2 months ago)

cloning a clone? is that what you are speaking of?
A flush of clones is a monoculture in my book.


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OfflineKamek
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Re: does clone+agar= monoculture? [Re: RonPaulVerm]
    #11637180 - 12/12/09 02:56 PM (14 years, 2 months ago)

clone  (kln)
n.
1. A cell, group of cells, or organism that is descended from and genetically identical to a single common ancestor, such as a bacterial colony whose members arose from a single original cell.
2. An organism descended asexually from a single ancestor, such as a plant produced by layering or a polyp produced by budding.
3. A DNA sequence, such as a gene, that is transferred from one organism to another and replicated by genetic engineering techniques.
4. One that copies or closely resembles another, as in appearance or function: "filled with business-school clones in gray and blue suits" (Michael M. Thomas).
v. cloned, clon·ing, clones
v.tr.
1. To make multiple identical copies of (a DNA sequence).
2. To create or propagate (an organism) from a clone cell: clone a sheep.
3. To reproduce or propagate asexually: clone a plant variety.
4. To produce a copy of; imitate closely: "The look has been cloned into cliché" (Cathleen McGuigan).
v.intr.
To grow as a clone.


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OfflinePitcherCrab
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Re: does clone+agar= monoculture? [Re: RonPaulVerm]
    #11637189 - 12/12/09 02:57 PM (14 years, 2 months ago)

It depends on if the culture was an isolate to begin with. Mushrooms can grow from from mycelium that has more than one culture in it so if you clone from one of those mushrooms you can still isolate it out into a monoculture once its been cloned to agar. Commercial growers only use isolates so if you clone from a store bought mushroom you will be getting a an isolated culture.
That is my understanding of it anyway... search for cloning vs. isolating. There is quite a bit of information on the difference.


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