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OfflineAmericaOnLSD
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Cloning from Store-Bought Mushroms
    #6273144 - 11/11/06 06:50 PM (17 years, 2 months ago)

Okay, so I've decided to try my hand a growing gourmet mushrooms to get some experience before deciding whether or not to risk growing other types of mushrooms. Being the type who enjoys making do with what I have vs. running out and buying a whole bunch of stuff, I did the following after a bit of reading:
1. I bought some fresh mushrooms while grocery shopping. I bought one container of store brand "organically grown - no preservatives" mushrooms (I assume these are buttons) and one of fresh Shiitake mushrooms.
2. I made liquid culture solution using a tablespoonful of dry malt extract (which I already had from my brewing hobby) per 8oz of water in canning jars. After covering the jars with two layers of aluminum foil, I boiled them for one hour in a large covered pot.
3. After allowing the jars to cool completely, I prepared the mushrooms. I showered, then rinsed my hands in rubbing alcohol. I wiped a large dish with rubbing alcohol and put one of each type of mushroom in the dish. I then wiped the mushrooms themselves with alcohol. Using a regular kitchen knife (which I also wiped with alcohol), I cut several pieces from the inside of the mushroom stems. I lifted the top layer of foil, poked a hold in the bottom layer with the knife, and dropped several match-head sized pieces of the two types of mushroom into the two jars.

I did this 5 days ago. Now my jars look as follows. The one I put the button mushrooms in has what looks like ground up mushroom powder accumulating in a thin layer on the bottom of the jar (especially around the edges). Some of this mushroom matter is also floating in the jar within about an inch of the bottom. The matter looks brown, although that may be due to the solution being brown due to the malt extract. Where the mushroom powder touches the bottom of the jar, it looks between brown and white. The jar I put the shiitake mushrooms in has a cloudy layer within about an inch of the bottom. When I swirl it, it looks like smoke. There is also some powder similar to that in the button mushroom jar settling to the bottom.

Does it sound from this description like I am successfully growing mushroom cultures, or would this be contamination? I had kind of expected that the mushroom growth would be attached to the pieces of mushrooms but this is not what I'm seeing; it appears that the "mushroom powder" is growing by itself separate from the parent mushroom pieces.

How long should it take to fill the jar with culture? The room temperature averages around 70F.

If I am successful with culturing, can I just squirt some of the resulting solution on sterilized sawdust and expect mushrooms to grow?

Thanks for any help.


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Invisiblepoboy
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Re: Cloning from Store-Bought Mushroms *DELETED* [Re: AmericaOnLSD]
    #6273533 - 11/11/06 09:22 PM (17 years, 2 months ago)

Post deleted by poboy

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Edited by poboy (11/11/06 09:45 PM)


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Offlinechrisscole
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Re: Cloning from Store-Bought Mushroms [Re: poboy]
    #6305066 - 11/27/06 04:08 PM (17 years, 2 months ago)

thought i'd add to this thread rather than start up a new one.

I started some agar cultures from store bought clones about a month ago, and have found that oysters worked fine, but everything else just went brown and had no mycelial growth.  I also got one of those agaricus kits, and tried cloning some tissue from the inside of the stalk (broken open so as not to introduce contams) and that has just turned brown and doesn't look like it has any myc growth.

Any thoughts as to why this is?  Wondering if maybe I should imbed the tissue in the agar or something, so it's not open to the air - or maybe put some peroxide in with it.

As a flip side, just inoculated my first bags of straw with home grown blue oyster spawn...pretty excited (oh the joys of being a newbie :smile: ) I've got a bag of sawdust/woodchips/bran, one of straw, one of straw and newspaper and one newspaper all growing now, will send some pics if I manage to fruit them.

cheers,
Chris


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OfflineAmericaOnLSD
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Re: Cloning from Store-Bought Mushroms [Re: chrisscole]
    #6305362 - 11/27/06 05:10 PM (17 years, 2 months ago)

Update on mine. The Shiitake mushrooms apparently did nothing - I was just seeing bits of malt extract floating around. The button mushroom did grow some fuzz near it in the liquid, but it stopped growing in a few days. When I later removed the aluminum foil cover I found a green mold growing on the top so I threw it away.

I did however buy two mushroom logs from a commercial supplier (one Shiitake, one Blue Oyster). I have a few buds on the Shiitake kit (started 10 days ago).


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OfflineRogerRabbitM
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Re: Cloning from Store-Bought Mushroms [Re: AmericaOnLSD]
    #6307214 - 11/28/06 10:16 AM (17 years, 2 months ago)

Commercial mushroom farms tend to grow out their mycelium to the point of senescence prior to fruiting, so clones taken from the grocery store usually perform poorly if at all.
RR


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Offlinechrisscole
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Re: Cloning from Store-Bought Mushroms [Re: RogerRabbit]
    #6307238 - 11/28/06 10:24 AM (17 years, 2 months ago)

that would explain it.

did have some success with the oysters though, got a batch of agar, though I can't remember which jars of spawn I inoculated with it.

AmericaonLSD- good luck with the grows.


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Offlinechrisscole
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Re: Cloning from Store-Bought Mushroms [Re: AmericaOnLSD]
    #6307243 - 11/28/06 10:27 AM (17 years, 2 months ago)

Quote:

AmericaOnLSD said:
I did however buy two mushroom logs from a commercial supplier (one Shiitake, one Blue Oyster). I have a few buds on the Shiitake kit (started 10 days ago).




yeah, got a shiitake log myself a while back. had one mushroom from it so far, and the other log nothing.


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OfflineAmericaOnLSD
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Re: Cloning from Store-Bought Mushroms [Re: chrisscole]
    #6322155 - 11/30/06 05:38 PM (17 years, 2 months ago)

Yeah I've got one mushroom too right now (Shiitake). I think it is a bit early - the log has not even turned brown yet. Note: I removed the plastic tent I usually keep over the log in order to take the picture.



I will take a spore print and/or tissue culture once it matures and then try growing my own.


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Invisiblepoboy
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Re: Cloning from Store-Bought Mushroms *DELETED* [Re: RogerRabbit]
    #6322848 - 11/30/06 09:05 PM (17 years, 2 months ago)

Post deleted by poboy

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Offlinechrisscole
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Re: Cloning from Store-Bought Mushroms [Re: poboy]
    #6323892 - 12/01/06 10:12 AM (17 years, 1 month ago)

yeah,

got quite a good plate from the oysters I bought from the supermarket, absolutely nothing from the portobellos and buttons though. They look nice...mmm.

Chris


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OfflineAmericaOnLSD
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Re: Cloning from Store-Bought Mushroms [Re: chrisscole]
    #6333728 - 12/04/06 05:52 PM (17 years, 1 month ago)

My Shiitake mushroom ended up being about 3 inches in diameter. I harvested it when I noticed the plastic bag turning white from spores. The mushroom weighed 2.1 ounces. I let this sit on aluminum foil for about 12 hours with a dish over top of it and the foil turned white under the mushroom - except near the center where some water condensed. I folded the foil over into somewhat of a tube shape to try to keep out dust, then let this sit for half a day until it dried out. I then folded the foil shut and labeled. I have a 2nd piece of foil doing a 2nd print right now.

I bought some potato dextrose agar and petri dishes. After sterilizing the area and my hands with Lysol I tore the stem of the mushroom apart and placed a small piece in 2 agar dishes. I also put some spores from the foil (picked up with the prongs of a fork wiped with rubbing alcohol) in 2 other agar dishes. I am keeping the dishes around 75 degrees. About how long should it take for them to show growth?

Also I don't see any more mushrooms forming on the Shiitake log. Most of the log is white, with some brown patches. Should I continue to keep the log moist or should I consider the one mushroom I got to be a "flush" and follow the directions about letting the log dry for a week and then soaking in cold water?


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OfflineCryogenicz
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Re: Cloning from Store-Bought Mushroms [Re: AmericaOnLSD]
    #6338812 - 12/05/06 10:47 PM (17 years, 1 month ago)

I also have cloned from Store bought Oyster, and they worked well. But, the white button clone didnt do so well.. very whispy. that was 2 years ago.. never attempted after that.


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OfflineAmericaOnLSD
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Next Steps - Shiitake Clone [Re: AmericaOnLSD]
    #6371762 - 12/14/06 08:19 PM (17 years, 1 month ago)

I managed to clone my Shiitake onto PDA agar. It has been 10 days and now most of the agar (10cm diameter plate) is covered with mycelium. I don't see any signs of contamination. I did see a couple of dots start growing away from the main mycelium but it is white and looks identical to the main mycelium so I'm assuming some little pieces must have broke off the main area when I pick up the plates to look at them. I have 2 plates of cloned, plus I did two plates of spores. The spores are growing more slowly, and have only spread about 1/2 inch from the center of where they were placed. I don't see any contams - I do see a few dots of mycelium elsewhere on the plates but I assume a few spores could have easily landed there when I placed the spores in the center.

I believe the next step I should take is agar to grain, correct? I'm planning on sterilizing some popcorn in a PC using a tek from this site and will then do agar to grain. I will wait until the spores grow a bit more and then plan to inoculate 2 jars of grain on Sunday one with the mycelium from the clone and one with the mycelium from the spores. Will put the other 2 agar plates in the refrigerator so I will have them to try again if something goes wrong. I seem to remember reading that mycelium from the Shiitake spores will not fruit very well, is this true? I will experiment anyway.

I then let the popcorn colonize and spawn to wood, right? If possible, I would like to stick to things I can get at the grocery and pet store. Could I use a mixture of aspen pet bedding and brown rice flower made by grinding up long-grain brown rice when I get to that point?


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Edited by AmericaOnLSD (12/14/06 08:22 PM)


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Offlinefalcon
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Re: Next Steps - Shiitake Clone [Re: AmericaOnLSD]
    #6372370 - 12/14/06 11:06 PM (17 years, 1 month ago)

Could I use a mixture of aspen pet bedding and brown rice flower made by grinding up long-grain brown rice when I get to that point?

Yes.

Oat bran and wheat bran will also work and would not have to be ground.


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OfflineLentinulaEdodes
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Re: Next Steps - Shiitake Clone [Re: falcon]
    #6390919 - 12/20/06 03:49 PM (17 years, 1 month ago)

^Agreed


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OfflineAmericaOnLSD
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Re: Next Steps - Shiitake Clone [Re: LentinulaEdodes]
    #6429169 - 01/05/07 08:00 PM (17 years, 26 days ago)

I am growing both cloned Shiitake and Shiitake spores in both popcorn and an 80% aspen pet bedding / 20% BRF mixture. Here are some pictures after a couple weeks.




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