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InvisibleSpeeker

Registered: 02/11/04
Posts: 664
Pinback's <Armillaria mellea on agar (pics)> thread
    #3251563 - 10/19/04 02:53 PM (13 years, 1 month ago)

Quote:

Pinback said:

Armillaria mellea sensu lato, with very evil-looking rhizomorphs growing under the surface of the agar. The mycelium is luminescent, but my camera couldn't capture it at F/2.8 for 15 secs.



Backlit:



In situ at the base of a dead birch (old picture):






Quote:

Suntzu said:
Awesome! What agar are you using? I tried cloning one recently, it doesn't seem to have taken to PDA or cornmeal agar.
The backlit shot is crazy. Looks like plant roots!
The inoculum looks almost purplish. . .is that normal?
Nice job!





Quote:

Pinback said:
Thanks! It is standard PDYA, but the "explant" wedge is PDYA + activated charcoal, for no reason other than that I had some previously poured plates around when I cloned it. The color is more like brown with a slight reddish tint in real life, but I have no idea if that is normal or not. I'd be glad to send you a wedge if you want one. I am sure you know it is/can be a parasite and will take proper caution.  :smile:





Quote:

Speeker said:
good looking "roots" it has!  :smile:

I just cloned A. borealis (I think, more common here).
it has not yet developed anything like those...
And its mycelium becomes yellowish-brown when it ages
instead of reddish. It is this older mycelium that glows dimly.





Quote:

ragadinks said:
Great pics !
I also have cloned Armillaria ostoyae 10 days ago and some of the clones have developed rhizomes very fast - have never seen that before.
But I could not see the mycelium glowing the dark - probably it is still too small ?

Here are some pics:




Does anybody know if someone has succeeded in cultivating Armillaria ?
I have read that they already have done it, but I have never seen an actual article about it on the net.




Quote:

Pinback said:
Nice, I especially like the second picture, top row!

To be able to see the glow, there has to be complete darkness, and the eyes have to adapt for a few minutes... at least with my eyes/mycelium. The amount of growth you have should be enough if your strain (or species) behaves as the one I have. I've also read that they can be cultivated, but not much details. I'll look into it tomorrow if I have time.




Quote:

Speeker said:

Quote:

ragadinks said:
But I could not see the mycelium glowing the dark - probably it is still too small ?




Either that or your weren't there (in the dark ) long enough. :wink:
It takes time for eyes to adapt enough .. also movement of the plate helps..





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Offlineragadinks
MrBeatle
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Registered: 10/20/03
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Re: Pinback's <Armillaria mellea on agar (pics)> thread [Re: Speeker]
    #3251786 - 10/19/04 03:49 PM (13 years, 1 month ago)

hey speeker you smart guy - how did you recover that posting :rolleyes: ?

> It takes time for eyes to adapt enough .. also movement of the plate helps..
Maybe my eyes were not adapted to the darkness well enough - will try it out again  ...


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InvisibleSpeeker

Registered: 02/11/04
Posts: 664
Re: Pinback's <Armillaria mellea on agar (pics)> thread [Re: ragadinks]
    #3252030 - 10/19/04 05:02 PM (13 years, 1 month ago)

from my browser's cache..


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InvisiblePinback
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Re: Pinback's <Armillaria mellea on agar (pics)> thread [Re: Speeker]
    #3255050 - 10/20/04 07:49 AM (13 years, 1 month ago)

Nice job saving the thread!

I went to a university library and looked in "The biology and cultivation of edible mushrooms" by Chang (1978), "The Fungi" by Sussman (1965-1973) and a thesis about Armillaria infection biology, but I didn't find anything about the cultivation, only that it had been done. I found some interesting things though: At least A. mellea sensu stricto doesn't seem too picky about the wood it is growing on. It has been found both on hardwood (most common) and softwood. A. ostoyae is more common on softwood, and is a more aggressive parasite than A. mellea. One of the conclusions of the thesis was that their primary mode of spreading is through vegetative growth, and not spores, which I think is good news for us.


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Offlineragadinks
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Re: Pinback's <Armillaria mellea on agar (pics)> thread [Re: Pinback]
    #3255088 - 10/20/04 08:39 AM (13 years, 1 month ago)

> but I didn't find anything about the cultivation, only that it had been done.
Same thing with me - also only could find some hints that it has been done, but not how.
Only once I have read an article where it said that they used oranges in the substrate to get them to pin !?
Maybe I can find this article again ...

> their primary mode of spreading is through vegetative growth, and not spores, which I think is good news for us.
Why is that better ?


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InvisiblePinback
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Re: Pinback's &lt;Armillaria mellea on agar (pics)&gt; thread [Re: ragadinks]
    #3255460 - 10/20/04 11:47 AM (13 years, 1 month ago)

I think it's better because that means the risk of spreading a tree pathogen, which Armillaria is, is smaller. Maybe I'm just overly cautious though, because the mushrooms is already there in nature, spreading its spores...


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OfflineMorrie
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Re: Pinback's <Armillaria mellea on agar (pics)> thread [Re: ragadinks]
    #22334823 - 10/04/15 11:15 PM (2 years, 1 month ago)

Quote:

ragadinks said:
> but I didn't find anything about the cultivation, only that it had been done.
Same thing with me - also only could find some hints that it has been done, but not how.
Only once I have read an article where it said that they used oranges in the substrate to get them to pin !?
Maybe I can find this article again ...

> their primary mode of spreading is through vegetative growth, and not spores, which I think is good news for us.
Why is that better ?




Cultivation has been done on a lab scale as an aid to genetic studies. The literature is old and not in English, mainly.  Use A. mellea sensu stricto or A. tabescens for the best results.  Others are harder to get fruiting.  Substrate choice is not critical.  I suggest shiitake type of substrate or grain/sawdust.  Incubate in the dark at 25C and then cold shock at 15C with ambient daylight.


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