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fractional sterilization success story! (well, not really)
    #11615490 -

well i made 4 half pint jars of WBS and did fractional sterilization. (steam 1 hour, wait a day, steam 1 hour, wait a day, steam one hour, wait until cool, inoculate) and i lost 3 of the jars early, however i've got one champion :grin: was at about 30 percent today so i shook it :thumbup:

fractional sterilization works.... just not very well... at all :cool:


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Edited by rsinatra13 (12/09/09 09:51 AM)

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Re: fractional sterilization success story! (so far) [Re: rsinatra13]
    #11615500 -

rsinatra13 said:
fractional sterilization works.... just not very well... at all :cool:



You've confirmed hundreds of years of research right in your own kitchen! :thumbup:


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You make it all possible. Doesn't it feel good?

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Re: fractional sterilization success story! (so far) [Re: Doc_T]
    #11615509 -

Doc_T said:
rsinatra13 said:
fractional sterilization works.... just not very well... at all :cool:



You've confirmed hundreds of years of research right in your own kitchen! :thumbup:



haha i went into it expecting to lose all of them :tongue:
nothing like experimenting though:vaped:


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Re: fractional sterilization success story! (so far) [Re: rsinatra13]
    #11615701 -

One must be very careful calling a 75% failure rate prior to full colonization a 'success story'.  With a pressure cooker, one can attain a 99.9% or better success rate.  I can think of no other tool of the trade which has such a high payoff.
RR


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"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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Re: fractional sterilization success story! (so far) [Re: RogerRabbit]
    #11615719 -

RogerRabbit said:
One must be very careful calling a 75% failure rate prior to full colonization a 'success story'.  With a pressure cooker, one can attain a 99.9% or better success rate.  I can think of no other tool of the trade which has such a high payoff.
RR



the "success story" was sort of meant to be sarcasm
and yes i know there's now replacement for a PC; however, sadly its not within my budget.
I'm aware 75% failure is not a success story. I was pumped that one jar made it through like a champ so i had to share :thumbup:


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Re: fractional sterilization success story! (so far) [Re: rsinatra13]
    #11615737 -

How did you rinse the grains? Just water? Or with a drop of soap?
I bet a good washing would get you up to 33%. :lol:


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You make it all possible. Doesn't it feel good?

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Re: fractional sterilization success story! (so far) [Re: Doc_T]
    #11615845 -

i just used water for the rinse :smirk:


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Re: fractional sterilization success story! (so far) [Re: rsinatra13]
    #11615945 -

Ok, then in all seriousness;
Rinse with soap next time, then use clear water until the soap is gone.
You might even pour in enough peroxide to cover them- not so much for killing things, but to fizz up and break the bacteria and spores loose so you can rinse them away cleanly.
The cleaner your initial product is, the better your results will be.

Also, go find a PC in a thrift store or get one for free from somebody.


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You make it all possible. Doesn't it feel good?

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Re: fractional sterilization success story! (so far) [Re: Doc_T]
    #11616030 -

Doc_T said:
Ok, then in all seriousness;
Rinse with soap next time, then use clear water until the soap is gone.
You might even pour in enough peroxide to cover them- not so much for killing things, but to fizz up and break the bacteria and spores loose so you can rinse them away cleanly.
The cleaner your initial product is, the better your results will be.

Also, go find a PC in a thrift store or get one for free from somebody.



yeahh there wont be a next time without a PC :wink:
and trust me i've been checking thrift stores, someday i'll get one


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Re: fractional sterilization success story! (well, not really) [Re: rsinatra13]
    #11634816 -

rsinatra13 said:
well i made 4 half pint jars of WBS and did fractional sterilization. (steam 1 hour, wait a day, steam 1 hour, wait a day, steam one hour, wait until cool, inoculate)



Not surprising that you failed using such short times. I have pointed out many times that the tek on this site for FS is utterly useless, it COMPLETELY misses the point that people think is obvious for PC'ing. Namely that it takes a long time for the core of grains in the middle of a jar to reach temp.

The tek is transcribed direct from textbooks, the very same textbooks would tell you to PC for maybe 15-20mins, but everybody knows & accepts that this is not long enough, but for some very odd reason they cannot apply the same logic to FS. Its no wonder FS gets such a bad name, if people followed textbook advice for PC's they would have a bad name too.

When PCing it takes time to reach pressure, and time to cool down from 15psi. Many peoples jars could be subjected to steam for longer than people are doing these half assed attempts of FS. i.e. it could take 20mins to reach 15psi, and 20mins to come down to 0psi, so exposed to 40mins at 100C+ yet people will steam jars of cold grain for 30min intervals, it beggars belief. 60mins was used above, not nearly enough. The additional PC'ing time is worked out empirically by people, i.e. with a normal PC and stove it is being heated for all that time before it reaches 15psi.

It pisses me off to see so much time & effort & grains wasted by half assed attempts. Then it further discourages people from trying it out.

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Re: fractional sterilization success story! (well, not really) [Re: blackout]
    #11635078 -

blackout said:
rsinatra13 said:
well i made 4 half pint jars of WBS and did fractional sterilization. (steam 1 hour, wait a day, steam 1 hour, wait a day, steam one hour, wait until cool, inoculate)



Not surprising that you failed using such short times. I have pointed out many times that the tek on this site for FS is utterly useless, it COMPLETELY misses the point that people think is obvious for PC'ing. Namely that it takes a long time for the core of grains in the middle of a jar to reach temp.

The tek is transcribed direct from textbooks, the very same textbooks would tell you to PC for maybe 15-20mins, but everybody knows & accepts that this is not long enough, but for some very odd reason they cannot apply the same logic to FS. Its no wonder FS gets such a bad name, if people followed textbook advice for PC's they would have a bad name too.

When PCing it takes time to reach pressure, and time to cool down from 15psi. Many peoples jars could be subjected to steam for longer than people are doing these half assed attempts of FS. i.e. it could take 20mins to reach 15psi, and 20mins to come down to 0psi, so exposed to 40mins at 100C+ yet people will steam jars of cold grain for 30min intervals, it beggars belief. 60mins was used above, not nearly enough. The additional PC'ing time is worked out empirically by people, i.e. with a normal PC and stove it is being heated for all that time before it reaches 15psi.

It pisses me off to see so much time & effort & grains wasted by half assed attempts. Then it further discourages people from trying it out.



interesting to note and thanks for the input. :thumbup:


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Re: fractional sterilization success story! (well, not really) [Re: rsinatra13]
    #11635111 -

instead of fractional sterilization, if you can't afford a PC, just sterilize your grains on venus. the psi there is like 1300 or something like that.


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Re: fractional sterilization success story! (well, not really) [Re: Citizen13]
    #11635185 -

Citizen13 said:
instead of fractional sterilization, if you can't afford a PC, just sterilize your grains on venus. the psi there is like 1300 or something like that.



i already tried that :stoned: no luck


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Re: fractional sterilization success story! (well, not really) [Re: rsinatra13]
    #11637443 -

A small PC can be had for $20 at walmart.  If you can't afford that then how are you affording all the other supplies which add up to well more than $20?


-FF

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Re: fractional sterilization success story! (well, not really) [Re: fastfred]
    #11639418 -

fastfred said:
A small PC can be had for $20 at walmart.  If you can't afford that then how are you affording all the other supplies which add up to well more than $20?


-FF



okay, TECHNICALLY i can afford a pc, i dont wanna waste my money, on a small walmart PC. i'd rather PF tek it until i can afford an AA or something


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Re: fractional sterilization success story! (well, not really) [Re: rsinatra13]
    #11643674 -

Even when you get into the hobby it's still nice to have a small PC for small loads.

It's a lot faster and cheaper to fire up a small PC and unless you're really good at planning you'll probably find lots of times you'll want to run a small fast load rather than wait for a huge PC just to do one syringe or a small tool.


-FF

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Re: fractional sterilization success story! (well, not really) [Re: rsinatra13]
    #11643745 -

Search amazon.com for presta pressure cookers. They are large and inexpensive, and guess what, work! I think the AA cookers are overkill for those not obsessed with the hobby.

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Re: fractional sterilization success story! (well, not really) [Re: SFsorrow]
    #11643855 -

2 questions. my extra pc is missing the little bobber for the top. my nice one doesnt even have one of those, so do i need it? also could i buy a psi guage and attatch that instead?

question number two- why cant you nuke a spawn substrate in the microwave for 30 minutes. i have a pc i was just messing around and it seems to be working fine


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Re: fractional sterilization success story! (well, not really) [Re: Wimy]
    #11644993 -

1) buy the jiggler.
2) because it won't work.


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You make it all possible. Doesn't it feel good?

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Re: fractional sterilization success story! (well, not really) [Re: Doc_T]
    #11645035 -

im not gonna spend any money on that old tiny pc. i want one with a guage built on it. a big one


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