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OfflineAeolus1369
Dr. Seahorse
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Registered: 05/20/02
Posts: 367
Last seen: 15 years, 1 month
Anyone have experience with fractional sterilization?
    #2452043 - 03/19/04 03:42 PM (20 years, 4 days ago)

Hey all--

After a 1 year hiatus, I'm thinking about getting a new project underway and wanted to give grains a try. I don't have access to a pressure cooker and as such, am wondering if anyone has experience with the fractional sterilization method outlined in the FAQ (or any other FS tek for that matter). Does this procedure require a modification of the typical grain recipes? What particular grains were used etc. Is it worth my time trying grains without a PC or should I just go back to BRF cakes? Also, because the grains are shaken to encourage rapid colonization, is it ok to use less liquid innoculant than one would with cakes?

Also, since I've never tried casing I was gonna give it a go regardless of whether I end up using BRF cakes or grain. Just ooc, though, is it possible to fruit off of "grain cakes?"

--Aeolus

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Offlinemockeylock
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Registered: 02/07/04
Posts: 1,046
Last seen: 13 years, 8 months
Re: Anyone have experience with fractional sterilization? [Re: Aeolus1369]
    #2452133 - 03/19/04 04:12 PM (20 years, 4 days ago)

The general concensus is taht pressure cookers are a must with grains. I wouldn't try it. As far as grain cakes are concerned, they don't have the moisture in them to support that kind of growth. If it were to fruit, the weight of the shroom would probably rip the grains it was attatched to right off. But try casing some PF cakes....it works wonders!


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Offlinemockeylock
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Re: Anyone have experience with fractional sterilization? [Re: Aeolus1369]
    #2452142 - 03/19/04 04:14 PM (20 years, 4 days ago)

And even though I haven't been around long enough to remember you:
:mushroom2: :mushroom2: :mushroom2:WELCOME BACK!!! :mushroom2: :mushroom2: :mushroom2:


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OfflineAeolus1369
Dr. Seahorse
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Registered: 05/20/02
Posts: 367
Last seen: 15 years, 1 month
Re: Anyone have experience with fractional sterilization? [Re: mockeylock]
    #2452455 - 03/19/04 05:50 PM (20 years, 4 days ago)

I know conventional wisdom says use a pressure cooker, however the fractional sterilization technique seems viable (based on my moderate knowledge of microbiology). Has anyone actually tested it though?

--Aeolus

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OfflineTinTree
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Registered: 02/15/04
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Re: Anyone have experience with fractional sterilization? [Re: Aeolus1369]
    #2455574 - 03/20/04 08:34 PM (20 years, 3 days ago)

things that go BUMP in the night
I'm interested in hearing about this too.


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"I'm afraid of losing my obscurity. Genuineness only thrives in the dark. Like celery."
- Aldous Huxley

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Invisiblesnatchcakes
Rad Dude

Registered: 01/17/04
Posts: 714
Loc: HellTrack
Re: Anyone have experience with fractional sterilization? [Re: Aeolus1369]
    #2455589 - 03/20/04 08:39 PM (20 years, 3 days ago)

I used it on rye grain heres a thread i started about it.I had absolutly 100% success rate with it.
http://www.shroomery.org/forums/showflat.php?Cat=&Board=Contams&Number=2252620&Forum=

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OfflineChiLong
More machine elfthan man...

Registered: 02/22/03
Posts: 165
Loc: Twisted, moved to E-Ville
Last seen: 18 years, 10 months
Re: Anyone have experience with fractional sterilization? [Re: Aeolus1369]
    #2456006 - 03/20/04 11:48 PM (20 years, 2 days ago)

100% failure on every attempt on popcorn.

Get a pressure cooker if you want to work with it.

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Anonymous

Re: Anyone have experience with fractional sterilization? [Re: Aeolus1369]
    #2456038 - 03/21/04 12:07 AM (20 years, 2 days ago)

don't want to sound like a broken record but...
pressur cooker man. get one and don't look back.

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Offlinedisturbed
Poutine andSodomy
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Registered: 10/15/02
Posts: 1,877
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Last seen: 14 years, 5 months
Re: Anyone have experience with fractional sterilization? [Re: Aeolus1369]
    #2456185 - 03/21/04 01:10 AM (20 years, 2 days ago)

defintiely can't do grain with fractional sterilization... Many have had success with fractional sterilization of BRF though, give it a shot.


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11/25/07 first time entrant ban lottery champion

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