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Amazon Shop for: Autoclave, Pressure Cooker, Scales

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OfflineSilven
I trip,therefore weare.

Registered: 08/30/04
Posts: 2,071
Loc: El Mexicano
Last seen: 10 years, 10 months
Fractional Sterilization? Success?
    #3174238 - 09/24/04 11:22 AM (12 years, 5 months ago)

How well does Fractional Sterilization work?  I just stumbled across this tek.

Quote:


1) Steam heating to 100 ?C for 30 min
Vegetative cells are destroyed but endospores survive

2) Incubate at 30?C-37?C overnight
Most bacterial endospores germinate

3) Second heat treatment, 100 ?C, 30 min
Germinated endospores are killed.

4) Second incubation at 30?C-37 ?C overnight
Remaining endospores germinate

5) Third heat treatment, 100 ?C, 60 min
Last remaining germinated endospores are killed





That's directly from the tek located here.

Has anyone tried this?  It's supposedly a way to use grain without using a pressure cooker, allowing the endospores to germinate, and then fries them again.

Any feedback, as always, is greatly appreciated! :smile:

- Silven :mushroom2:


--------------------
Born: 10/31/83, which makes me a Scorpio.

1) Scorpios are the most highly sexed of all the signs of the zodiac.
2) Scorpios are prone to excesses: booze, drugs, sex, bad puns, etc.
They usually exploit the weaknesses of others, who fall victim to
their capacity for total lust & sexual abberation.
3) Scorpios possess great intellectual curiosity & creative talent. They
think they are rebels & are arrogant, proud, conceited, and worth every
penny of it.

What do you bring to the table?


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OfflineAnnoA
Experimenter
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Registered: 06/18/99
Posts: 24,122
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Last seen: 1 month, 15 days
Re: Fractional Sterilization? Success? [Re: Silven]
    #3174250 - 09/24/04 11:26 AM (12 years, 5 months ago)

It works if you don't take shortcuts.
But if you are serious about this hobby no way leads around a good, big pressure cooker.


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InvisiblePrisoner#1M
Even Dumber ThanAdvertized!
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Registered: 01/22/03
Posts: 193,359
Loc: Pvt. Pubfag NutSuck
Trusted Cultivator
Re: Fractional Sterilization? Success? [Re: Silven]
    #3174379 - 09/24/04 12:03 PM (12 years, 5 months ago)

I couldnt agree more with anno...
the money you spend on a PC is the
best investment you will ever make


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Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: Fractional Sterilization? Success? [Re: Silven]
    #3174402 - 09/24/04 12:12 PM (12 years, 5 months ago)

PC is way to go , but if you are really truly into thurough "sterilization " , there is nothing better than an Autoclav !

A lot less time and much more effective , less time required also for the same effects .

#1 Put in AC 250*F for 1 hour (15psi) and wah la all foriengers are dead in one shot , same goes for PC , but AC is much more accurate . PC is much more cost effectient for the average grower ... yadda yadda .


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


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OfflineSilven
I trip,therefore weare.

Registered: 08/30/04
Posts: 2,071
Loc: El Mexicano
Last seen: 10 years, 10 months
Re: Fractional Sterilization? Success? [Re: Anno]
    #3175234 - 09/24/04 03:46 PM (12 years, 5 months ago)

I agree with you Anno.  Being new to mushroom cultivation though I'm just reading as much as I can, trying to weed out information that will be useless to me, etc.. 

I appreciate all the information you have provided to me Anno (including my grow log), thanks!



I don't think I'll ever be cultivating on a high enough scale to move to an Autoclave though, but I will check into pricing as I intend to get to the best possible sterility for my buck, it just seems a PC is the way to go.

I plan on cultivating only for personal use and use with a few trusted friends.

:smile:  Silven :mushroom2:


--------------------
Born: 10/31/83, which makes me a Scorpio.

1) Scorpios are the most highly sexed of all the signs of the zodiac.
2) Scorpios are prone to excesses: booze, drugs, sex, bad puns, etc.
They usually exploit the weaknesses of others, who fall victim to
their capacity for total lust & sexual abberation.
3) Scorpios possess great intellectual curiosity & creative talent. They
think they are rebels & are arrogant, proud, conceited, and worth every
penny of it.

What do you bring to the table?


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletripndicular
My Minds Eye IsRhizomorphic

Registered: 08/25/02
Posts: 2,791
Loc: Bowels of HELL
Re: Fractional Sterilization? Success? [Re: Silven]
    #3175958 - 09/24/04 06:42 PM (12 years, 5 months ago)

ANNO is an excellent choice of mentors ! :thumbup: :cool: :sun:

Small AC can be bought , but they are very pricy .
Do not skimp when buying PC , get one that will be slightly bigger than you think you need . 23QT is awesome to have and can be bought for about 100$ , also get one that has a psi gauge on it .
Good Luck
"May The Mushroom Goddess Bless All Your Endeavors !"
Trip n D


--------------------
Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


Post Extras: Print Post  Remind Me! Notify Moderator
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Amazon Shop for: Autoclave, Pressure Cooker, Scales

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