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Offlineyogenfruzen
Gogs
Registered: 12/03/02
Posts: 51
Loc: FunkyTown
Last seen: 21 years, 6 months
Fractional sterilization
    #1120676 - 12/07/02 09:08 PM (21 years, 7 months ago)

Will fractional sterilization work to sterilize grain? has anyone had success with this method?


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If you drive a drink and a cop pulls you over and says "Your drunk" it's ok because you have no choice, your driving a drink!

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Invisibledog
straw dog

Registered: 06/20/02
Posts: 2,790
Loc: Route 66
Re: Fractional sterilization [Re: yogenfruzen]
    #1120839 - 12/07/02 10:26 PM (21 years, 7 months ago)

I tried several times to sterilize rye berries and then birdseed with this method. Every attempt ended in contamination.


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Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.

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Invisibledaussaulit
Forgetful

Registered: 08/06/02
Posts: 2,894
Loc: Earth
Re: Fractional sterilization [Re: yogenfruzen]
    #1121354 - 12/08/02 01:45 AM (21 years, 7 months ago)

double post, already posted in the advanced cultivation forum.

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OfflineAnnoA
Experimenter
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Re: Fractional sterilization [Re: dog]
    #1121363 - 12/08/02 01:47 AM (21 years, 7 months ago)

Could you describe your precedure exactly?

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Offlineshirley knott
not my real name
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Registered: 11/11/02
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Loc: London
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Trusted Cultivator
Re: Fractional sterilization [Re: yogenfruzen]
    #1121581 - 12/08/02 04:08 AM (21 years, 7 months ago)

and could you explain your signature exactly, too?

It's not in English as I speak it.


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buh

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Offlineyogenfruzen
Gogs
Registered: 12/03/02
Posts: 51
Loc: FunkyTown
Last seen: 21 years, 6 months
Re: Fractional sterilization [Re: yogenfruzen]
    #1122424 - 12/08/02 04:06 PM (21 years, 7 months ago)

well I havn't tried it but I would:

boil 30 min
incubate for 24 hours at 30C
boil 30 min
incubate 24 hours at 30C
boil 60 min

And the signature is just a pearl of wisdom I had whe I was high once lol


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If you drive a drink and a cop pulls you over and says "Your drunk" it's ok because you have no choice, your driving a drink!

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OfflineSkikid16
fungus fan

Registered: 06/27/02
Posts: 5,666
Loc: In the middle of the nort...
Last seen: 19 years, 3 months
Re: Fractional sterilization [Re: yogenfruzen]
    #1122430 - 12/08/02 04:11 PM (21 years, 7 months ago)

Quote:

And the signature is just a pearl of wisdom I had whe I was high once lol 


  I see no wisdom, I've tried making sense of that damn sig for the last week, and I still can't get it, it bothers the hell out of me.  :wink:  Sorry to get off topic, I just had to vent my frustration. 


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Re-Defeat Bush in '04

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Offlineyogenfruzen
Gogs
Registered: 12/03/02
Posts: 51
Loc: FunkyTown
Last seen: 21 years, 6 months
Re: Fractional sterilization [Re: Skikid16]
    #1122434 - 12/08/02 04:16 PM (21 years, 7 months ago)

relax, it's something stupid I said while I was high. I laughed my ass off the next day when I remembered that I convinced a couple people that that was true.


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If you drive a drink and a cop pulls you over and says "Your drunk" it's ok because you have no choice, your driving a drink!

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OfflineSkikid16
fungus fan

Registered: 06/27/02
Posts: 5,666
Loc: In the middle of the nort...
Last seen: 19 years, 3 months
Re: Fractional sterilization [Re: yogenfruzen]
    #1122438 - 12/08/02 04:19 PM (21 years, 7 months ago)

I was joking, sorry if it didn't sound like it.  I was just playing.  :tongue:  But I still can't understand it.  I'm serious, I've read it when I was sober, stoned, drunk, a combo of the last two, and I can't get it, damn damn damn.  Oh well.  ok, back on to fractal sterilization. :wink:


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Re-Defeat Bush in '04

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Offlineyogenfruzen
Gogs
Registered: 12/03/02
Posts: 51
Loc: FunkyTown
Last seen: 21 years, 6 months
Re: Fractional sterilization [Re: Skikid16]
    #1122451 - 12/08/02 04:23 PM (21 years, 7 months ago)

It's not supposed to make sence. You can't drive a drink, a drink cannot be driven. I just find it funny when I read it. If you ever actually understand it, you must have like 20g of shrooms in you.


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If you drive a drink and a cop pulls you over and says "Your drunk" it's ok because you have no choice, your driving a drink!

Edited by yogenfruzen (12/08/02 04:25 PM)

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InvisibleSixCee
keep rolling
Male

Registered: 06/12/02
Posts: 3,720
Loc: US, Chicago
Re: Fractional sterilization [Re: yogenfruzen]
    #1122550 - 12/08/02 05:29 PM (21 years, 7 months ago)

I say stuff like that all the time when I'm toasted.


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~~~~~~~~~~~~~~~~~~~~~~~~
-->The above statements may or not be true.
->Quote of the Moment :
"Yea. All bitches are whores who love sex." -Cubie
----> PMs checked daily.

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Offlineyogenfruzen
Gogs
Registered: 12/03/02
Posts: 51
Loc: FunkyTown
Last seen: 21 years, 6 months
Re: Fractional sterilization [Re: SixCee]
    #1122577 - 12/08/02 05:41 PM (21 years, 7 months ago)

ya I find it hilarious when you reminiss about the shit you said or did, the day after with the friends you were baked with.


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If you drive a drink and a cop pulls you over and says "Your drunk" it's ok because you have no choice, your driving a drink!

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Invisibledog
straw dog

Registered: 06/20/02
Posts: 2,790
Loc: Route 66
Re: Fractional sterilization [Re: Anno]
    #1122907 - 12/08/02 08:18 PM (21 years, 7 months ago)

Quote:

Could you describe your precedure exactly?




For the rye berries I first simmered the rye at a low boil for 40 minutes. I then dumped the rye into a strainer and let it drain. After it was well drained I loaded it into a quart jar 2/3 full, put a filter disk on it, covered the disk with a lid that had two 3/8" holes drilled into it and then secured it all with the ring. I covered the filtered holes with duct tape, boiled the jar for 1 hour and then let it sit in the pot for 24 hours at about 85F. I repeated this three times. On day four I removed the jar from the pot, removed the tape from the filtered holes and then inoculated through one of them. The tape was then replaced over the inoculation hole, leaving one filtered hole for air exchange. After incubating for three days I checked the jar and was brought to my knees by the stench.  :crazy:

The birdseed jar was prepared the same way, except that I soaked the birdseed in water overnight rather than simmering it. The resulting smell was just as lovely.

I'm wondering now if I might have had better luck using smaller jars so that the heat could penetrate more easily.  :confused:   


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Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.

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InvisibleSixCee
keep rolling
Male

Registered: 06/12/02
Posts: 3,720
Loc: US, Chicago
Re: Fractional sterilization [Re: dog]
    #1122934 - 12/08/02 08:24 PM (21 years, 7 months ago)

When using grains as a substrate a pressure cooker is a must.


--------------------
~~~~~~~~~~~~~~~~~~~~~~~~
-->The above statements may or not be true.
->Quote of the Moment :
"Yea. All bitches are whores who love sex." -Cubie
----> PMs checked daily.

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Offlineyogenfruzen
Gogs
Registered: 12/03/02
Posts: 51
Loc: FunkyTown
Last seen: 21 years, 6 months
Re: Fractional sterilization [Re: dog]
    #1123166 - 12/08/02 09:22 PM (21 years, 7 months ago)

Dog your problem might have been your incubation temp. You had it at 85 when it should be between 86F - 99F. I'm going to try this using a pint jar and a tall 1/2 pint jar. I'll post my results when I do this which willl be in the next 3-4 weeks.


--------------------
If you drive a drink and a cop pulls you over and says "Your drunk" it's ok because you have no choice, your driving a drink!

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Invisibledog
straw dog

Registered: 06/20/02
Posts: 2,790
Loc: Route 66
Re: Fractional sterilization [Re: yogenfruzen]
    #1123312 - 12/08/02 10:12 PM (21 years, 7 months ago)

The 85F was to encourage germination any endospores residing in the grain so that they could be killed in the next round of boiling. 85F is also ideal for incubating cubensis. Check out this chart from the FAQ:



If you try to incubate at much more than 88F you are going to be waiting for a fuck of a long time.


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Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.

Edited by dog (12/09/02 08:29 AM)

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Offlineyogenfruzen
Gogs
Registered: 12/03/02
Posts: 51
Loc: FunkyTown
Last seen: 21 years, 6 months
Re: Fractional sterilization [Re: dog]
    #1125318 - 12/09/02 02:59 PM (21 years, 7 months ago)

when I said your incubation temp should have been between 86 - 99 I meant while incubating the endospores during fractional sterilization not after inoculation, I thought that was pretty obvious. your incubation temp (for the endospores!) of 85F was probably too low and didn't cause all of the endospores to germinate, therefore letting them survive the boilings


--------------------
If you drive a drink and a cop pulls you over and says "Your drunk" it's ok because you have no choice, your driving a drink!

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OfflinePooGrower
Mr. Hanky

Registered: 11/01/02
Posts: 542
Loc: East Side
Last seen: 18 years, 4 months
Re: Fractional sterilization [Re: yogenfruzen]
    #1125345 - 12/09/02 03:04 PM (21 years, 7 months ago)

Read lots of teks on sterilization And do exactly as they say and maybe then some if not whats the point in trying alot will get contamed its the number step and most important in my words


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"I WONT STOP TILL I HAVE A LIFE TIME SUPPLY" "dont be offended by anything i say, its in a joking manner"

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OfflineAnnoA
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Re: Fractional sterilization [Re: dog]
    #1125346 - 12/09/02 03:04 PM (21 years, 7 months ago)

I did the same procedure you described, and the rye jars turned out perfectly, contaminant free and colonized completely in less than 2 weeks.


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Offlineyogenfruzen
Gogs
Registered: 12/03/02
Posts: 51
Loc: FunkyTown
Last seen: 21 years, 6 months
Re: Fractional sterilization [Re: Anno]
    #1126069 - 12/09/02 06:36 PM (21 years, 7 months ago)

hey poo grower, Enlish isn't your first language is it.


--------------------
If you drive a drink and a cop pulls you over and says "Your drunk" it's ok because you have no choice, your driving a drink!

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