Home | Community | Message Board

Mushrooms.com
Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Kratom Powder for Sale   Bridgetown Botanicals CBD Edibles   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   Kraken Kratom Red Vein Kratom   North Spore Bulk Substrate, Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Original Sensible Seeds Autoflowering Cannabis Seeds, Bulk Cannabis Seeds, Feminized Cannabis Seeds, High THC Strains, USA West Coast Strains

Jump to first unread post Pages: 1
OfflineReeFuSEtoLose
Stranger

Registered: 01/15/05
Posts: 46
Last seen: 15 years, 7 months
How do i achieve proper water content, while fractionally sterillizeing grain?
    #3689832 - 01/27/05 07:44 PM (16 years, 8 months ago)

Im trying fractional sterillization for the first time, becuse i no longer have access to a p.c., im useing bird feed as substrate and i was wondering, should i steep the birdseed before i start the fractionall sterillization?P and T for your responses


--------------------
Peaze
---------------------------------------
even a broken clock is still right twice a day.


Extras: Filter Print Post Remind Me! Notify Moderator Top
Offlinekiss
numbnuts
 User Gallery

Registered: 12/14/04
Posts: 486
Loc: under your bed
Last seen: 6 years, 10 months
Re: How do i achieve proper water content, while fractionally sterillizeing grain? [Re: ReeFuSEtoLose]
    #3689993 - 01/27/05 08:13 PM (16 years, 8 months ago)

I don't know what fractional steri. is...maybe boiling?
If that's the case then I think it would be next to impossible to do that and be able to call the substrate 100% sterilized.
As I understand it, you'd be lucky to kill half of the endospores, or even a fraction (no pun intended) with that kind of heat.
If you don't have a P.cooker at your disposal, then you'd be better off doing the PFtek which uses brown rice flour.
Maybe others have tried this method with some degree of success, but certainly not constant.
edited for miss spelling :smirk:


--------------------
see dick drink
see dick drive
see dick die
don't be a dick!


Edited by kiss (01/27/05 08:15 PM)


Extras: Filter Print Post Remind Me! Notify Moderator Top
InvisibleNOS4A2
This is the way

Registered: 07/22/04
Posts: 572
Loc: -tite
Re: How do i achieve proper water content, while fractionally sterillizeing grain? [Re: kiss]
    #3690020 - 01/27/05 08:19 PM (16 years, 8 months ago)

Hey I tried it last time and it really sucks!


--------------------


Extras: Filter Print Post Remind Me! Notify Moderator Top
InvisibleNOS4A2
This is the way

Registered: 07/22/04
Posts: 572
Loc: -tite
Re: How do i achieve proper water content, while fractionally sterillizeing grain? [Re: NOS4A2]
    #3690027 - 01/27/05 08:21 PM (16 years, 8 months ago)

I think it would take alot of trial and error on your part and if you switched grain type you'd have to start all over. I F_S'ed some WBS and it turned out very mushy. I was trying to sterilize with out a PC cause the one I had sucked!


--------------------


Extras: Filter Print Post Remind Me! Notify Moderator Top
Invisiblemetasin
Stranger
 User Gallery

Registered: 10/12/04
Posts: 972
Re: How do i achieve proper water content, while fractionally sterillizeing grain? [Re: kiss]
    #3690030 - 01/27/05 08:22 PM (16 years, 8 months ago)

Fractional sterilization involves boiling the WBS three times, one time a day for three days. Its supposed to work sometimes.

Water content: The overnight soaking of the grain then the 20-30 minute simmer takes care of the correct water content for you.


Extras: Filter Print Post Remind Me! Notify Moderator Top
InvisibleNOS4A2
This is the way

Registered: 07/22/04
Posts: 572
Loc: -tite
Re: How do i achieve proper water content, while fractionally sterillizeing grain? [Re: metasin]
    #3690046 - 01/27/05 08:25 PM (16 years, 8 months ago)

The problem comes in the three boils(water content) unless you watched closely and boiled at as low a temp as possible. I don't know JMO, it must work or people wouldn't make a tech 4 it, but with grains there is too much to mess up IMO-


--------------------


Extras: Filter Print Post Remind Me! Notify Moderator Top
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,145
Loc: my room
Last seen: 9 hours, 48 minutes
Re: How do i achieve proper water content, while fractionally sterillizeing grain? [Re: ReeFuSEtoLose]
    #3690057 - 01/27/05 08:28 PM (16 years, 8 months ago)

You can do it either by soaking/simmering the grain first, or by adding the measured amounts of water and grain to the jars directly. Should you notice that the grain is too dry after the first boiling and shaking, you can add some more water.


Extras: Filter Print Post Remind Me! Notify Moderator Top
OfflineReeFuSEtoLose
Stranger

Registered: 01/15/05
Posts: 46
Last seen: 15 years, 7 months
Re: How do i achieve proper water content, while fractionally sterillizeing grain? [Re: Anno]
    #3690077 - 01/27/05 08:36 PM (16 years, 8 months ago)

thanks anno, thats more of the kind of answer i was looking for. Do you think that fractional sterilization actually works? Because theoretically its all there. you know the whole germintation of threatening endospores, and then killing them off with later half hour boils.


--------------------
Peaze
---------------------------------------
even a broken clock is still right twice a day.


Extras: Filter Print Post Remind Me! Notify Moderator Top
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,145
Loc: my room
Last seen: 9 hours, 48 minutes
Re: How do i achieve proper water content, while fractionally sterillizeing grain? [Re: ReeFuSEtoLose]
    #3690156 - 01/27/05 09:01 PM (16 years, 8 months ago)

> Do you think that fractional sterilization actually works?

I tested it out a couple of times with rye, it worked for me.
But it takes long, so if possible, try to get a pressure cooker.


Extras: Filter Print Post Remind Me! Notify Moderator Top
OfflineReeFuSEtoLose
Stranger

Registered: 01/15/05
Posts: 46
Last seen: 15 years, 7 months
Re: How do i achieve proper water content, while fractionally sterillizeing grain? [Re: Anno]
    #3690190 - 01/27/05 09:11 PM (16 years, 8 months ago)

thanks thats all i wanted to hear, p.c.'s run about 150 bucks at the only store i can find them in, so im going to attempt this technique with finch seed and post the results


--------------------
Peaze
---------------------------------------
even a broken clock is still right twice a day.


Extras: Filter Print Post Remind Me! Notify Moderator Top
Invisiblepinkfloydms
!!!!!
Male

Registered: 05/27/04
Posts: 4,470
Loc: City of Dreams
Re: How do i achieve proper water content, while fractionally sterillizeing grain? [Re: Anno]
    #3691260 - 01/27/05 11:52 PM (16 years, 8 months ago)

Anno would you recommend letting the jars used in fractional sterilization sit for about a week or so before inocc. to see if any contams grow? Or would that be worse?


--------------------
Muppet Said:

so yeah:
- 'sex' five times
- once with a man
- once with a cadaver
- and thrice with actual women(all of which were prostitutes)
Best story ever!

www.panicstream.com :thumbup:


Extras: Filter Print Post Remind Me! Notify Moderator Top
Invisiblepinkfloydms
!!!!!
Male

Registered: 05/27/04
Posts: 4,470
Loc: City of Dreams
Re: How do i achieve proper water content, while fractionally sterillizeing grain? [Re: pinkfloydms]
    #3691544 - 01/28/05 12:38 AM (16 years, 8 months ago)

Can anyone answer this question for me please?


--------------------
Muppet Said:

so yeah:
- 'sex' five times
- once with a man
- once with a cadaver
- and thrice with actual women(all of which were prostitutes)
Best story ever!

www.panicstream.com :thumbup:


Extras: Filter Print Post Remind Me! Notify Moderator Top
InvisiblePrisoner#1
Even Dumber ThanAdvertized!
 User Gallery

Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
Trusted Cultivator
Re: How do i achieve proper water content, while fractionally sterillizeing grain? [Re: pinkfloydms]
    #3691868 - 01/28/05 02:24 AM (16 years, 8 months ago)

give them 4-5 days of rest to check for contams, if you add a little gypsum it
will help to stabilize the water content of the grain and prevent bacterial contams


Extras: Filter Print Post Remind Me! Notify Moderator Top
Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 8 years, 9 months
Trusted Cultivator
Re: How do i achieve proper water content, while fractionally sterillizeing grain? [Re: NOS4A2]
    #3692988 - 01/28/05 12:26 PM (16 years, 8 months ago)

Quote:

NOS4A2 said:
The problem comes in the three boils(water content) unless you watched closely and boiled at as low a temp as possible. I don't know JMO, it must work or people wouldn't make a tech 4 it, but with grains there is too much to mess up IMO-




It doesnt work like that. The 3 boils dont go in place of simmering..your boiling the jars filled with substrate 3 times..in place of pressure cooking. The water content should not change from before your first boil to after your third. First get the water content where it needs to be by soaking and/or simmering...then load..then sterilize.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Extras: Filter Print Post Remind Me! Notify Moderator Top
Offlineblackout
 User Gallery

Registered: 07/17/00
Posts: 5,136
Last seen: 2 days, 9 hours
Trusted Cultivator
Re: How do i achieve proper water content, while fractionally sterillizeing grain? [Re: scatmanrav]
    #4016667 - 04/05/05 12:27 PM (16 years, 6 months ago)

Has anybody had success with this yet besides Anno? If your lid is loose water may be lost on each boil. My idea was to fill bags or large jars with grain and microwave them. 1ml of water weighs approx 1g so you can weigh your jars and grain and add excess water. Then boil it down over a few days until it weighs exactly what it should with your correct water content. This way you can handle large bags without the need for a large PC. I had success in sterilising rye grass seed, rice and wheat, got some bags started but lost them to contamination. This could have been contaminated spores since I made the syringes myself and was not overly careful.


Extras: Filter Print Post Remind Me! Notify Moderator Top
Offlinepsilocyben
bad guy

Registered: 12/03/04
Posts: 1,026
Last seen: 14 years, 9 months
Re: How do i achieve proper water content, while fractionally sterillizeing grain? [Re: ReeFuSEtoLose]
    #4016924 - 04/05/05 01:34 PM (16 years, 6 months ago)

Quote:

ReeFuSEtoLose said:
thanks thats all i wanted to hear, p.c.'s run about 150 bucks at the only store i can find them in, so im going to attempt this technique with finch seed and post the results




when, (and i say that with a smirk of confidence) :naughty:
you get a pressure cooker,
do it right and get an
all american

until then, good luck with your results on the fract. sterilization.
:thumbup:


--------------------




Extras: Filter Print Post Remind Me! Notify Moderator Top
Jump to top Pages: 1

Shop: PhytoExtractum Kratom Powder for Sale   Bridgetown Botanicals CBD Edibles   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   Kraken Kratom Red Vein Kratom   North Spore Bulk Substrate, Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Original Sensible Seeds Autoflowering Cannabis Seeds, Bulk Cannabis Seeds, Feminized Cannabis Seeds, High THC Strains, USA West Coast Strains


Similar ThreadsPosterViewsRepliesLast post
* Fractional sterilization yogenfruzen 1,882 19 12/09/02 08:36 PM
by yogenfruzen
* does fractional sterilization work good? c0ma 789 4 01/03/03 08:31 PM
by SomeMycologist
* Fractional sterilization, any one have experience? DrD525 876 3 06/12/02 02:56 PM
by DrD525
* fractional sterilization SomeMycologist 530 2 07/03/03 01:34 AM
by SomeMycologist
* Water content in Rye Grain shroomeater 1,361 6 02/10/03 01:06 AM
by fugu
* Pre sterilized grain? Chemical_Smile 689 5 04/07/03 02:19 AM
by Chemical_Smile
* Preparing grain without a pressure cooker The FUnGal FiEnD 2,080 12 02/22/03 09:02 AM
by The FUnGal FiEnD
* soaking grains meatman 1,948 8 10/02/02 05:10 PM
by meatman

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, FooMan, mushboy, fahtster, LogicaL Chaos, 13shrooms, stonesun, wildernessjunkie, cronicr, Pastywhyte, bodhisatta
1,253 topic views. 20 members, 76 guests and 17 web crawlers are browsing this forum.
[ Print Topic | ]
Search this thread:

Copyright 1997-2021 Mind Media. Some rights reserved.

Generated in 0.033 seconds spending 0.008 seconds on 16 queries.