Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery


This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Sporeworks.EU Spores for European Microscopy   Myyco.com Golden Teacher Liquid Culture For Sale   North Spore Bulk Substrate   MagicBag.co Certified Organic All-In-One Grow Bags   Mushroom-Hut Liquid Cultures   Original Sensible Seeds Autoflowering Cannabis Seeds

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
How do i achieve proper water content, while fractionally sterillizeing grain?
    #3689832 -

Im trying fractional sterillization for the first time, becuse i no longer have access to a p.c., im useing bird feed as substrate and i was wondering, should i steep the birdseed before i start the fractionall sterillization?P and T for your responses


--------------------
Peaze
---------------------------------------
even a broken clock is still right twice a day.

Extras: Filter Print Post Top
Re: How do i achieve proper water content, while fractionally sterillizeing grain? [Re: ReeFuSEtoLose]
    #3689993 -

I don't know what fractional steri. is...maybe boiling?
If that's the case then I think it would be next to impossible to do that and be able to call the substrate 100% sterilized.
As I understand it, you'd be lucky to kill half of the endospores, or even a fraction (no pun intended) with that kind of heat.
If you don't have a P.cooker at your disposal, then you'd be better off doing the PFtek which uses brown rice flour.
Maybe others have tried this method with some degree of success, but certainly not constant.
edited for miss spelling :smirk:


--------------------
see dick drink
see dick drive
see dick die
don't be a dick!

Edited by kiss (01/27/05 08:15 PM)

Extras: Filter Print Post Top
Re: How do i achieve proper water content, while fractionally sterillizeing grain? [Re: kiss]
    #3690020 -

Hey I tried it last time and it really sucks!


--------------------

Extras: Filter Print Post Top
Re: How do i achieve proper water content, while fractionally sterillizeing grain? [Re: NOS4A2]
    #3690027 -

I think it would take alot of trial and error on your part and if you switched grain type you'd have to start all over. I F_S'ed some WBS and it turned out very mushy. I was trying to sterilize with out a PC cause the one I had sucked!


--------------------

Extras: Filter Print Post Top
Re: How do i achieve proper water content, while fractionally sterillizeing grain? [Re: kiss]
    #3690030 -

Fractional sterilization involves boiling the WBS three times, one time a day for three days. Its supposed to work sometimes.

Water content: The overnight soaking of the grain then the 20-30 minute simmer takes care of the correct water content for you.

Extras: Filter Print Post Top
Re: How do i achieve proper water content, while fractionally sterillizeing grain? [Re: metasin]
    #3690046 -

The problem comes in the three boils(water content) unless you watched closely and boiled at as low a temp as possible. I don't know JMO, it must work or people wouldn't make a tech 4 it, but with grains there is too much to mess up IMO-


--------------------

Extras: Filter Print Post Top
Re: How do i achieve proper water content, while fractionally sterillizeing grain? [Re: ReeFuSEtoLose]
    #3690057 -

You can do it either by soaking/simmering the grain first, or by adding the measured amounts of water and grain to the jars directly. Should you notice that the grain is too dry after the first boiling and shaking, you can add some more water.


--------------------
Grow Mushrooms from Spores - PF-Tek for Simple Minds

Extras: Filter Print Post Top
Re: How do i achieve proper water content, while fractionally sterillizeing grain? [Re: Anno]
    #3690077 -

thanks anno, thats more of the kind of answer i was looking for. Do you think that fractional sterilization actually works? Because theoretically its all there. you know the whole germintation of threatening endospores, and then killing them off with later half hour boils.


--------------------
Peaze
---------------------------------------
even a broken clock is still right twice a day.

Extras: Filter Print Post Top
Re: How do i achieve proper water content, while fractionally sterillizeing grain? [Re: ReeFuSEtoLose]
    #3690156 -

> Do you think that fractional sterilization actually works?

I tested it out a couple of times with rye, it worked for me.
But it takes long, so if possible, try to get a pressure cooker.


--------------------
Grow Mushrooms from Spores - PF-Tek for Simple Minds

Extras: Filter Print Post Top
Re: How do i achieve proper water content, while fractionally sterillizeing grain? [Re: Anno]
    #3690190 -

thanks thats all i wanted to hear, p.c.'s run about 150 bucks at the only store i can find them in, so im going to attempt this technique with finch seed and post the results


--------------------
Peaze
---------------------------------------
even a broken clock is still right twice a day.

Extras: Filter Print Post Top
Re: How do i achieve proper water content, while fractionally sterillizeing grain? [Re: Anno]
    #3691260 -

Anno would you recommend letting the jars used in fractional sterilization sit for about a week or so before inocc. to see if any contams grow? Or would that be worse?


--------------------
Muppet Said:

so yeah:
- 'sex' five times
- once with a man
- once with a cadaver
- and thrice with actual women(all of which were prostitutes)
Best story ever!

www.panicstream.com :thumbup:

Extras: Filter Print Post Top
Re: How do i achieve proper water content, while fractionally sterillizeing grain? [Re: pinkfloydms]
    #3691544 -

Can anyone answer this question for me please?


--------------------
Muppet Said:

so yeah:
- 'sex' five times
- once with a man
- once with a cadaver
- and thrice with actual women(all of which were prostitutes)
Best story ever!

www.panicstream.com :thumbup:

Extras: Filter Print Post Top
Re: How do i achieve proper water content, while fractionally sterillizeing grain? [Re: pinkfloydms]
    #3691868 -

give them 4-5 days of rest to check for contams, if you add a little gypsum it
will help to stabilize the water content of the grain and prevent bacterial contams


--------------------
California Has Legalized Child Prostitution

Extras: Filter Print Post Top
Re: How do i achieve proper water content, while fractionally sterillizeing grain? [Re: NOS4A2]
    #3692988 -

NOS4A2 said:
The problem comes in the three boils(water content) unless you watched closely and boiled at as low a temp as possible. I don't know JMO, it must work or people wouldn't make a tech 4 it, but with grains there is too much to mess up IMO-


It doesnt work like that. The 3 boils dont go in place of simmering..your boiling the jars filled with substrate 3 times..in place of pressure cooking. The water content should not change from before your first boil to after your third. First get the water content where it needs to be by soaking and/or simmering...then load..then sterilize.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay

Extras: Filter Print Post Top
Re: How do i achieve proper water content, while fractionally sterillizeing grain? [Re: scatmanrav]
    #4016667 -

Has anybody had success with this yet besides Anno? If your lid is loose water may be lost on each boil. My idea was to fill bags or large jars with grain and microwave them. 1ml of water weighs approx 1g so you can weigh your jars and grain and add excess water. Then boil it down over a few days until it weighs exactly what it should with your correct water content. This way you can handle large bags without the need for a large PC. I had success in sterilising rye grass seed, rice and wheat, got some bags started but lost them to contamination. This could have been contaminated spores since I made the syringes myself and was not overly careful.

Extras: Filter Print Post Top
Re: How do i achieve proper water content, while fractionally sterillizeing grain? [Re: ReeFuSEtoLose]
    #4016924 -

ReeFuSEtoLose said:
thanks thats all i wanted to hear, p.c.'s run about 150 bucks at the only store i can find them in, so im going to attempt this technique with finch seed and post the results


when, (and i say that with a smirk of confidence) :naughty:
you get a pressure cooker,
do it right and get an
all american

until then, good luck with your results on the fract. sterilization.
:thumbup:


--------------------



Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Sporeworks.EU Spores for European Microscopy   Myyco.com Golden Teacher Liquid Culture For Sale   North Spore Bulk Substrate   MagicBag.co Certified Organic All-In-One Grow Bags   Mushroom-Hut Liquid Cultures   Original Sensible Seeds Autoflowering Cannabis Seeds

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* Fractional sterilization yogenfruzen 2,348 19 12/09/02 08:36 PM
by yogenfruzen
* does fractional sterilization work good? c0ma 914 4 01/03/03 08:31 PM
by SomeMycologist
* Fractional sterilization, any one have experience? DrD525 996 3 06/12/02 02:56 PM
by DrD525
* fractional sterilization SomeMycologist 616 2 07/03/03 01:34 AM
by SomeMycologist
* Water content in Rye Grain shroomeater 1,827 6 02/10/03 01:06 AM
by fugu
* Pre sterilized grain? Chemical_Smile 820 5 04/07/03 02:19 AM
by Chemical_Smile
* Preparing grain without a pressure cooker The FUnGal FiEnD 2,641 12 02/22/03 09:02 AM
by The FUnGal FiEnD
* soaking grains meatman 2,457 8 10/02/02 05:10 PM
by meatman

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,434 topic views. 12 members, 165 guests and 158 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.03 seconds spending 0.008 seconds on 16 queries.