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Offlinecomario2
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frozen mycelium
    #1123085 - 12/08/02 10:57 PM (14 years, 10 months ago)

how about it? will it survive? inadvertently placed in the freezer for 1 week 1 quart grain jar, 100% colonized, psy cyan :confused: 


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comario


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Edited by comario2 (12/08/02 10:59 PM)


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Offlinezeronio
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Re: frozen mycelium [Re: comario2]
    #1123766 - 12/09/02 03:16 AM (14 years, 10 months ago)

I don't know exactly but look at this post

CdC accidentally froze Pholiota nameko mycellium and it survived.


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Invisiblemobyd
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Re: frozen mycelium [Re: zeronio]
    #1123800 - 12/09/02 03:31 AM (14 years, 10 months ago)

I put a psi. cyanewscens in a shock freezer (Temp. -30?C) over night and it survived.........

mobyd


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Invisible@cro
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Re: frozen mycelium [Re: comario2]
    #1123995 - 12/09/02 05:45 AM (14 years, 10 months ago)

If it was partially dehydrated first, possibly, but water tends to form sharp crystals which, when frozen, lyses cells and destroys everything.  But there are certain sugars that can keep water from making these sharp crystals, same way frogs can survive deep freezes. I have no idea what's in the water of mycelium.  The middle may have not frozen all the way, give it a try.  If the middle could survive your best bet IMO would be casing or mix it with another jar and do bulk.
Good Luck :crazy: 


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Offlinecomario2
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Re: frozen mycelium [Re: @cro]
    #1124348 - 12/09/02 11:11 AM (14 years, 10 months ago)

yup, those crystal formations are what's worrying me. i am now doing a slow defrezing, by moving the jar from freezer to fridge, perhaps if the process is slowed down i may be able to save more of it. thanks for the imkput, i'll let you know


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comario


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Invisible@cro
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Re: frozen mycelium [Re: comario2]
    #1125185 - 12/09/02 04:20 PM (14 years, 10 months ago)

Yah, the only reason I mentioned that whole frog thing was that there must be some kinds of mycelium that survive deep freezes. There are plenty of mushrooms that grow in Alaska and Canada and I doubt if they all start from scratch, I mean spores having to recolonize every year. There must be some that are like perrenials - for lack of a better word. Just not sure if cubies would have ever needed this characteristic and more than likely not since they do start from spores almost every time, jumping from one shit to the next.
But it will be interesting to see. Good Luck.


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Invisiblespores
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Re: frozen mycelium [Re: comario2]
    #1125508 - 12/09/02 05:56 PM (14 years, 10 months ago)

I've frozen azure mycelium for a similar length of time in an attempt to kill a bug infestation, it didn't work, but they still fruited eventually.

DH


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Offlinecomario2
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Re: frozen mycelium [Re: spores]
    #1126202 - 12/09/02 09:12 PM (14 years, 10 months ago)

you mean the freezing didn't work as a pesticide but the myc survived anyway? both the freezing and the bugs and it fruited? congrats :laugh: 


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comario


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Invisiblebrowho4d
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Re: frozen mycelium [Re: spores]
    #1127487 - 12/10/02 03:02 AM (14 years, 10 months ago)

I just got here but why are you freezing things.
Is it some super duper cold shocking tek that has been recently discovered.


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THIS PROCEDURE HAS NOT BEEN VALIDATED FOR STERILITY HOWEVER THOUGH IT HAS BEEN SPRAYED WITH DUSTER.


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Invisiblespores
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Re: frozen mycelium [Re: comario2]
    #1128161 - 12/10/02 10:23 AM (14 years, 10 months ago)

Comario: Yes, it fruited, albeit a weak fruiting. But I credit that more to the shitty conditions for azure cultivation in eastern washington (cold temps and extremely dry/low humidity) than freezing it or the bug troubles early on. After being put outside, the fungus gnats seemed to leave it and it grew fine vegetatively. Pics of one of the tiny fruits I got are on Sporeworks' site.

http://www.sporeworks.com/azurdata.htm

Browho4d: If you'd read my post above you would see I froze it in an attempt to kill fungus gnats that invaded the spawn I used.

DH


Edited by DethHed (12/10/02 10:29 AM)


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Offlinecomario2
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Re: frozen mycelium [Re: spores]
    #1128378 - 12/10/02 12:09 PM (14 years, 10 months ago)

hey dethhed, good to know, i have my hopes up again :laugh: btw, i am very familiar with your '99 experiment, you have been one of my guides in my (yet unsuccesful :crazy:) quest for growing azures 


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comario


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InvisibleOllie88
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Re: frozen mycelium [Re: comario2]
    #13824339 - 01/21/11 03:39 AM (6 years, 8 months ago)

Are there any tricks to reviving dried and freezer stored agar mycelium? I made some new agar dishes and scratched some pieces of mycelium into the new dishes, but none of the new dishes show any life.

I kind of assumed that freezing would not hurt dried mycelium...


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OfflineRogerRabbitM
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Re: frozen mycelium [Re: Ollie88]
    #13825109 - 01/21/11 11:45 AM (6 years, 8 months ago)

It depends on species.  Oyster, P cyanescens, and shiitake can all be frozen and recover.  I'm sure there's others as well, but those can for sure, as can reishi and most polypores.
RR


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Invisiblephalcon005

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Re: frozen mycelium [Re: RogerRabbit]
    #13841008 - 01/24/11 10:24 AM (6 years, 8 months ago)

Quote:

RogerRabbit said:
It depends on species.  Oyster, P cyanescens, and shiitake can all be frozen and recover.  I'm sure there's others as well, but those can for sure, as can reishi and most polypores.
RR




I had a Reishi culture shipped to me about a month ago that arrived frozen solid.  I thawed it out in the fridge and plated it the next day.  It took a little while longer to kick off but is robust as hell now.


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OfflineRogerRabbitM
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Re: frozen mycelium [Re: phalcon005]
    #13841449 - 01/24/11 12:43 PM (6 years, 8 months ago)

Reishi can definitely freeze.  Since the OP edited, I see he's talking about P cyanescens, a species which can survive freezing.
RR


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OfflineCubiac
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Re: frozen mycelium [Re: RogerRabbit]
    #14079749 - 03/07/11 08:33 AM (6 years, 7 months ago)

I tryed to start a DEX-LC from some P.mexicana sclerotia, that were frozen.
And one of the two glasses shows growth of mycelium out of the little sclerotiapieces.


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OfflineSpawnRun
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Re: frozen mycelium [Re: Cubiac]
    #14080032 - 03/07/11 10:33 AM (6 years, 7 months ago)

I've had Oysters recover just fine from freezing, as stated.  My cubes did not recover, however.  As stated, it's definitely species-dependent.


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RogerRabbit said:
oh, so narrow minded.  Just because humans have neural pathways, nerve centers, a brain, etc., that can help trigger physical reactions, doesn't mean plants and/or fungi must have the same systems in order to be aware.  The space shuttle has far different and more complex systems than my little two seat airplane, but that doesn't mean they don't both fly.

Several years ago, I 'trained' mycelium to crawl through a maze.  I proved mycelium has a memory because I could transfer a small piece of mycelium from a completed maze to an identical second maze and the mycelium would take the shortest path to the rye grain at the end.
RR


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