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OfflinePhake_ld
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Registered: 10/08/01
Posts: 230
Last seen: 14 years, 4 months
Mycelium Life Span Questions?
    #467517 - 11/23/01 02:12 PM (15 years, 17 days ago)

What is the life span of mycelium if left un attended?

What is the life span of Mycelium when refridgerated?

What is the life span of Mycelium when place in the freezer?


Edited by Phake_ld (11/23/01 02:13 PM)


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Invisiblealtarego
member
Registered: 10/25/01
Posts: 130
Re: Mycelium Life Span Questions? [Re: Phake_ld]
    #467570 - 11/23/01 03:27 PM (15 years, 17 days ago)

Phake -

This is a rather vague and therefore hard-to-answer question.

Except for the frozen one, assuming you mean your regular kitchen freezer, which would certainly kill the mycelium (H2O expands upon freezing, bursting cells.)

As for the others, it all depends upon what form the mycelium is in, ie on what kind of substrate and what condition it is in when it is stored.

Agar cultures can be stored refridgerated, in slant tubes, in theory, for years, but it is safer in practice to use/re-culture them within 6-12 months.

There are other methods of cold storage (H20, agar/mineral oil, paper, search the archives) that may prolong the lifespan of a particular sample, perhaps into years.

Grain cultures would probably store in the fridge safely for a month or two (no guarantee, though, and it would need to be healthy & clean to begin with.)

As for room temp, the mycelium would continue to grow/consume its substrate at these temps, and it would likely weaken with time if it were not friuted on its schedule. Not to mention that more time simply lets competitor organisms catch up.

What are you trying to do exactly?

Good luck
AE



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OfflinePhake_ld
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Registered: 10/08/01
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Re: Mycelium Life Span Questions? [Re: altarego]
    #467586 - 11/23/01 04:09 PM (15 years, 17 days ago)

Let me give you a little more information. I was planning on following the tek below, but it neglects to say how long the Mycelium can be refidgerated.

And in the preperation of the substrate, it doesn't state wheather or not you bring the water to a boil and then add the substrate?

or bring the substrate to a boil with the water? Here is the tek, after reviewing it, you may be able to answer my two questions.

"Liquid Growth Substrate

Mycelium can be cultured in a liquid and then used to easily inoculate substrate and start mycelium growth much faster than the conventional method of spore inoculation to brown rice/finch seed substrate.

Ingredients

Extra Light Powdered Malt Extract
Distilled Water
Aluminum Foil


Boil 25-26 ounces of the distilled water on medium. Mix 1 ounce by weight or roughly 4% of the extra light malt extract powder available at homebrew supply store or on the web at "brewguys.com". Since we are going to sterilize this mixture, there is no need to let it cool down. Pour this medium into any large jar with a metal lid, and make one small hole in the lid with a nail. Cover the top tightly with aluminum foil. Boil the jar in water for 30 minutes placing the jar on a washcloth and not directly on the bottom of the pot. If you own a pressure cooker, set at 15psi for 20 minutes. Let the jar cool overnight.

The next day take your spore syringe, stick the needle through the hole in the lid, and squeeze in the solution. Cover the top of the jar with aluminum foil again, only not too tightly since you'll want to allow gas exchange. Keep the jar somewhere warm and dark, and agitate it every day. You should notice the solution becoming milky, and towards the end of the incubation you'll see fluffy pieces of mycelium floating around in it. At this point you can keep it in the fridge until you're ready to use it.

When you want to start a mushroom culture somewhere, flame-sterilize the needle of a 10cc syringe, let it cool, stick it through the hole in the top of the lid, and pull in as much solution as you need. You can then inject this into your substrate and it will start growing immediately. This medium can be cased in hay, straw, bird seed, or brown rice flour. Any time you run low on mycelium solution, go through the same process with another jar. However, instead of injecting spores, flame -sterilize a 10cc syringe and pull out a fresh fluffy piece of mycellium from the older jar. Simply inject this mycellium directly into the new medium."

I found this tek at kyambiagifts.com/mushroom-madness


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Invisiblealtarego
member
Registered: 10/25/01
Posts: 130
Re: Mycelium Life Span Questions? [Re: Phake_ld]
    #467594 - 11/23/01 04:21 PM (15 years, 17 days ago)

OK -

I would say that you could keep a liquid culture like this for months, provided you were VERY careful when innoculting/extracting mycelium, to avoid introducing contaminants. You might add peroxide after germination to make it last somewhat longer.

Id say as long as it stays clean looking it should be OK.

As for the other question, "substrate" is the growth medium in question, including water. If you mean when do you add the malt extract, it probably doesn't matter, but there would be no harm in adding it first, especially since it will be P.C'ed.

If you are not planning on pressure cooking, ignore my advice, since I doubt the culture would remain clean for long.

Hope that helps,
- AE


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OfflinePhake_ld
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Registered: 10/08/01
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Re: Mycelium Life Span Questions? [Re: altarego]
    #467631 - 11/23/01 05:32 PM (15 years, 17 days ago)

I truly appreciate the guidence you are providing me with... Can you explain the process of adding peroxide to the culture after it has colonized? (Would i dilute the peroxide? do i just pur it in? or would i inject it in with a spore syringe?)


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Invisiblealtarego
member
Registered: 10/25/01
Posts: 130
Re: Mycelium Life Span Questions? [Re: Phake_ld]
    #467674 - 11/23/01 06:41 PM (15 years, 17 days ago)

Ok -

I would recommend reading up about peroxide teks, or buying Rush Wayne's books @ http://members.aol.com/PeroxyMan/index.html

But the general idea is a max of 6ml 3%H202/liter of liquid (this is for agar, but i think these amounts should work fine for liquid culture). Mind you this hasn't been tested (be me, that is), so you are EXPERIMENTING here. Add it straight from a fresh bottle and a sterile syringe would be a good way to get it in.

And you can add it after germination, not just full colonization.
- AE


Edited by altarego (11/23/01 06:42 PM)


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OfflineDinoMyc
Ipsa scientiapotestas est
Registered: 11/14/99
Posts: 1,080
Last seen: 10 years, 5 months
Re: Mycelium Life Span Questions? [Re: Phake_ld]
    #467689 - 11/23/01 07:11 PM (15 years, 17 days ago)

you should read GGMM and TMC, all your questions will be answered.
and freezing is acceptable to nearly all mushrooms, the only I am aware of (although I am sure there are more) which do not tolorate freezing is paddy straw mushrooms. Standard method is deep freeze in liquid nitrogen.


--------------------
If I made affront, I apologize.
If I made affirmation, I apologize.
I merely came to listen, came to say.


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OfflineShdwstr
FSRCanada
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Registered: 02/18/01
Posts: 2,156
Loc: Ontario, Canada
Last seen: 2 years, 11 months
Re: Mycelium Life Span Questions? [Re: DinoMyc]
    #467746 - 11/23/01 08:54 PM (15 years, 17 days ago)

Freezing is not acceptable to ANY fresh Mushroom. Mushrooms are 90% water, if they are frozen, the water will ezpand, rupturing the cell walls. They will look fine while frozen... and become a black mushy goo when thawed. The freezing process will also kill any mycellium.


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OfflineBeppoMarx
old hand
Registered: 09/01/01
Posts: 1,126
Last seen: 11 years, 8 months
Re: Mycelium Life Span Questions? [Re: Shdwstr]
    #468419 - 11/24/01 04:16 PM (15 years, 16 days ago)

i just froze about 12g of fresh shrooms for 3 days and ate them frozen and still had the trip i expected from the small amount. but keeping on track with the honeywater; its fairly simple and my attempts with microwaving have been 100% successful. take regular tap water and put it in one of your jars about halfway or so and boil it in teh mic. i add about a teaspoon of honey without measuring and nuke it again till it just about boils over, wait a few min and boil again (use on high setting). cap with lid that has no holes and cleaned with alcohol and wait for it to cool. it will suction the lid down and be completely sealed and sterile if you cleaned the lid alright. now to innoc just crack the lid or punch a hole if you like (i just open and shoot in then shut) and then let it sit someplace for a week. CO2 does build up but ive found that with a sealed jar you can just crack open the retaining ring once every day or three to let out CO2 pressure that builds up. i still have my first acadian coast cubie honeywater culture that i started sometime in may sometime and is still useable (probably only about 20cc's left in there but its still alive) hope my method helps your progress.


--------------------
Holy shit people; COMMON SENSE! we were all born with it where did it go?
maybe theres a tek out there to explain how to use it!
BUCKETS BRIGADE left hand man!!!


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