Home | Community | Message Board

Kratom Eye
Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Bulk Substrate, Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Kraken Kratom Red Vein Kratom   Bridgetown Botanicals CBD Concentrates, CBD Oils   Original Sensible Seeds Bulk Cannabis Seeds, High THC Strains   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   PhytoExtractum Kratom Powder for Sale, Maeng Da Thai Kratom Leaf Powder

Jump to first unread post Pages: 1 | 2  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlineragadinks
MrBeatle
 User Gallery

Registered: 10/20/03
Posts: 1,297
Last seen: 8 years, 4 months
Storing mycelium in liquid nitrogen ?
    #2696608 - 05/18/04 04:35 PM (18 years, 3 months ago)

In this article at mushworld.com they say that they store mycelium in liquid nitrogen.
Does anybody know more about this kind of long term storage ?


Extras: Filter Print Post Top
Offlinepeachy
Transientpsychonaut

Registered: 05/05/04
Posts: 41
Loc: Preserved deep within the...
Last seen: 18 years, 1 month
Re: Storing mycelium in liquid nitrogen ? [Re: ragadinks]
    #2696681 - 05/18/04 04:43 PM (18 years, 3 months ago)

Sounds interesting, but the site requires registration :frown:

I wonder how cost effective this would be for the small scale (psychotropic) gardener though?

Ideas?


--------------------
"Time bends, space is boundless. It squashes a man's ego. I feel lonely. Tell me, though... does Man, that marvel of the universe, that glorious paradox who sent me to the stars, still make war against his brother? Keep his neighbor's children starving?"


Extras: Filter Print Post Top
Offlineragadinks
MrBeatle
 User Gallery

Registered: 10/20/03
Posts: 1,297
Last seen: 8 years, 4 months
Re: Storing mycelium in liquid nitrogen ? [Re: peachy]
    #2696768 - 05/18/04 04:54 PM (18 years, 3 months ago)

Quote:

Sounds interesting, but the site requires registration



Registration is very easy and does not cost anything.

Quote:

I wonder how cost effective this would be for the small scale (psychotropic) gardener though?
Ideas?




On that site it says:
Quote:

I recommend minimizing transfers by storing in liquid nitrogen, which if you have a non-mechanical tank is not all that expensive or technical.



I wonder what they mean with inexpensive ?


Extras: Filter Print Post Top
Offlinepeachy
Transientpsychonaut

Registered: 05/05/04
Posts: 41
Loc: Preserved deep within the...
Last seen: 18 years, 1 month
Re: Storing mycelium in liquid nitrogen ? [Re: ragadinks]
    #2696870 - 05/18/04 05:07 PM (18 years, 3 months ago)

Yeap, I think I will register and have a gander at that.
Inexpensive is relative, it could mean to your average commercial edible shroom grower; in which case "inexpensive" to them would be synoynmous to "astronomical" for you or I :smile:

I have nasty visions of me (after "accidentally" ingesting something psychedelic) attempting to retrieve some mycelium to innoculate my latest batch- somehow submersing my hand in liquid nitrogen. Imagine the panic! Then tripping and falling over something and my hand  shattering or some shit. LOL! Too much TV eh?


--------------------
"Time bends, space is boundless. It squashes a man's ego. I feel lonely. Tell me, though... does Man, that marvel of the universe, that glorious paradox who sent me to the stars, still make war against his brother? Keep his neighbor's children starving?"


Extras: Filter Print Post Top
Offlinepeachy
Transientpsychonaut

Registered: 05/05/04
Posts: 41
Loc: Preserved deep within the...
Last seen: 18 years, 1 month
Re: Storing mycelium in liquid nitrogen ? [Re: ragadinks]
    #2697097 - 05/18/04 05:37 PM (18 years, 3 months ago)

Hmm, well the storage (if this is what you'd need) looks  affordable
I suppose my next questions would be:
Where can I find someone unscrupulous enough to fill one such container for me? I suppose extraction of nitrogen in some manner is feasible, but cost-effective? And how long would such a method permit me to store my mycelium-brimming testtube babies?

I suspect the answer to the latter would make it more viable for the smallscale grower just to keep a colonized PF cake in the fridge. A similar type of container states it can hold its contents (180 test tube sized vials) at -150C for 230 days ... That isn't that long if you factor in the lengths you'd have to go to achieve all this in addition to the relatively small capacity of such a container.  Dedicating a suitably large fridge to cake storage sounds waay more sensible I'd have to conclude :smile:

Still, the idea is appealing! :laugh: It'd make for really cold beer too.


--------------------
"Time bends, space is boundless. It squashes a man's ego. I feel lonely. Tell me, though... does Man, that marvel of the universe, that glorious paradox who sent me to the stars, still make war against his brother? Keep his neighbor's children starving?"


Extras: Filter Print Post Top
Offlineragadinks
MrBeatle
 User Gallery

Registered: 10/20/03
Posts: 1,297
Last seen: 8 years, 4 months
Re: Storing mycelium in liquid nitrogen ? [Re: peachy]
    #2697219 - 05/18/04 05:50 PM (18 years, 3 months ago)

It's really not that expensive.
But I guess it wont be as easy to store the mycelium in liquid nitrogen as it would be in a common fridge.
Would the mycelium not die when freezing since the cell walls must burst open when the water freezes in them?

Hmm, that would happen with your beer too, won't it? :frown:


Extras: Filter Print Post Top
InvisibleSpeeker

Registered: 02/11/04
Posts: 847
Re: Storing mycelium in liquid nitrogen ? [Re: ragadinks]
    #2697357 - 05/18/04 06:06 PM (18 years, 3 months ago)

You can use glycerin to prevent that happening at least in a normal freezer ..
Resurrecting a Better Method for Long-Term Storage of Mushroom Cultures

but the writer of that article prefers the idea of using distilled
water for storing cultures..


Edited by speeker (05/18/04 06:14 PM)


Extras: Filter Print Post Top
Offlinepeachy
Transientpsychonaut

Registered: 05/05/04
Posts: 41
Loc: Preserved deep within the...
Last seen: 18 years, 1 month
Re: Storing mycelium in liquid nitrogen ? [Re: Speeker]
    #2697558 - 05/18/04 06:34 PM (18 years, 3 months ago)


Speeker:: Thanks for the link I'm going to give that a try. Distillation is simple enough :smile: And a chest freezer I already have.
Anyone else tried this? Might have to conduct some experiments to establish acceptable temperature norms for this sort of thing. It might also be a very viable way of transporting cultures.

ragadinks:: I suppose so, though I suppose you could just open them before putting them into your nitrogen freezer. Also I imagine you'd have to provide kevlar or equivalent gloves to your friends before passing them around; unless they're not that attached to the skin of their hands lol! K, no more puns.


--------------------
"Time bends, space is boundless. It squashes a man's ego. I feel lonely. Tell me, though... does Man, that marvel of the universe, that glorious paradox who sent me to the stars, still make war against his brother? Keep his neighbor's children starving?"


Extras: Filter Print Post Top
OfflineNoG
journeyman

Registered: 04/15/04
Posts: 98
Last seen: 17 years, 6 months
Re: Storing mycelium in liquid nitrogen ? [Re: peachy]
    #2698585 - 05/18/04 10:29 PM (18 years, 3 months ago)

I see this as being dangerous and unnessasary why store mycelium at this temp ? Its not like mushrooms are going to be extinct anytime soon. I just dont see the applications.


Later NoG


Extras: Filter Print Post Top
Offlinepeachy
Transientpsychonaut

Registered: 05/05/04
Posts: 41
Loc: Preserved deep within the...
Last seen: 18 years, 1 month
Re: Storing mycelium in liquid nitrogen ? [Re: NoG]
    #2699404 - 05/19/04 01:54 AM (18 years, 3 months ago)

Unnecessary? Probably.
Dangerous? Certainly (without the correct environment for application of these techniques)

But still interesting, it'd be good for informational purposes to conduct some experiments and find out how much, for how long and at what cost psilocybin shrooms could be stored in such a fashion. If this is the way to statically preserve your favourite cultures for all time, I want to know about it :smile:


--------------------
"Time bends, space is boundless. It squashes a man's ego. I feel lonely. Tell me, though... does Man, that marvel of the universe, that glorious paradox who sent me to the stars, still make war against his brother? Keep his neighbor's children starving?"


Extras: Filter Print Post Top
Offlinesuboriginal
enthusiast

Registered: 05/06/04
Posts: 278
Last seen: 17 years, 10 months
Re: Storing mycelium in liquid nitrogen ? [Re: ragadinks]
    #2701125 - 05/19/04 01:59 PM (18 years, 3 months ago)

Hi raga, check out the little thread posted lately 'freezing mycelia'... not sure if it is possible but I am heartened by your post that suggests that it is... at risk of repeating myself, the best bet would be to freeze them slowly (wrapped in a towel in the freezer, THEN into liquid nitrogen), to minimise formation of damaging water molecule aggregates, but thaw them fast, to maximise the breakdown of any of these that had formed... this is the strategy adopted by scientists freezing human lymphocytes or cancer cells etc... not all cell-type aggregates can be frozen and successfully brought back to life though (neural networks, for example, are pretty much rooted as soon as they're frozen, which is why 'cryogenic preservation' of peeps or even just their brains to be 'brought back later' seems such a scam to me!), and there is a chance it simply won't work with mycelia of some species... I would like some experienced shroom growers on here that have a 'spare cake' that is partly or newly colonised (and sterile) to simply freeze it (SOLID) for 2 or 3 days in a normal freezer, then thaw it out and nurture it, and see if they can bring it back to life... if they can, it suggests that liquid nitrogen storage could work well, at least for that particular species... maybe worth baring in mind though that temperature fluctuations (even below freezing temp) are bad for bio-sample viability, and if they actually thaw out then are re-frozen, more often than not the entire sample is cactus (not bacteria and viruses though)... which may present problems for the home grower trying to maintain liquid nit' type temps constantly, given the occasional malfunctions/ requirements for defrosting of some freezers etc... also, though you may well know this, peeps have reported in these pages that that they've accidentally frozen their spores-syringes solid in these pages, and most survived this process... spores are a lot hardier than mycelia though...
I can see definite advantages of being able to freeze mycelia... it would essentially mean that you could revisit shroom growing YEARS later, say, if your fave or only natural patches get wiped out/ have buildings buit on them/ desecrated in some way... also if you were into multiple strains, you could stash many away and be in no rush or need to 'maintain' them... it's also a lot quicker colonising a cake from myc than from spores, my guess is that this would even hold true if only 10% of the myc remained viable/ survived the freeze...
Again, I'm throwing up the challenge to someone that is experienced in growing (not me!) to freeze one of their cakes right through (just in a normal freezer), then see if they can bring it back to life... that would answer the question once and for all, re the ability of that particular species to withstand freezing...


--------------------
Peace, love and organic brown rice...


Extras: Filter Print Post Top
OfflineNoG
journeyman

Registered: 04/15/04
Posts: 98
Last seen: 17 years, 6 months
Re: Storing mycelium in liquid nitrogen ? [Re: suboriginal]
    #2703571 - 05/20/04 12:26 AM (18 years, 3 months ago)

im guessing the strong would survive ? or the ones more resistant to being frozen. wouldnt this be isolating what your growing a bit like it could turn out to be only the crapy shrooms survived the freeze etc?


Later NoG


Extras: Filter Print Post Top
Offlinepeachy
Transientpsychonaut

Registered: 05/05/04
Posts: 41
Loc: Preserved deep within the...
Last seen: 18 years, 1 month
Re: Storing mycelium in liquid nitrogen ? [Re: NoG]
    #2704119 - 05/20/04 02:30 AM (18 years, 3 months ago)

Well wouldn't hardier mycelia make for hardier shrooms? We could be pleasantly surprised.

I'm going to give this a try for the sake of experimentation, I've a 5litre cake (Ecuadorians) which'll be fully colonized in a few days that I've no real need for; so I'll bung it in the chestfreezer wrapped in a sterilized towel sealed in a ziplock bag and drop the temp as far as it'll go.


--------------------
"Time bends, space is boundless. It squashes a man's ego. I feel lonely. Tell me, though... does Man, that marvel of the universe, that glorious paradox who sent me to the stars, still make war against his brother? Keep his neighbor's children starving?"


Extras: Filter Print Post Top
InvisibleSpeeker

Registered: 02/11/04
Posts: 847
Re: Storing mycelium in liquid nitrogen ? [Re: peachy]
    #2704248 - 05/20/04 03:00 AM (18 years, 3 months ago)

So you're not going to mix it with glycerin first?
My guess is that it's gonna die without it...  :tongue:



Edited by speeker (05/20/04 03:57 AM)


Extras: Filter Print Post Top
Offlineragadinks
MrBeatle
 User Gallery

Registered: 10/20/03
Posts: 1,297
Last seen: 8 years, 4 months
Re: Storing mycelium in liquid nitrogen ? [Re: peachy]
    #2704604 - 05/20/04 07:42 AM (18 years, 3 months ago)

At least it is worth a try.
Outdoor mycelium also freezes sometimes I guess.
But I think if you do it with liquid nitrogen you must add something like glycerin that keeps the cell walls from bursting.
It's a pity that we do not have any professional grower here that could tell us how it is done in the industry.


Extras: Filter Print Post Top
InvisibleSpeeker

Registered: 02/11/04
Posts: 847
Re: Storing mycelium in liquid nitrogen ? [Re: ragadinks]
    #2704633 - 05/20/04 08:07 AM (18 years, 3 months ago)



Edited by speeker (05/20/04 08:13 AM)


Extras: Filter Print Post Top
Offlineragadinks
MrBeatle
 User Gallery

Registered: 10/20/03
Posts: 1,297
Last seen: 8 years, 4 months
Re: Storing mycelium in liquid nitrogen ? [Re: Speeker]
    #2704650 - 05/20/04 08:25 AM (18 years, 3 months ago)

Seems that it would be as simple as adding some glycerin to the culture - mix it up and deep freeze it in liquid nitrogen.
It's a pity that noone has made some experiences with liquid nitrogen in this forum.


Extras: Filter Print Post Top
OfflineNoG
journeyman

Registered: 04/15/04
Posts: 98
Last seen: 17 years, 6 months
Re: Storing mycelium in liquid nitrogen ? [Re: ragadinks]
    #2704762 - 05/20/04 10:03 AM (18 years, 3 months ago)

i dunno if id want to eat mushrooms off a Glycerin soaked cake. from what i understand you dont want to be eating glycerin. and since the stuff absorbs water its not really gonna be good for your 90% water mushies your trying to grow.

maybe soaking your cake in salt water to lower the freezing point of water would be a safer bet for a short freeze in the freezer. I wouldnt freeze the cake for long. and dunk in fresh water to rinse most of the salt off.


Later NoG


Extras: Filter Print Post Top
InvisibleSpeeker

Registered: 02/11/04
Posts: 847
Re: Storing mycelium in liquid nitrogen ? [Re: NoG]
    #2704886 - 05/20/04 11:07 AM (18 years, 3 months ago)

glycerin is used as sugar substitute as sweetner so you probably can eat it safely.. :wink:


Extras: Filter Print Post Top
Offlinesuboriginal
enthusiast

Registered: 05/06/04
Posts: 278
Last seen: 17 years, 10 months
Re: Storing mycelium in liquid nitrogen ? [Re: Speeker]
    #2705848 - 05/20/04 03:25 PM (18 years, 3 months ago)

Sheesh, OK... glycerin would freeze at liquid nitrogen temps... it is used to store things without actually 'freezing them' at below zero, but not THAT far below zero!!! (like in a normal freezer, lets say... still minimises contams/ metabolism by contams ie. GROWTH of contams, but does not fracture the organelles of what you're trying to preserve, cause the solution doesn't actually freeze at 'moderately' below zero temps...
Second, storing your myc in glycerin (should someone on here try this) at below zero will not result in ingestion of glycerin when you eat the shrooms... the glycerin will be long gone 'in the chain; at that point...
Lastly, GOOD FREAKIN ONYA peachy... do the experiment, freeze that sucker, otherwise we're all just guessing... :-)
PS... freeze it slowly, thaw it quickly, then care for it carefully ... I'm on the edge of my seat for the results...

2nd lastly (!), re 'unnatural selection/ crappy shrooms' by freezing/ thawing/ re-nurturing, you could conceivably select for strains that might survive the cold winters in your own back yard, and re-emerge, say, a year after you had broken up a well colonised (frozen/ thawed/ nurtured) cake onto the right spot... just a wild guess...


--------------------
Peace, love and organic brown rice...


Extras: Filter Print Post Top
Offlineragadinks
MrBeatle
 User Gallery

Registered: 10/20/03
Posts: 1,297
Last seen: 8 years, 4 months
Re: Storing mycelium in liquid nitrogen ? [Re: suboriginal]
    #2710195 - 05/21/04 09:08 AM (18 years, 3 months ago)

Somewhere I have read that the mycelium that survived deep freezing was growing much more aggressive and rizomorphic after thawing.
But there were also more deformation of the fruitbodies grown from that mycelium.
Sorry, I cannot remember anymore where I have read that.
If I find the source again I will post a link.


Extras: Filter Print Post Top
Offlinepeachy
Transientpsychonaut

Registered: 05/05/04
Posts: 41
Loc: Preserved deep within the...
Last seen: 18 years, 1 month
Re: Storing mycelium in liquid nitrogen ? [Re: ragadinks]
    #2710416 - 05/21/04 11:12 AM (18 years, 3 months ago)

Well all this talk has really piqued my curiousity :smile:
So besides the frozen cake I mentioned earlier, I've ordered myself some agar and am going to try several of the various methods of storing mycelium mentioned in the various links in this thread.

So to recap, I'm intending to create:

1) Batch of 15 test tubes of mycelium stored in plain distilled water at  room temperature in a cupboard. Every 30days, I will use a sterile pipette to remove a small ammount to colonize some agar to test viability & growth.

2) Batch of 15 test tubes of mycelium stored in plain distilled water and frozen chest freezer. Similarly tests will be conducted for viability. Given the limitation of my current stock of test-tubes the maximum duration is probably 15 x 30days as I suspect the temperature change to pipette samples out will destroy or weaken the contents of the tubes... (Yes thats if the first test even succeeds which I doubt.)

3) Batch of 15 glycerine & mycelium mix test tubes stored and tested as above. These will be the interesting ones to watch I think.

4) 1 Frozen and sealed PK cake in a ziplock bag. I will cut off small pieces of mycelium from within the freezer each month to avoid exposing the cake too many temperature alterations. Im thinking of trying to construct some sort of sealed glove box with a drawer for mycelium removal in my freezer for this so that the ambient temperature around the cake would be relatively stable.
I would then try to re-animate the pieces of mycelium in various fashions. (Any suggestions?) so as to minimize damage.

When I have more cakes available in the coming months I might try storing some smaller entire cakes or sections of cakes in glycerine; but for now supply is my limitation :smile:

For the record all of this will be attempted with Ecuadorian mycelium from a strain I've had particularly good results from to date. For comparison every "test" will be fruited to completion to allow comparison of fruit speed, quantity and quality. The final test shall be ingestion by five willing participants in a legal environment :smile:

I realize results from this wont be very conclusive nor scientific in the proper sense as I don't have enough controls & environmental control (wish I had lab access). Regardless they should be noteworthy if combined with existing information. I'll start a new thread to recap this once results become available.

Now if there's someone out there with access to a liquid nitrogen storage facility of some description willing to conduct similar tests?


--------------------
"Time bends, space is boundless. It squashes a man's ego. I feel lonely. Tell me, though... does Man, that marvel of the universe, that glorious paradox who sent me to the stars, still make war against his brother? Keep his neighbor's children starving?"


Extras: Filter Print Post Top
Offlinepeachy
Transientpsychonaut

Registered: 05/05/04
Posts: 41
Loc: Preserved deep within the...
Last seen: 18 years, 1 month
Re: Storing mycelium in liquid nitrogen ? [Re: peachy]
    #2710494 - 05/21/04 11:48 AM (18 years, 3 months ago)

FYI, here's the cake from which all the mycelium for what I describe above will come. As you can see its a little bit away from full colonization.





Vital stats:

The substrate is whole rice grain mixed in with ground whole rice flour and and 10% ground quinone. The sterile top layer is my vermiculite substitute which is something made out of crushed corn used for reptile cages. The "lid" is tinfoil with with two coffee filters (one inside, one outside) held on by a giant cable twist and some tape for the final filter. The jar was innoculated late night on 10/05. As you can see its quite a sizeable jar :smile:

Little does this poor organism know of what's in store for it :|


--------------------
"Time bends, space is boundless. It squashes a man's ego. I feel lonely. Tell me, though... does Man, that marvel of the universe, that glorious paradox who sent me to the stars, still make war against his brother? Keep his neighbor's children starving?"


Extras: Filter Print Post Top
Offlineragadinks
MrBeatle
 User Gallery

Registered: 10/20/03
Posts: 1,297
Last seen: 8 years, 4 months
Re: Storing mycelium in liquid nitrogen ? [Re: peachy]
    #2712214 - 05/21/04 07:00 PM (18 years, 3 months ago)

Sounds good !
Curious how the results of that experiment will be ...


Extras: Filter Print Post Top
Offlinesuboriginal
enthusiast

Registered: 05/06/04
Posts: 278
Last seen: 17 years, 10 months
Re: Storing mycelium in liquid nitrogen ? [Re: ragadinks]
    #2712912 - 05/21/04 10:45 PM (18 years, 3 months ago)

I'm loving the enthusiasm and can hardly wait for the results...


--------------------
Peace, love and organic brown rice...


Extras: Filter Print Post Top
Offlinesuboriginal
enthusiast

Registered: 05/06/04
Posts: 278
Last seen: 17 years, 10 months
Re: Storing mycelium in liquid nitrogen ? [Re: suboriginal]
    #2718866 - 05/23/04 05:08 PM (18 years, 2 months ago)

I don't think the lack of controls is a big problem re 'the fundamental question', that is can mycelia withstand freezing... sounds like you will be able to answer that unequivicanly once and for all, re your strain. The 'speed of fruiting/ morphology of fruits' etc may well differ from what you're used to (and this is where you'd really need to run 'fresh' controls each time to get an answer), but again I don't think that matters too much... I suspect that a few generations later (with no freezing) the more aggressive etc variants would preveil, and the phenotype re growth/ fruiting rates etc will be restored to resemble that of the original strain you froze in the first place. Just guesses, I'm hanging for the actual results...


--------------------
Peace, love and organic brown rice...


Extras: Filter Print Post Top
Offlinesuboriginal
enthusiast

Registered: 05/06/04
Posts: 278
Last seen: 17 years, 10 months
Re: Storing mycelium in liquid nitrogen ? [Re: ragadinks]
    #2721723 - 05/24/04 08:49 AM (18 years, 2 months ago)

Thought I would add this to this thread, it's with regard to P. cubensis, from a thread called 'P.cubensis mycelia may withstand freezing' or sommat like that, posted by a 'chrisdab':

Ive dunked a cake before and had it frozen for aprox. 24 hours. The mycelium survived and after 3 weeks the second flush appeared.
(posted by "chrisdab" in another thread)


--------------------
Peace, love and organic brown rice...


Extras: Filter Print Post Top
InvisibleSpeeker

Registered: 02/11/04
Posts: 847
Re: Storing mycelium in liquid nitrogen ? [Re: ragadinks]
    #2748719 - 05/31/04 03:01 AM (18 years, 2 months ago)

CRYOPRESERVATION OF FUNGI
WFCC Technical Information Sheets

other storage related sheets..
FREEZE-DRYING OF MICROORGANISM USING A SIMPLE APPARATUS
LIQUID-DRYING OF MICROORGANISM USING A SIMPLE APPARATUS


Extras: Filter Print Post Top
Jump to top Pages: 1 | 2  [ show all ]

Shop: North Spore Bulk Substrate, Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Kraken Kratom Red Vein Kratom   Bridgetown Botanicals CBD Concentrates, CBD Oils   Original Sensible Seeds Bulk Cannabis Seeds, High THC Strains   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   PhytoExtractum Kratom Powder for Sale, Maeng Da Thai Kratom Leaf Powder


Similar ThreadsPosterViewsRepliesLast post
* h TheHateCamel 1,939 6 05/20/04 03:29 AM
by chrisdab
* I am looking for the storing slants in water faq? OoSaToriBluEoO 2,033 4 06/15/01 08:07 PM
by OoSaToriBluEoO
* Nitrogen as a limiting nutrient thisspock 1,588 4 02/19/04 01:00 AM
by Joshua
* Liquid mycelium Visigoth 1,850 10 08/28/02 02:07 PM
by tchyted
* Storing cultures in mineral oil ?
( 1 2 all )
ragadinks 4,205 21 10/02/08 06:11 PM
by OZZ
* Mycelium Life Span Questions? Phake_ld 3,462 8 11/24/01 04:16 PM
by BeppoMarx
* Re: can you save/preserve mycelium for later use Anonymous 4,336 4 07/27/00 10:46 PM
by Anonymous
* Honey mycelium... wikedanjel 2,664 8 07/30/02 11:05 AM
by CaliChronic

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, cronicr, Pastywhyte, bodhisatta
6,135 topic views. 1 members, 6 guests and 1 web crawlers are browsing this forum.
[ Print Topic | ]
Search this thread:

Copyright 1997-2022 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.005 seconds on 13 queries.