frozen substrate......what do u think? - Advanced Mycology

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incredibledibll
Stranger

Registered: 06/27/03
Posts: 15
Last seen: 14 years, 6 months

frozen substrate......what do u think?

#1874535 - 09/02/03 05:03 PM (14 years, 7 months ago)

hello all...
i was wondering if the substrate of the pf tek method can be preserved through freezing in the freezer....do you think the substrate (verm, brf, water) would be usable once defrosted at a later date?
please let me know
thanks...
increbdibll....

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Asmbandits
dimesize

Registered: 04/03/03
Posts: 267
Loc: cow feild
Last seen: 3 years, 1 month

Re: frozen substrate......what do u think?

[Re: incredibledibll]
#1874759 - 09/02/03 06:01 PM (14 years, 7 months ago)

do you mean frezze the sub. before or after it it sterilized?

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Anonymous

Post deleted by Papaver

[Re: Asmbandits]
#1874784 - 09/02/03 06:07 PM (14 years, 7 months ago)

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incredibledibll
Stranger

Registered: 06/27/03
Posts: 15
Last seen: 14 years, 6 months

Re: frozen substrate......what do u think?

[Re: ]
#1876102 - 09/03/03 01:01 AM (14 years, 7 months ago)

no, i mean just freezing the substrate for later use.
as in , the brf and verm mixture in proper ratio, frozen in container, to be sterilized and used at a later time...
please let me know.
thanks
incred.

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deanofmean
mycophagous

Registered: 12/07/02
Posts: 2,017
Loc: PNW

Re: frozen substrate......what do u think?

[Re: incredibledibll]
#1876128 - 09/03/03 01:11 AM (14 years, 7 months ago)

my magic 8 Ball said; doubtful . :shake:

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zeronio
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Registered: 10/16/01
Posts: 2,349
Loc: Slovenia
Last seen: 1 year, 7 months

Re: frozen substrate......what do u think?

[Re: deanofmean]
#1876366 - 09/03/03 02:53 AM (14 years, 7 months ago)

It should work. Freezing does not stop contaminants but it slows them down so if you sterilize the substrate after defrosting it might be OK.

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botanisthype
P$ylocibe$ounDW@vE!

Registered: 07/31/03
Posts: 839
Loc: DECEPTICON MANOR

Re: frozen substrate......what do u think?

[Re: zeronio]
#1879925 - 09/04/03 02:21 AM (14 years, 7 months ago)

yeh sounds logical!

--------------------

Please +REP if i've been of any help... thanks!!!

Dinosaur of the Funny Family, though I have short arms, they fit in my GB... lol

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koraks

Registered: 06/02/03
Posts: 25,433

Re: frozen substrate......what do u think?

[Re: botanisthype]
#1880416 - 09/04/03 07:45 AM (14 years, 7 months ago)

Actually, I think it's a great idea. As our wise man zeronio stated:

Quote:

Freezing does not stop contaminants but it slows them down

Which I believe is very true indeed, so it'd be a nice way to preserve some sterilized substrate in case you're not able to inoculate it immediately after sterilization/cooling down.

But I wonder, deanofmean, why do you think it wouldn't work? As far as I know you're a very experienced member of this community, so I'm really interested in arguments as to why it wouldn't work. Not trying to critisize you here, don't get me wrong, just interested :smile:

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incredibledibll
Stranger

Registered: 06/27/03
Posts: 15
Last seen: 14 years, 6 months

Re: frozen substrate......what do u think?

[Re: koraks]
#1880465 - 09/04/03 08:39 AM (14 years, 7 months ago)

yeah thanks for the input....
however, the bottom line, will it work or not.
b/c my friend doesnt want to innoculate a batch in like 'dead' substrate, if thats whats the freezing light do to it.....
please let me know
inCreD

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incredibledibll
Stranger

Registered: 06/27/03
Posts: 15
Last seen: 14 years, 6 months

Re: frozen substrate......what do u think?

[Re: incredibledibll]
#1880466 - 09/04/03 08:40 AM (14 years, 7 months ago)

light*might

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deanofmean
mycophagous

Registered: 12/07/02
Posts: 2,017
Loc: PNW

Re: frozen substrate......what do u think?

[Re: koraks]
#1881133 - 09/04/03 02:39 PM (14 years, 7 months ago)

didn't say it wouldn't work .
i just don't see any benefit .
don't add water until you are ready to sterilize .
my .02
freezing and thawing, the Ramsie seal will soak up moisture .

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