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 OutThisLife
Registered: 03/27/09
Posts: 1,296
Loc: 127.0.0.1
Last seen: 4 years, 1 month
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 Needles. 
#10203014 - 04/20/09 05:51 PM (14 years, 11 months ago) |
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So my friend brought home extra needles, and she works w/ sterility all the time and gave this suggestion:
To inject a jar w/ 2cc of spores, then throw out that needle and use another one. Repeating between jars for maximum sterility.
She says that contams/junk can easily build up in the needles themselves, and burning doesn't really do anything but burn the junk - which you're putting into your jar.
Would changing out needles between jars be a good idea? Sounds foolproof to me haha.
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Noobey
Noob tube
Registered: 02/15/09
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Would be a huge pain in the ass and you wold go through a shit ton of needles. If you were doing alot of jars you would go insaine. I have re-used my needles alot. Before i use it i soak the needle tip and cap in alcohol, then flame it before ever injection. Worked great so far!
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OutThisLife
Registered: 03/27/09
Posts: 1,296
Loc: 127.0.0.1
Last seen: 4 years, 1 month
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Re: Needles. [Re: Noobey]
#10203052 - 04/20/09 05:59 PM (14 years, 11 months ago) |
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Well the process is actually simple, since they snap on and off the syringes relatively easy. I don't open the case where the needle is until I'm in position to quickly inject.
I don't know if this is even the main source of contams within the jars though for this to really make a difference.
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oh_you_know
lsd <3
Registered: 02/08/09
Posts: 2,282
Loc: Oh, USA
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depends if you have to buy all the needles or if your friend can just get a bunch from work for free
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Noobey
Noob tube
Registered: 02/15/09
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Trust me it would be a pain in the ass. Have you knockedj ars up before. Think about it 4 needles per jar. Thats just dumb.
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Noobey
Noob tube
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Re: Needles. [Re: Noobey]
#10203076 - 04/20/09 06:04 PM (14 years, 11 months ago) |
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I would kill my self if i was knockin up like 25 jars especially in a gove box.
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veda_sticks
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Registered: 07/29/07
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Re: Needles. [Re: Noobey]
#10203089 - 04/20/09 06:07 PM (14 years, 11 months ago) |
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Trust me, when you flame a needle white hot, its sterile wether or not there is still burnt junk in the needle.
Nothing will survive the temp of a white hot needle, nor a red hot needle. And i say white hot because i dont use a regular flame. I use a blotorch that gets the needle white hot.
Having a little bit of burnt junk getting into your jars inst any concern as its sterile, its different in the case of using needles in animals and humans which could be disasterous,.
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OutThisLife
Registered: 03/27/09
Posts: 1,296
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1 needle per jar, doing rye.
I get it though.
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ScavengerType
Registered: 01/24/08
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Loc: The North
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Re: Needles. [Re: Noobey]
#10203118 - 04/20/09 06:16 PM (14 years, 11 months ago) |
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I think your friend is full of shit and I'm betting she's a nurse. When it comes to your human body the standards of what is sterile and what kinds of organisms can contaminate the body vs your cakes is an entirely different field. Does she think your using them for heroin or something? Also you should likely get the kinds of needles you find at an agriculture supply store. Big long thick gauge needles are particularly important with liquid cultures. I can confidently say I've never had a contaminant in my needle.
Now for how your friend is wrong. When you flame sterilize your needles to red hot it expels liquid in the form of steam as well as heating any contaminants. The practical effect is that the heat kills and the germs are expelled by the expanding steam anyway. Some organisms like viruses that can survive the the heat and the steam expulsion could infect your blood but non if any of the contaminants of mushroom substrates is in those list of contaminants.
Your friend may be a good at their medical job but trust me on this all the experts swear by flame sterilization of needles.
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OutThisLife
Registered: 03/27/09
Posts: 1,296
Loc: 127.0.0.1
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^ dentist actually.
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ScavengerType
Registered: 01/24/08
Posts: 5,784
Loc: The North
Last seen: 10 years, 5 months
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same thing though they inject drugs in a medical capacity and that isn't the same as what's going on here.
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"Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
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 MEEZIE
Incognito
Registered: 02/28/09
Posts: 711
Loc: USA 
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I would seem like a wase of time, money, effort considering the flaming you needle between shots is a standard procedure that seems to works for everyone. I think it is just one of those things that would be completely not needed, and just be the extra hassle.
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MEEZIE
Incognito
Registered: 02/28/09
Posts: 711
Loc: USA
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Re: Needles. [Re: MEEZIE]
#10203491 - 04/20/09 07:31 PM (14 years, 11 months ago) |
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Wow i cant spell for shit. Anyways, atleast your head is in the right state of mind as far as being sterile. IT couldnt hurt, but i wouldnt think you need it at all
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OutThisLife
Registered: 03/27/09
Posts: 1,296
Loc: 127.0.0.1
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Re: Needles. [Re: MEEZIE]
#10203707 - 04/20/09 08:08 PM (14 years, 11 months ago) |
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I get all the sterilization equipment for free, along with Birex which apparently can kill HIV so says the packaging :-p
It's whatever, the process is incredibly simple to switch needles the way they have them set up. I get 50 of em' free, why not? :]
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MEEZIE
Incognito
Registered: 02/28/09
Posts: 711
Loc: USA
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Then why not. If you have the extra time, and arent digging deeper in your pockets, go for it. Im sure it wouldnt hurt any, but how many jars do you typically knock up at a time? Im sure it will get fustrating when you get into the teens+. For me it would
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