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Offlineganjagoa
Stranger
Registered: 12/22/02
Posts: 3
Last seen: 13 years, 11 months
Flaming needle inbetween injections..... PROBLEM
    #1161659 - 12/22/02 08:04 AM (13 years, 11 months ago)

I read that inbetween injections of each jar, you should flame steralize your spore syringe with a alcohol flame.

Wouldn't doing this heat the needle to a temperature that kills bacteria, but also the spores? So my question remains, should you have to wait a few seconds after flaming the needle before you stick it in the grains to inject?

simple, but important quesiton. thanks

everything i say is bull shit


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OfflineMickyFinn
Fuck the DJ
Registered: 12/19/01
Posts: 760
Loc: VA Beach VA
Last seen: 6 years, 9 months
Re: Flaming needle inbetween injections..... PROBLEM [Re: ganjagoa]
    #1161661 - 12/22/02 08:06 AM (13 years, 11 months ago)

keep alcohol handy, swab the needle in between inoculations, works fine


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Offlineganjagoa
Stranger
Registered: 12/22/02
Posts: 3
Last seen: 13 years, 11 months
Re: Flaming needle inbetween injections..... PROBLEM [Re: MickyFinn]
    #1161664 - 12/22/02 08:07 AM (13 years, 11 months ago)

Will the typical 70% isoprol alcohol work for swabing the needle?


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OfflineMickyFinn
Fuck the DJ
Registered: 12/19/01
Posts: 760
Loc: VA Beach VA
Last seen: 6 years, 9 months
Re: Flaming needle inbetween injections..... PROBLEM [Re: ganjagoa]
    #1161666 - 12/22/02 08:09 AM (13 years, 11 months ago)

that'll do


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InvisibleBi0TeK
elephant man

Registered: 11/07/02
Posts: 3,002
Loc: Yorkshire Moors, Great Br...
Re: Flaming needle inbetween injections..... PROBLEM [Re: ganjagoa]
    #1161798 - 12/22/02 09:00 AM (13 years, 11 months ago)

I make my own syringes and always flame my needles with just a lighter and have never run into any problems. I also flame sterilize to pull abit of air into the syringe but swabbing the needle with alchohol works as well so I've heard.


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PROMOTE BACTERIA. THEY'RE THE ONLY CULTURE SOME PEOPLE HAVE.


Edited by Bi0TeK (12/22/02 09:15 AM)


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Offlinemoorhsum
SEED MASTER

Registered: 11/18/02
Posts: 598
Loc: N America
Last seen: 13 years, 2 months
Re: Flaming needle inbetween injections..... PROBLEM [Re: ganjagoa]
    #1161820 - 12/22/02 09:20 AM (13 years, 11 months ago)

Just make sure that theres no peices of substrate like grain or rice flour in the point of your needle, cause sometimes they get plugged, and the stuff inside can rot.


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We are the counter-culturaly elite...


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Anonymous

Re: Flaming needle inbetween injections..... PROBLEM [Re: ganjagoa]
    #1161830 - 12/22/02 09:26 AM (13 years, 11 months ago)

Flame the needle and stick it right in. The air cools it fast, and most of the heat that the air does not remove will dissipate in the substrate.


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OfflineAngry Mycologist
Spontaneouslycombusting

Registered: 11/24/02
Posts: 1,282
Loc: Galapagos
Last seen: 13 years, 7 months
Re: Flaming needle inbetween injections..... PROBLEM [Re: Anonymous]
    #1162058 - 12/22/02 11:57 AM (13 years, 11 months ago)

Yea, once you take the needle out of the flame it cools very quickly. I didn't use rubbing alcohol, just an alcohol flame.


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The proper penalty of ignorance, which is of course that those who don't know should learn from those who do... - Plato


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OfflineMrbadguy
Marquis de Sade

Registered: 12/19/02
Posts: 69
Loc: Enemy territory
Last seen: 1 year, 11 months
Re: Flaming needle inbetween injections..... PROBLEM [Re: ganjagoa]
    #1164088 - 12/23/02 07:51 AM (13 years, 11 months ago)

I didnt have the time and patience to go out and buy alcohol, so i used this hand sanitizer that contained 62% Ethyl Alcohol and Isopropyl alcohol with water and gelatin and i didnt get any contams. im probably just insanely lucky anyway...


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...hypothetically speaking of course.


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Invisibleralphster44
collector
 User Gallery

Registered: 01/04/01
Posts: 4,657
Re: Flaming needle inbetween injections..... PROBLEM [Re: MickyFinn]
    #1164111 - 12/23/02 08:02 AM (13 years, 11 months ago)

Post deleted by administrator.


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For your safety and security, we have a Secure Website.
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OfflineCurious_George
You want abaloon?

Registered: 07/15/02
Posts: 1,065
Loc: La La Land
Last seen: 11 years, 8 months
Re: Flaming needle inbetween injections..... PROBLEM [Re: ralphster44]
    #1164164 - 12/23/02 08:16 AM (13 years, 11 months ago)

Hmm... i sterilize my needle when i start and do all the jars, never had problems, just lucky i guess, but as long as your jars are PC'ed and sterile, things should be cool, cleaning the needle between jars is still a good idea, but overkill i'd think!!

the part about grain stuck inside the needle, well, that grain should be sterile and will only be there for a few seconds until you push it out with more solution, so i see no problems there!

my 0.02 canadian.. so that's what.. 0.015 us?


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************************************.>>>>>
Here Johny,, have a joint.. all your friends are doing it!!


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