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OfflineCray
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The whole flaming the needle..
    #3852074 - 03/01/05 02:52 PM (12 years, 9 months ago)

I got a question about that, when inoculation, each time you take it out of each hole in each lid do you have to flame the needle every time? Or can I just wipe it with rubbing alcohol? Also it is best to do this with every hole injection?

Thanks,
Cray
:peace:


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Edited by Cray (03/01/05 02:55 PM)


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InvisibleNeedMoreSleep
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Re: The whole flaming the needle.. [Re: Cray]
    #3852138 - 03/01/05 03:04 PM (12 years, 9 months ago)

Obviously the safest way would be to flame it and wipe it w/ alcohol before its stuck into any innoc point, same jar or not...  Flaming is for sterilizing the inside hollow portion of the needle, alcohol is to sterilize the outside of the needle and to cool it after flaming... With that knowledge, do what you thinks best for your particular situation... GL :stoned:


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OfflineCray
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Re: The whole flaming the needle.. [Re: NeedMoreSleep]
    #3852172 - 03/01/05 03:12 PM (12 years, 9 months ago)

aww I get it! Is it alright just to use a cigerate lighter? Also when I heat it, do I heat it until it get red hot or just heat it up some? After heating it up, just let it cool down? In some instuctions I've read it's said to squrit a little bit out. I'm doing quite a bit of jars so, whats the best way? (12 jars x 4 holes in each jar = 48 holes)


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OfflineHambo
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Re: The whole flaming the needle.. [Re: NeedMoreSleep]
    #3852177 - 03/01/05 03:12 PM (12 years, 9 months ago)

Flaming it red hot will kill everything inside or out instantly think how hot it must be when it glows.. wiping with alcohol should do.. I mean I can't see too many bacterias climbing up the needle point, but its better safe than sorry eh? not much effort. Flaming followed by alcohol will cool the needle also so you can inoculate again without killing any spores passing through. I do this after every 2-3 of jars with 2 innoc points each.


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OfflineCray
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Re: The whole flaming the needle.. [Re: Hambo]
    #3852215 - 03/01/05 03:22 PM (12 years, 9 months ago)

So would it be better to flame the needle every 2-3 jars, but wipe the needle with alcohol everytime you go to inject in each hole?


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Re: The whole flaming the needle.. [Re: Hambo]
    #3852233 - 03/01/05 03:25 PM (12 years, 9 months ago)

Quote:

Hambo said:
I mean I can't see too many bacterias climbing up the needle point, but its better safe than sorry eh?




If you're doing PF cakes, you've got a layer of verm on top right?  Think about what that layer of verm is for... Its for "catching" contams.  Still don't see bacteria gettin in there when you stick it in there each time????  Obviously it should be sterile at the time of inoculation, but the possibility still exists none-the-less...  Like you said, better safe than sorry :stoned:


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which we must fear most. And that is... the indifference of good men."
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OfflineCray
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Re: The whole flaming the needle.. [Re: NeedMoreSleep]
    #3852243 - 03/01/05 03:28 PM (12 years, 9 months ago)

god da*n I'm getting confused now! wtf, so do I flame the needle EACH time and wipe it? Or what do you suggest NeedMoreSleep?


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InvisibleNeedMoreSleep
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Re: The whole flaming the needle.. [Re: Cray]
    #3852256 - 03/01/05 03:30 PM (12 years, 9 months ago)

flame it, wipe it, innoc, flame it, wipe it, innoc, lather, rinse, repeat... seriously shortcutting it will only save you like 1 minute for a dozen jars man...


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OfflineCray
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Re: The whole flaming the needle.. [Re: NeedMoreSleep]
    #3852275 - 03/01/05 03:35 PM (12 years, 9 months ago)

and do that for each hole?...

Ok think I got it, how long does the jars after steam serilizing take to cool down?


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Offlinethenewguy05
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Re: The whole flaming the needle.. [Re: Cray]
    #3852279 - 03/01/05 03:35 PM (12 years, 9 months ago)

Personaly i just wipe it down as soon as i pop the cap, and if it touches anything i wipe again, and once before i put the needle back. everyone has their own style. do what you think/works best 4 u. As 4 the jars cooling down. MAKE SURE THEY ARE COOL. i just f***ed up my great race between south american and golden teachers by innoculating too early.(I think the center might have been too hot still...I'm Pretty Sure) I waited overnight but I have plastic canning jars. they prolly cool down faster than glass. So i guess i would have to say 12-24 hours. just cool to the touch is the rule of thumb. not much else I can say.


Edited by thenewguy05 (03/01/05 03:47 PM)


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OfflineCray
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Re: The whole flaming the needle.. [Re: thenewguy05]
    #3852287 - 03/01/05 03:37 PM (12 years, 9 months ago)

alright


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OfflineXTCollection
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Re: The whole flaming the needle.. [Re: Cray]
    #3852383 - 03/01/05 04:04 PM (12 years, 9 months ago)

Seriously, flame the syringe only if open and used from a previous batch. If your air has that much bacteria in it, I think its more propable your jars would be contaming.

AFOAF only flames in between batches, alchol wipe in between jars.


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OfflineMages
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Re: The whole flaming the needle.. [Re: XTCollection]
    #3854756 - 03/02/05 12:17 AM (12 years, 9 months ago)

You can flame the needle with a cigarette lighter (I've done it) but it's a pain in the a**. If you're doing it that way, it probably would be easier to flame every few jars and alcohol-wipe every hole. HOWEVER: With any jar-tek your biggest exposure to contamination comes from the inoculation itself. You'd do better to get some little lamp that will keep burning, so it's easy to flame on each hole. Then, flaming before each insertion of the needle is easy and you don't find yourself taking shortcuts you might regret 2 weeks later.

- Mages


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Offlinewds
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Re: The whole flaming the needle.. [Re: Mages]
    #3855096 - 03/02/05 01:49 AM (12 years, 9 months ago)

needmoresleep, whats "OCD"?


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OfflineTomatadunothing
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Re: The whole flaming the needle.. [Re: wds]
    #3855139 - 03/02/05 01:59 AM (12 years, 9 months ago)

Obsessive Compulsive Disorder

As far as the syringes go, I heard a good tip recently that I am yet to try, but it sounds pretty good.

Cut up a clean make up sponge into small sections that just barely cover the needle. Make sure the sponge stays soaked up with Iso. when you're noc'ing up jars, and your needle should stay clean with no extra work.


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OfflineHambo
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Re: The whole flaming the needle.. [Re: NeedMoreSleep]
    #3855413 - 03/02/05 03:10 AM (12 years, 9 months ago)

Quote:

NeedMoreSleep said:
Quote:

Hambo said:
I mean I can't see too many bacterias climbing up the needle point, but its better safe than sorry eh?




If you're doing PF cakes, you've got a layer of verm on top right?  Think about what that layer of verm is for... Its for "catching" contams.  Still don't see bacteria gettin in there when you stick it in there each time????  Obviously it should be sterile at the time of inoculation, but the possibility still exists none-the-less...  Like you said, better safe than sorry :stoned:




Oops, I forgot about that, I don't put a top layer of dry verm see. I put a tyvek filter and sterile plaster over the hole. Thinking about it, aye I could see it being an issue with the regular PF tek.


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InvisibleSHiZNO
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Re: The whole flaming the needle.. [Re: Hambo]
    #3855591 - 03/02/05 04:01 AM (12 years, 9 months ago)

ill either flame heat the needle or wipe with alcohol, between jars, but not between innoc points


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Offlinefrock
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Re: The whole flaming the needle.. [Re: SHiZNO]
    #3856002 - 03/02/05 08:39 AM (12 years, 9 months ago)

I flame every 3 jars and wipe with alcohol between every 2.

I have never had a problem with contams in my jars except 1 time in the last year. It was probably just a bad syringe I made to.

You do not need to wipe between each hole. To me that is overdoing it.

Its really up to you. I usually do like 36 jars at a time, so it would be a pain to wipe between every hole.


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OfflineAnnoA
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Re: The whole flaming the needle.. [Re: frock]
    #3856008 - 03/02/05 08:43 AM (12 years, 9 months ago)

>I flame every 3 jars and wipe with alcohol between every 2.

This is a sound way to do it.
Flaming after each hole or even after each jar is a waste of time.


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Offlinegsmith
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Re: The whole flaming the needle.. [Re: Anno]
    #3856057 - 03/02/05 09:04 AM (12 years, 9 months ago)

anno is right. i've even inoculated a bunch of jars only flaming the needle once before starting and none of them contaminated. as long as the foil stays on the jars before you inject and you don't let the needle touch anything in between jars it should be fine. Unless your air is rife with contaminants, it should be pretty hard for that tiny little needle to pick up a sufficient ammount of particles to cause a problem. IME the source of contamination from inoculation is usually inside the syringe, not from the air.


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