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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Pressure Cooker, Vermiculite

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Offlinedareworked4me
Stranger
Registered: 11/01/00
Posts: 2
Last seen: 15 years, 3 months
help!! major contamination problems
    #527676 - 01/21/02 10:50 PM (15 years, 3 months ago)

i have been growing cubensis for three years and just recently, i have had big, big problems with green mold. First i clean and wipe down the counter and around the room. I then use a mixture of 4 cups coarse ground verm and 1 cup brown rice flour and 1 cup water. I use a pressure cooker and cook the jars at 15 psi for 40 min. I then sterilize the spore syringe needle with a lighter and inject cakes with spores. In between cakes, i use a cotton swab with rubbing alcohol to clean the needle. no matter what i do, mold will grow top to bottom, and occasionally where the needle injected spores. I have got brand new syringes and sterilized them and STILL have problems. after making something like 50 cakes, only a pathetic 5 are lucky enough to survive. I am just about ready to throw in the towel becuase no matter what i've done, nothing seems to work. . . HELP!!!!

p.s. I also have problems with my recepie for substrate causing the mixture to become a little mucky and slow down myceluim growth.


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Offlinefresh357
addict

Registered: 09/02/01
Posts: 493
Last seen: 15 years, 17 days
Re: help!! major contamination problems [Re: dareworked4me]
    #527694 - 01/21/02 11:10 PM (15 years, 3 months ago)

well I can give you two more things to help cut down contams, the oven tek and lysol...other than that your procedure is sound from what i can see.

Also..who is your spore supplier? If they are all from the same source then it might not be your fault at all.


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OfflineCrabTree
newbie
Registered: 01/20/02
Posts: 37
Loc: Iceland
Last seen: 15 years, 1 month
Re: help!! major contamination problems [Re: dareworked4me]
    #527705 - 01/21/02 11:20 PM (15 years, 3 months ago)

Maybe this will help, Im sure you know most of the teks and you probably know everything i will say, but Maybe you can use it as a double check list.

1. Some teks suggest pulling air into the syringe so spores can be shaken. But pulling room temperature air can and probably does contain other bacteria and other spores. Which will contaminate the entire syringe

2. Try using polytek, or another filter of some sort.

3. Keep the jars in a sterile environment if possible

4. Keep the substrate from being too watery. If you can squeeze water from it, it's probably too wet. wet substrate attracts molds

5. use a top 1/2 inch of dry verm ontop of substrate to further protect against contams

Hope this helps..


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Invisiblephrozendata
Carpal Tunnel

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Registered: 04/24/00
Posts: 5,015
Re: help!! major contamination problems [Re: CrabTree]
    #527727 - 01/21/02 11:55 PM (15 years, 3 months ago)

Make sure that you are letting the jars slowly cool while still sealed, tightly, in the pressure cooker. I usually just turn off the heat and walk away. A good 3 hours later and i'll take off the pressure cooker lid and tighted the jar lids, then close them in the pressure cooker again, and then innoculate them 2 or 3 hours later. I've also had success with waiting untill the jars are fully cooled down, taking them out, then tightening them and then innoculating.

Hope that helps.


--------------------
"There's only one corner of the universe you can be certain of improving and that's your own self. So you have to begin there, not
outside, not on other people" - Aldous Huxley


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InvisibleWakingUpLate
addict
Registered: 12/29/01
Posts: 559
Loc: Born on a mountain, Raise...
Re: help!! major contamination problems [Re: dareworked4me]
    #527748 - 01/22/02 12:21 AM (15 years, 3 months ago)

This may be overkill but here's a few ideas:

Before you mix your substrate:
Presteralize your substrate 20min/300deg in a
corningware/pyrex bowl w/ lid.
Do the same with your verm about twice as
long, you can throw in a couple of spoons,
knives, pie server ect with metal handels to
work with.
PC your jars 20 min and Boil your water.
Do this where it all comes out at about the
same time. Take the stuff out of the oven
and set them outside(covered) In 30min to
an hour it will all be cool enough to handle
(given it's cold outside). Wash your hands
with anti-bactirial soap and go for it.
Put your flour and verm in new ziplocks and
use them as you need them.
Before you innoculate give your jars/tape a
quick rubdown with alchohol.
When you receive a shippment of goodies from
your supplier. Resist the urge to open it right away.
It was probably packed in a semi-sterile enviorment
but no telling what's on the outside of it. Wash your
hands good, handle the package as little as possible
to get it open, and place your syringes/prints ect itno
a big ziplock until you are ready to use them. Then
you can look at them all you want.
During the (somewhere around) two week process
of watching cakes colonize, there is only one or two
days that you really effect what's going on inside.

Like I said it may be overkill, but, I have an eighty lb.
pit bull in the house and haven't lost a jar to contams
yet. Hope that helps! Good luck!


--------------------
The rest of those, who have gone before us,
cannot settle the unrest of those who follow.
(Finding Forrester)


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Offlinetomldp
Shroomeur

Registered: 10/03/01
Posts: 389
Loc: Europe
Last seen: 13 years, 5 months
Re: help!! major contamination problems [Re: WakingUpLate]
    #527892 - 01/22/02 03:43 AM (15 years, 3 months ago)

Work on the substrate, it should not be muddy. Each time I prepared a muddy substrate, i had troubles.


--------------------
:laugh: Visit and support the Free Spore Ring Europe :laugh:


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Offlinedareworked4me
Stranger
Registered: 11/01/00
Posts: 2
Last seen: 15 years, 3 months
Re: help!! major contamination problems [Re: tomldp]
    #528469 - 01/22/02 08:11 PM (15 years, 3 months ago)

hey guys thanks a lot for the replies. I will try a few new things that you suggest. I'll keep you posted on any luck in the future.


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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Pressure Cooker, Vermiculite

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