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OfflineMr_zebra
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Registered: 08/22/02
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non sterile spore prints
    #998584 - 10/28/02 12:25 AM (14 years, 7 months ago)

I want to use honey water or cardboard plus honey water . whats the deal with this ? how do i use prints from something I know isnt sterile ? this isnt covered in the faq it all assumes I am printing from indoor sterile-ness. thanks.


Edited by Mr_zebra (10/28/02 12:53 AM)


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OfflineXiC_clone
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Re: non sterile spore prints [Re: Mr_zebra]
    #998594 - 10/28/02 12:29 AM (14 years, 7 months ago)

hmmm?


-XiC


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OfflineIcy
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Re: non sterile spore prints [Re: Mr_zebra]
    #998624 - 10/28/02 12:39 AM (14 years, 7 months ago)

Do you mean you picked a mushroom outside and printed it, so you think it's not sterile? or what?


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InvisibleSixTango
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Registered: 01/21/02
Posts: 1,996
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Re: non sterile spore prints [Re: Mr_zebra]
    #998632 - 10/28/02 12:41 AM (14 years, 7 months ago)

Spores from a non/sterile print, going into honey water will generly get you a jar of grungy liquid contams.

About the only thing to gain sterile material is go to agar with it & isolate some clean material as it colonizes the plate. Arag is easy, but not exactly a beginner thing - either. Because, impart -=- you should do that in a glove box or under a flow hood.

6T  :tongue:


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OfflineMr_zebra
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Re: non sterile spore prints [Re: SixTango]
    #998649 - 10/28/02 12:46 AM (14 years, 7 months ago)

Thanks for all of the replies. I have heard that beer(boiled) and cardboard has been substituted for agar . Can i just go with honey water and cardboard ?


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OfflineMr_zebra
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Re: non sterile spore prints [Re: Mr_zebra]
    #998821 - 10/28/02 01:47 AM (14 years, 7 months ago)

Here is what i plan to do :


    Add honey water to cardboard
    Microwave or pressure cook -- is this nessary since i wont be starting sterile at all ?
    Add spores
    Once myclial growth starts add some kind of diluted bleach or h202
    slice out a good rhizomorphic section


Sounds sound ?


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OfflineChromeCrow
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Re: non sterile spore prints [Re: Mr_zebra]
    #998913 - 10/28/02 02:30 AM (14 years, 7 months ago)

dont even waste your time on honey water, just adds another way to contam , just use straight cardboard, soaked in a small dish of distilled water, and nuked in a microwave for about 5 - 7 minutes. the cardbaord wont contam since there is no nutrients for the lil buggers to eat, but spores will germintate great on it. let it grow some, and drop it on your grain, or make a myc suspension with it. if your going to sector, it would be wise to use agar.


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OfflineMr_zebra
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Re: non sterile spore prints [Re: ChromeCrow]
    #998936 - 10/28/02 02:52 AM (14 years, 7 months ago)

thanks chrome that was very helpful
let me review to make sure everything is clear as if i were reading your order back to you because you might not want onions on that

    Add distilied water to cardboard
    Microwave or pressure cook -- is this nessary since I wont be starting sterile at all ?
    Add spores -- if I dont need to microwave I'll just print them on the card board
    Once myclial growth starts add some kind of diluted bleach or h202 --which is better ?
    slice out a good rhizomorphic section


and I assume we are talking about corregated cardboard


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OfflineISH
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Re: non sterile spore prints [Re: Mr_zebra]
    #1294532 - 02/09/03 08:30 PM (14 years, 4 months ago)

I want to try this with Pan cyan, it's a fast colonizer.
Peace,
ISH


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InvisibleZen Peddler
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Re: non sterile spore prints [Re: ISH]
    #1294666 - 02/09/03 09:36 PM (14 years, 4 months ago)

You want to use wild prints then youll need to use agar - end of story.


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OfflineAnnoA
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Re: non sterile spore prints [Re: Zen Peddler]
    #1294697 - 02/09/03 09:56 PM (14 years, 4 months ago)

Hmmm...

I?ve been using a different method on various occasions, and it worked well.

I make a fairly dilutes spores suspension and inoculate a classic PF cake with it on 4 spots with little inoculant.

One or more of the spots usually show clean growth, then you can transfer those spots to either peroxided agar, or directly to peroxided grain.



Edited by Anno (02/09/03 09:58 PM)


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