Home | Community | Message Board


Gaiana.nl
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: ½ Pint Jars

Jump to first unread post. Pages: 1
OfflineGr8fulJ420
strange but nota stranger
Male User Gallery

Registered: 02/18/01
Posts: 2,778
Loc: 0 moco
Last seen: 9 months, 23 days
Frozen syringes... ooops!
    #943398 - 10/08/02 05:56 PM (14 years, 7 months ago)

Moved into a new place a few months ago. While moving in, my roommate accidently stashed two syringes in the freezer instead of the refrigerator like I asked. They were probably in there for a few weeks before I noticed and took them out. Will the spores still be viable or should I invest in some new samples?


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinerosewoodpete
a grain of sandamongst thedunes

Registered: 05/11/02
Posts: 1,481
Last seen: 3 years, 8 months
Re: Frozen syringes... ooops! [Re: Gr8fulJ420]
    #943543 - 10/08/02 06:56 PM (14 years, 7 months ago)

i'm not sure but your best bet would be to have let them thaw slowly in the frig rather than shock them with room temp.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleCow Shit Collector
Patty Poacher

Registered: 02/15/01
Posts: 1,959
Loc: Random Field
Re: Frozen syringes... ooops! [Re: Gr8fulJ420]
    #943571 - 10/08/02 07:04 PM (14 years, 7 months ago)

were the syringes frozen solid? If so, i'm gonna say that they are done. But try in 2 jars to see I'm not sure if spores survive freezing. I'm pretty sure mycelium survives but spores seem to be more fragile...


--------------------
_______________________________________
CSC


Life's a garden, Dig it!
~Joe Dirt

Off Topic Website


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineGr8fulJ420
strange but nota stranger
Male User Gallery

Registered: 02/18/01
Posts: 2,778
Loc: 0 moco
Last seen: 9 months, 23 days
Re: Frozen syringes... ooops! [Re: Cow Shit Collector]
    #943622 - 10/08/02 07:21 PM (14 years, 7 months ago)

They were frozen solid. And i returned them to the fridge to thaw slowly. I made up a batch of PF-style jars last night and was just wondering what my chance of success was.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleCow Shit Collector
Patty Poacher

Registered: 02/15/01
Posts: 1,959
Loc: Random Field
Re: Frozen syringes... ooops! [Re: Gr8fulJ420]
    #943707 - 10/08/02 07:48 PM (14 years, 7 months ago)

i'd say not to good, but i dont want to be a pessimest


--------------------
_______________________________________
CSC


Life's a garden, Dig it!
~Joe Dirt

Off Topic Website


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleZwieback0
Baby Bread
 User Gallery

Registered: 08/23/02
Posts: 3,473
Re: Frozen syringes... ooops! [Re: Cow Shit Collector]
    #943817 - 10/08/02 08:39 PM (14 years, 7 months ago)

Dead  :frown: 


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblefailbot999
Registered: 02/18/02
Posts: 590
Re: Frozen syringes... ooops! *DELETED* [Re: Zwieback0]
    #943828 - 10/08/02 08:43 PM (14 years, 7 months ago)

Post deleted by ski_stoned

Reason for deletion: delete



Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblematts
matts

Registered: 01/28/02
Posts: 3,649
[Re: failbot999]
    #943994 - 10/08/02 09:50 PM (14 years, 7 months ago)



Post Extras: Print Post  Remind Me! Notify Moderator
OfflineFeelers
Anti-Myth-Rhythm-Rock-Shocker
Male User Gallery

Registered: 06/19/02
Posts: 1,798
Loc: Land of Oz
Last seen: 3 hours, 30 minutes
Re: Frozen syringes... ooops! [Re: matts]
    #944104 - 10/08/02 10:41 PM (14 years, 7 months ago)

Since there was probably millions of spores in there i'd geuss that at least some would survive, when sperm is frozen half of it dies, but it doesnt really matter coz there are so many of the buggers. On the other hand the spores might get a lil funked, but hey, give it a shot, its not worth throwin em out without giving it a go. Let us know if it works.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineGr8fulJ420
strange but nota stranger
Male User Gallery

Registered: 02/18/01
Posts: 2,778
Loc: 0 moco
Last seen: 9 months, 23 days
Update: Frozen syringes... ooops! [Re: Feelers]
    #961481 - 10/15/02 01:06 AM (14 years, 7 months ago)

Last Thursday (10/9?) I innoculated one dozen jars with the spore solution that had been frozen. With nothing to lose I went for broke and put 1 to 1.5 cc in each injection spot. Put the jars back in their cardboard box, but the box on top of my stereo receiver (~73-76ish F), and went out of town for the weekend. Checked them last night and there are quarter-sized gray patches where I injected the solution.

Hopefully things will continue well! I am going to make a slightly better incubator to keep the temp up a little, now that I think they will work.


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: ½ Pint Jars

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* frozen spore syringes frank_grimes 1,775 3 06/18/05 02:02 AM
by Ryche Hawk
* Frozen Syringes --- Fibers floating after thaw DewDrop 639 12 08/31/08 09:07 PM
by alkylbenzene23
* Frozen Spores? soko 2,038 12 02/04/17 03:26 PM
by Roflspammer
* frozen spore syring pipeDream 4,015 8 01/24/14 04:50 PM
by Fungal growth
* frozen syringes...still good? Elfmachine 600 4 01/18/05 01:39 AM
by Elfmachine
* Frozen Spore Syringes!!!! shr00matik 1,360 3 07/12/07 03:36 AM
by Anno
* frozen syringe....bad? multisonic 889 9 01/16/08 02:38 AM
by Gr8fulJ420
* Frozen Liquid Mycelium Culture flavoraid 1,543 13 03/12/08 06:04 PM
by flavoraid

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, RogerRabbit, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte
889 topic views. 7 members, 73 guests and 18 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Original Seeds Store - Cannabis Seeds
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.032 seconds spending 0.008 seconds on 18 queries.