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Registered: 08/29/04
Posts: 234
Will frozen substrate fruit once thawed out?
    #3551848 - 12/28/04 09:40 AM (12 years, 3 months ago)

Say you want to make large amounts of spawn/subtrate for making casings weeks or possibly months down the road.

Does anyone know if the myc would die in the freezer and if it would still fruit after being thawed out again?

Or would it be better to keep it in the fridge until ready for use?

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Registered: 09/14/04
Posts: 560
Re: Will frozen substrate fruit once thawed out? [Re: madsickpenguin]
    #3551932 - 12/28/04 10:29 AM (12 years, 3 months ago)

I would keep it in the fridge, that will slow down growth. I think the freezer would kill the mycelium. That's why cubies aren't found up north, the myc doesn't survive the winter cold.

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Registered: 09/30/04
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Re: Will frozen substrate fruit once thawed out? [Re: shobimono]
    #3551960 - 12/28/04 11:05 AM (12 years, 3 months ago)

The only exceptions would be... (Doubt that you have the tools to do it.)

Freeze drying with liquid nitrogen. You would have to freeze it so fast that the water inside would not expand. (Slow freezing causes water to link into hoola-hoops, placing excess stress on the subject in question from the inside-out. (That is why it apears to expand, and creates air bubbles.)


Dehydrate it with cold air, then slowly freeze what remains. This removes most of the damaging water from the inner structure of the cells. However, you risk the chance of killing the culture this way. Once rehydrated, they will carry on as normal, if they survive.

Mycelium DOES survive the cold up here, I have pulled fresh mushrooms from trees and the ground in 20F weather. However, these mushrooms will not survive, as they are not adept to these harsher environments, as they contain too much water internally. Mushrooms and mycelium don't thrive all winter, but they don't all die!

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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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