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InvisibleDickhead
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Frozen Liquid Culture
    #5948700 - 08/09/06 08:40 PM (17 years, 5 months ago)

I usually toss a used LC.

This time after a healthy inoculation of some successful cakes I decided to stick it in the freezer.

It's frozen solid. In a day or so I'm going to thaw it and inoculate a few to see how it goes.

I've read about mycelium surviving freezing, nice if it works!


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OfflineGreenScreen
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Registered: 07/02/06
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Re: Frozen Liquid Culture [Re: Dickhead]
    #5948726 - 08/09/06 08:48 PM (17 years, 5 months ago)

i doubt it will still be viable but you can give it a shot

let us know how it turns out. good luck


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OfflineRogerRabbitM
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Re: Frozen Liquid Culture [Re: GreenScreen]
    #5948790 - 08/09/06 09:07 PM (17 years, 5 months ago)

If it doesn't survive, try it next time after putting a popsicle stick or tongue depressor, etc. in with the LC when you make it. The myc will begin to colonize the wood, and I believe that might help it survive. It's how oyster mushrooms in dead trees survive the winter. I use a piece of wood in all my test tube slants, and store them at 35F/+2C.
RR


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