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I have heard of people using ozone. One problem, it corrodes everything. We used to use it for our hot tub. And it degraded everything in the room, plastic, wood, styrofoam etc. So only do it if you don't mind things desintegrating. The other problem is that you have to have it running all the time to be effective. You would also most likely have to have a vestibule so that when you entered the room you didn't flood it with contaminants.
A glove box is a hell of a lot cheaper/easier and doesn't require a sterile room.
Hehe, not really, I just like making cool things, that and I wanted to start doing strain selection and didn't trust the cleanest room in my apartment - a musty closet. (I cleaned it three times! I swear!)
That..AND, I can be fairly sure of a wonderfully minimal contamination rate in anything sensitive. (well, contams gained at the time of innoculation)
[This message has been edited by Ultrapope (edited February 20, 2000).]
I don't think it would be required to run the generator the whole time anything sensitive is in the box. Ozone's pretty unstable, and I bet a few minutes after flooding the box with it, most of it would have dissipated by oxidizing everything in sight. One good charge of ozone should make the whole box sterile, provided that there is extremely minimal airflow (hehe, I could fill my box with water and it wouldn't leak...in fact...I did
I haen't tested it out yet. I was a younger man when I got involved (anybody remember oss and oeric? Psilly Psimon? Mescalito Ted?) and all my quip got junked by a horrified mom and dad.
Hehe...boy were they pissed
Give me a few weeks, then I'll experiment with every whim that I can come up with
"If it's not nailed down, it's free" - Captain Crapp