|
 Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
 World Spirit
PNW
Registered: 07/27/01
Posts: 9,817
|
 Re: How to make a glove box cheap & easy  [Re: Beatnik]
#525459 - 01/19/02 10:22 AM (21 years, 10 months ago) |
|
|
Deleted by admin
|
 SThomas
enthusiast
Registered: 10/15/01
Posts: 251
Loc: Ottawa, Ontario
Last seen: 21 years, 6 months
|
 Re: How to make a glove box cheap & easy [Re: World Spirit]
#526204 - 01/20/02 01:21 AM (21 years, 10 months ago) |
|
|
Just my two cents at the end of this long post...but I say screw glove boxes. I work under less than optimum sterile conditions with my agar....zero contams after making a dozen plates, and just to be on the safe side I mix in H2o2 in with my new agar batches to be colonized by new mycelium slices, works like a charm and a lot less effort. Who knows I may be just a lucky sob...but it works great....cheers
--------------------
And that is all the news that's fit to print
|
World Spirit
PNW
Registered: 07/27/01
Posts: 9,817
|
 Re: How to make a glove box cheap & easy [Re: SThomas]
#526240 - 01/20/02 02:02 AM (21 years, 10 months ago) |
|
|
Deleted by admin
|
SThomas
enthusiast
Registered: 10/15/01
Posts: 251
Loc: Ottawa, Ontario
Last seen: 21 years, 6 months
|
Re: How to make a glove box cheap & easy [Re: World Spirit]
#526504 - 01/20/02 11:28 AM (21 years, 10 months ago) |
|
|
Hehe...I knew that would come up. I don't say that I am super experienced when it comes to agar, and I do expect to have some contams eventually, I just think that it is a lot of work to build these things...of course if I had a tonne of money I would probably buy a pro sterile room for myself. But the way I figure it, getting the odd agra contam, is worth not having to go through the hassle of major construction efforts...hehe. That is the great thing about agar, you know pretty soon if you have contams, and then you can either throw the plate away, or cut a nice piece of mycelium off and start a new plate buffered with H2o2...just my two cents though, I do applaud anyone who can construct a flow hood or glove box...and have good results with it. Cheers
--------------------
And that is all the news that's fit to print
|
oscill8
*bondage fairy*
Registered: 02/26/01
Posts: 1,215
|
 Re: How to make a glove box cheap & easy [Re: SThomas]
#526610 - 01/20/02 02:04 PM (21 years, 10 months ago) |
|
|
the problem with peroxidated agar is that you cannot start spores from it. people working with "questionable" prints cannot rely on peroxide- you would either have to start from a live culture (which are hard to come by, unless someone is willing to send you one), or a piece of a mushroom. so, great for cloning and transfers, but the print thing is a drawback. gluck on no contams, i know my house if full of em. youre lucky :)
enter- congrats on the glovebox. its always extremely satisfying to build something yourself and use it :) hope it serves you well, at least til you upgrade to the cadillac :)
--------------------
? oscill8 2001, 2002
"Any data submitted to Shroomery.org becomes our property"
|
World Spirit
PNW
Registered: 07/27/01
Posts: 9,817
|
Re: How to make a glove box cheap & easy [Re: oscill8]
#526615 - 01/20/02 02:16 PM (21 years, 10 months ago) |
|
|
Deleted by admin
|
My Third Eye
old hand
Registered: 11/02/00
Posts: 641
|
Re: How to make a glove box cheap & easy [Re: FatSean]
#526643 - 01/20/02 02:43 PM (21 years, 10 months ago) |
|
|
i think the problem with carboard glove boxes are that they soak up moisture wich could breed contams in the cardboard.thats just my opinion because i used a cardboard box for a while then i switched to a rubbermaid container with some heavy duty rubber gloves and had a lot less contam problems.
--------------------
suddenly a flaming stealth banana split the sky
like one would hope but never really expect
to see in a place like this.
Cutting right angle donuts on a dime
and stopping right at my Birkenstocks,
and me yelping...Holy fucking shit!
|
World Spirit
PNW
Registered: 07/27/01
Posts: 9,817
|
Re: How to make a glove box cheap & easy [Re: My Third Eye]
#526646 - 01/20/02 02:47 PM (21 years, 10 months ago) |
|
|
Deleted by admin
|
My Third Eye
old hand
Registered: 11/02/00
Posts: 641
|
Re: How to make a glove box cheap & easy [Re: World Spirit]
#526711 - 01/20/02 04:07 PM (21 years, 10 months ago) |
|
|
i am absolutely inexperienced with agar at this point..ive got some agar and all the shit to do it with just havnt gotten off of my lazy ass..
--------------------
suddenly a flaming stealth banana split the sky
like one would hope but never really expect
to see in a place like this.
Cutting right angle donuts on a dime
and stopping right at my Birkenstocks,
and me yelping...Holy fucking shit!
|
World Spirit
PNW
Registered: 07/27/01
Posts: 9,817
|
Re: How to make a glove box cheap & easy [Re: My Third Eye]
#526718 - 01/20/02 04:17 PM (21 years, 10 months ago) |
|
|
Deleted by admin
Edited by enter (01/20/02 04:19 PM)
|
duss69
Stranger
Registered: 11/27/01
Posts: 71
Last seen: 17 years, 8 months
|
Re: How to make a glove box cheap & easy [Re: World Spirit]
#527177 - 01/21/02 08:12 AM (21 years, 10 months ago) |
|
|
LO
I'm new to agar work and i started without a glovebox 4 days ago.
I took all the precaution to be sterile all the way.
I innoculated the dishes over my gas.(Which is way sufficient for innoculating jars...)in a lysoled area with clean
body (shower) and all.
The dishes were open for at most 20 seconds each and are
stored in an ultra-lysoled rubbermaid.
And i already can tell you that contams are on their way.
On 5 dishes, 2 are surely contamed and 1 maybe.
The rest looks fine.
In conclusion i decided to build a glovebox to decrease the
risk of contams.
Also because it will simplify my sterile process for all my
innoculation now.(jars, grain to grain...) an d because i like to
build my own tools.
It cost me 20$ for the rubbermaid, the gloves, metal rings to
let hands go throught and glue.
I also bought an HEPA filter for vaccum cleaner (20$) that i'll
fix with a fan (free from an old PC )to create positive
pressure inside the box.
The box is not completly air tight but i'm working on it.
Wish i could post a pic....
It took me maybe 1 hour of work +time for glue to dry.
I havent tested it yet but it will be soon.
Cheers
Duss69
--------------------
Support the FSRE
|
World Spirit
PNW
Registered: 07/27/01
Posts: 9,817
|
Re: How to make a glove box cheap & easy [Re: duss69]
#527209 - 01/21/02 08:56 AM (21 years, 10 months ago) |
|
|
Deleted by admin
|
duss69
Stranger
Registered: 11/27/01
Posts: 71
Last seen: 17 years, 8 months
|
Re: How to make a glove box cheap & easy [Re: World Spirit]
#527565 - 01/21/02 06:12 PM (21 years, 10 months ago) |
|
|
 (( I got no cam.....
Maybe i'll manage to borrow a webcam from someone i know but this will be crappy pics....
I promise to show it to you  )
++
Duss69
--------------------
Support the FSRE
|
My Third Eye
old hand
Registered: 11/02/00
Posts: 641
|
Re: How to make a glove box cheap & easy [Re: World Spirit]
#527593 - 01/21/02 06:43 PM (21 years, 10 months ago) |
|
|
making spore syringes
--------------------
suddenly a flaming stealth banana split the sky
like one would hope but never really expect
to see in a place like this.
Cutting right angle donuts on a dime
and stopping right at my Birkenstocks,
and me yelping...Holy fucking shit!
|
cookiewhore
enthusiast
Registered: 01/03/02
Posts: 385
Last seen: 21 years, 7 months
|
Re: How to make a glove box cheap & easy [Re: biggwigg]
#529381 - 01/23/02 06:52 PM (21 years, 10 months ago) |
|
|
NICE. your dryer vents just gave me the idea to put it on my own. I made my glove box from a long (about 6', and about 2' high) rubber maid container.. the sides are see through.. and for the top, i used plastic sealed on with bathroom calk.
in the side, there is a hepa filter which was on sale at walmart 59.99, this is sealed around with calk as well.
a flap in the end is where things are put in, and two arm holes are cut.
|
|
Threaded
Previous
Index
Next
Red Vein Kratom 
Buy Kratom Extract 
Injection Grain Bag 
Liquid Cultures
Edit 
Reply 
Anonymous
nihilist
Dcyfr
Gr0wer
SixTango
1 2 all )
themills