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Well, when you sterilized the manure was it already inside the plastic containers or did you move it into them afterwards? When you inoculated the manure with the grain spawn, did you let any outside air into the plastic containers? And you say these containers weren't air tight..... I think I know what your problems were.
Next time you may want to sterilize the manure in jars, and either use the oven tek, a flow hood or a glove box to inoculate them. Filter disks allow the substrate to lose moisture, so be sure and take that into account. The chances of contamination depend solely upon your method.
>When you sterilized the manure was it >already inside the plastic containers or >did you move it into them afterwards?
I moved it into them afterwards. (They would have melted.) I first cleaned the plastic containers with liquid dishwashing detergent. When I put the shit in the containers, I was wearing latex gloves that I had drenched in rubbing alcohol and then allowed to dry completely in the flow hood. I also used a large spoon that was treated with alcohol the same way.
>When you inoculated the manure with the >grain spawn, did you let any outside air >into the plastic containers?
I did it in a flow hood.
>And you say these containers weren't air >tight..... I think I know what your >problems were.
That the shit got contaminated after it was put into the plastic containers? Gotta be - but why do so many people not have problems with contamination when they are using containers that aren't airtight? Could it be because they live in areas with low humidity and low spore counts?
>Next time you may want to sterilize the >manure in jars...
>... and either use the oven tek, a flow >hood or a glove box to inoculate them.
Like I said, I used a flow hood.
Can anybody suggest a tek to avoid contaminating the shit?
nest time use bleach to sterilize jars. let them dry upside down on something like a paper towel that has been lightly misted with a lysol or bleach/water mix. sorry, no guarantees but i think this might help.
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