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OfflinePrimate
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Registered: 08/13/99
Posts: 855
Last seen: 11 years, 9 months
using colonized cake to innoculate.
    #905440 - 09/24/02 09:00 PM (18 years, 10 months ago)

I was just going over "Captains Oven Technique," and it mentioned that by using the oven steriization tek you could keep it sterile enough to innoculate jars with with pieces of fully colonized cakes. I was wondering if anyone has done this? and how it worked out. Also how exactly they did it would be cool.

Also i thought i remembered hearing that after doing this with spawn a few times your strain will get messed up. any know if that is so?

Thanks guys

-Primate


--------------------
"Power to the peaceful"

-Micheal Franti and Spearhead


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OfflinePrimate
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Last seen: 11 years, 9 months
Re: using colonized cake to innoculate. [Re: Primate]
    #906385 - 09/25/02 04:04 AM (18 years, 10 months ago)

bump


--------------------
"Power to the peaceful"

-Micheal Franti and Spearhead


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Invisiblemickey_rourke
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Registered: 07/18/02
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Loc: Playa del Carmen
Re: using colonized cake to innoculate. [Re: Primate]
    #906392 - 09/25/02 04:06 AM (18 years, 10 months ago)

This is just like a grain to grain transfer. I do G2Gs all the time using the oven. Wear a shower cap, dust mask, and gloves. Wipe everything down with alcohol and spray the air heavily with Lysol. Be sure and turn off any climate control or anything that moves the air. Turn the oven on low. Do your work inside the oven and work quickly.

Good luck...


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"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five


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OfflinePrimate
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Registered: 08/13/99
Posts: 855
Last seen: 11 years, 9 months
Re: using colonized cake to innoculate. [Re: Primate]
    #907830 - 09/25/02 07:33 PM (18 years, 10 months ago)

does anyone happen to know if there is a guide on specifics of grain to grain trnasfer somewhere online(didn't see one in shroomery archives) Or it would be helpful if anyone that has done it would give me a rundown of the procedure followed.

Basicly i am trying to find out which way is the easiest/best/lowest contam risk.....basicly i am split between doing a mycelium syringe or grain to grain transfer. Any comments or suggests welcome from anyone who has done both. Also i had considered maybe making some liquid agar for innoculation.

Thanks all.

-Primate


--------------------
"Power to the peaceful"

-Micheal Franti and Spearhead


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Offlinecomario
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Registered: 08/03/02
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Loc: U.S.A. (N.E.) & South Europe
Last seen: 18 years, 9 months
Re: using colonized cake to innoculate. [Re: Primate]
    #907881 - 09/25/02 07:56 PM (18 years, 10 months ago)

try both + use common sense = it should work!


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