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Anonymous

Re: Moving a Cake to Substrate
    #91426 - 12/29/99 03:28 PM (16 years, 11 months ago)

I wouldn't try that, because of high risk that cakes has some bacteria on it that could grow when given a chance.

Unless you know how to sterilize a cake.. Maybe if you dig a piece out of the middle nice and clean? That could be interesting.

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Offlinesutec
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Re: Moving a Cake to Substrate
    #91428 - 12/29/99 04:16 PM (16 years, 11 months ago)

Sure you do that. I wouldn't recommend it though, but yes you can. This is essentially just a mycelium innoculation instead of multi-spore germination. However you cannot sterilize the mycelium as trying to do so would almost definantly result in the destruction of the mycelium.

There are a couple of better methods that I would use in place of this however. Check it.

1. Don't wait for your substrate to fruit. Once it is 100% colonized then you can be pretty sure that the mycelium is virile and thriving. Let's say you have 11 out of 12 1/2pt jars that survived (we'll subtract one due to possible contamination). I would use the other 10 jars exactly as you were planning on (birthing/casing) and reserve one jar. Keep this jar in a cool environment until needed, this is now called a "Spawn Master" (I wouldn't store it longer than a week to ensure 100% effectiveness, although in theory you should be able to store them for months under proper conditions). Now after you have birthed/cased the other 10 jars and thoroughly cleaned the contaminated one, sterilize them and prepare another batch the exact way. Except this time don't use spores. Use substrate from your "Spawn Master". Use about 1/10th of the Master per jar. Place them in the incubator and wait. You should notice growth in a couple of days. Once these jars are 100%, repeat, saving one jar and starting again.

2. If you are already too late in the game for this to be a viable option, don't fret man, we gots ya covered :P So you've already cased/birthed all of that beautiful mycelium? No problem, once you have mature fruits prepare another batch of jars, take a nice big/healthy looking shroom, and in a sterile environment and using sterile tools slice open the stem of the mushroom lengthwise (one big long cut up and down the middle of the stem). Now using a sterilized scalpel/knife cut a small chunk of shroom out and place it in your jar, repeat for the rest of your jars. Put them in incubatin' conditions and wait! When they are colonized, I would use step 1. as it's a bit simpler.

If you do this you can successfully shed about a week from your total production times. Doing this essentially bypasses spore germination and established a healthy myclial "core" fast, and that helps to beat out would-be contaminators.

Hope this answers your question and helps!



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Anonymous

Re: Moving a Cake to Substrate
    #91429 - 12/29/99 06:04 PM (16 years, 11 months ago)

Thanx, that really lets me know alot! I appreciate the assistance. BTW: Incubation,.....yer talking about in the jar part or the terranium part? Thanx.


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Anonymous

Re: Moving a Cake to Substrate
    #91430 - 12/29/99 06:07 PM (16 years, 11 months ago)

Also, when you talk about cutting open the stem in mehtod 2. Do you mean, cut out a piece from the inside of the stem? or Just cut it lenghtwise, then chop it end to end - Now having like a stem thats cut from head to toe lenghtwise?

Thanx again.



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Offlinesutec
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Re: Moving a Cake to Substrate
    #91431 - 12/29/99 07:32 PM (16 years, 11 months ago)

Incubation is the jar part :P Incubation is the period of time when your Mycelium is taking hold and colonizing.


As for the cutting of the mushroom the actual technique is to take the stem (all of this should be done in a sterile environment) and cut a little bit into it (1/16 of an inch?) in several pieces and then "peel" the "skin" of the stem, you should be left with the exposed inside of the mushroom. The exposed part is what you want to use to transfer, not the outside (too big of a risk of contamination). If you go under the Grow/Find section and look at the 9er TEK and look through it , it has pictures and very complete instructions on how to accomplish this. Go here: http://www.shroomery.org/9er/Cloning.html

Enjoy!



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Anonymous

Re: Moving a Cake to Substrate
    #91432 - 12/29/99 07:45 PM (16 years, 11 months ago)

I read the directions on what your talking about. But the part that I am having trouble with is, I cannot remove the cake from the jar once the sterile process (pressure cooker) part has been completed. Due to contamination. Now How would I be able to put in the piece of "master" mushroom into new jars of substrate? without taking out some of the substrate to bury it in there?

Or do I just lay it on top or something. Thanx again for your help. Sorry if I seem retarded or something.



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Offlinesutec
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Re: Moving a Cake to Substrate
    #91433 - 12/30/99 12:38 AM (16 years, 11 months ago)

You're correct you don't want to remove the cake, I'd just fill the jars a little less and leave space for your inoculant (whether it is froma spawn master or shroom), then in a sterile environment (I find spraying Lysol right before doing any work is a big help in sterility), slowly open the jar (move as little air as possible) and transfer your spawn/mushroom to the jar. I would personally shake the jar after innoculation to spread the mycelium around a bit.


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