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OfflineGlacius
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PAranoid:Oven tech mycelium transfer
    #1003490 - 10/29/02 04:45 PM (14 years, 2 months ago)


  Ok, well I have to start new jars here pretty soon, and I want to clone my substrain of koh-samui. I have one PF style jar left that is not cased. I plan on mixing some of that colonized subsrate with water, and innoculating more jars with the mixture. I really dont have anyones personal opinions on how well this method works, I have just read about it. I am not sure how well the oven tech works with stuff like mycelium transfer. I know that it requires more sterile procedure that simply innoculating. Wil the oven tech work well??

  I have a dynamic sporanator I won last year, so I will use that. I am going to fill it with water, then boil it along with my syringes, scalpel, tools ect. I will mix some broken glass in with the water to break up the mycelium(how well does glass work for breaking up the chunks?)

How clean does my kitchen emviroment have to be? I will wear a mask and sterile gloves for this. I will remove the sterile sporanator with the water and glass, with sterile tongs, and set on oven rack. Wipe colonized jar with lysol, then set on oven rack.  Flame sterilize spoon,then set on oven rack. Open jar, smash on oven till it comes out. Set on oven rack. Loosen lid for sporinator. Grab spoon, ripp cake in half, grab chunk of colonized substrate with spoon. Quickly open lid and drop in, then close lid.

Ok, do you think those are carful enough procedures?? Thanks a lot for any advice:)

PS: spray the whole area with lysol, but thats a given.


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Offlinesocratesmind
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Re: PAranoid:Oven tech mycelium transfer [Re: Glacius]
    #1003494 - 10/29/02 04:47 PM (14 years, 2 months ago)

I've read of people doing agar work in open air in a bathroom with alot of lysol and cleaning surfaces with bleach solution and being really still and no current moving around.


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Offlinetsimehc
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Re: PAranoid:Oven tech mycelium transfer [Re: socratesmind]
    #1004479 - 10/29/02 10:57 PM (14 years, 2 months ago)

hah...just the oven if you dont have one of those expensive hoods


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Offlinehsalf
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Re: PAranoid:Oven tech mycelium transfer [Re: Glacius]
    #1004541 - 10/29/02 11:13 PM (14 years, 2 months ago)

I'd be worried that the heat might affect the mucellium in a negative way. Why no a cheap glove box? If no a flowhood.
Tell you what was it 102*F-106*F that mycellium dies? Your lowest setting in the oven about 250*F. Maybe 200*F.
Now with the oven open it won't be quite that hot but...
...get your thermometer. Unfortunatelly I'm guessing that it won't be good.
But let me know I never used the oven tek 4 anything...
...but mushroom pizza.


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OfflineFred Garvin
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Re: PAranoid:Oven tech mycelium transfer [Re: hsalf]
    #1004567 - 10/29/02 11:25 PM (14 years, 2 months ago)

I have used the oven tek with no problems. If you are doing a lot of jars, do them in groups and work quickly.



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Offlinedjneutron
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Re: PAranoid:Oven tech mycelium transfer [Re: Fred Garvin]
    #1004654 - 10/30/02 12:01 AM (14 years, 2 months ago)

just my 2 cents...u didn't say exactly in what way u r using the oven tek...what u need to do is close the lid on your oven, and crank it ALL the way up, usually 500 degrees or so, leave it that way for 40 mins to an hour, that will kill all contams inside oven...turn oven off, let cool for an hour, then open lid and work inside oven on botton or oven tray...just work fast and everything will be about as sterile as u can get it....when i first started this i always used the oven tek and never had any probs.... i now use a glove box, but the oven will work...just make sure u kill all contams before hand by heating it high for a while....

Hope that helps :smile:


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Offlinetsimehc
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Re: PAranoid:Oven tech mycelium transfer [Re: hsalf]
    #1004658 - 10/30/02 12:03 AM (14 years, 2 months ago)

see.. your not putting your stuff inside the oven... just extended out on a rack.. when i innoculated my pint jars with 1 cup grass seeds.... i had to do a bunch of stuff and the oven was really hot... well.. eventually i got my sytringe full of mycellium via honey water and injected... i was worried that the surface of the glass was hot and thought it would sterilize the mycellium... but after 48 hours of incubating around at 80 i have small spots and next day.. large rapid growth....

the oven works well.. i think i dropped one jar inside the oven and i had to get it.. its because of the rack.. its not flat you know.. so it rolled and some seeds fell out... i just recapped... opened a little and innoculated if nothing happened and so far i dont see any contams...

smell of burning seeds suck... dont spill any seeds in oven... hehehe.. ;]


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OfflineDERRAYLD
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Re: PAranoid:Oven tech mycelium transfer [Re: tsimehc]
    #1004797 - 10/30/02 12:50 AM (14 years, 2 months ago)

The oven never heats enough to harm the mycelium.
I put everything into my oven and work that way, half in and half out.
And so far I`ve had 100% success.


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Offlinehsalf
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Re: PAranoid:Oven tech mycelium transfer [Re: tsimehc]
    #1004826 - 10/30/02 12:58 AM (14 years, 2 months ago)

ty & garvin 2
still glovebox & flowhood is what i'd do


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OfflineGlacius
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Re: PAranoid:Oven tech mycelium transfer [Re: Glacius]
    #1004936 - 10/30/02 01:33 AM (14 years, 2 months ago)

Thanks for the input:)


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Amazon Shop for: ½ Pint Jars, Agar

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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