Home | Community | Message Board


Gaiana.nl
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: MushCult - Live Mushroom Cultures Edible and Medicinal Mushroom Cultures on Agar   Amazon ½ Pint Jars, Agar

Jump to first unread post. Pages: 1
Anonymous

liquid culture
    #905073 - 09/24/02 05:37 PM (16 years, 2 months ago)

This is what i over heard some guy say at a rest area : 4 grams of maltose dextrose from a Mr. Beer brew kit was added to 100ml of water, sterilized, and poured into a 1/2 pint jar that had a layer of fully colonized agar at the bottom, and agitated, then 1 ml of h2o2 was added. Then he said : its going on day four and there is no sign of any growth into the solution, even when looking very close at the colonized parts of the agar, it has made not even a hint of progress. He was wondering if something went wrong (not enough sugar, or maybe some type of preservative in it, or is the h2o2 inhibiting growth?) He was under the impression it would only take a week, especially with a whole colonized plate. Is this normal?


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblemickey_rourke
Illegal Smile
Registered: 07/18/02
Posts: 3,333
Loc: Playa del Carmen
Re: liquid culture [Re: ]
    #905110 - 09/24/02 05:54 PM (16 years, 2 months ago)

It shouldn't take too long, you are correct. I suspect that either your mix was wrong, or you may have contamination. I unfortunately do not have experience using pure dextrose. I add about 1/2 teaspoon of light corn syrup to 100ml of water and it works well.


--------------------
"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Shop: MushCult - Live Mushroom Cultures Edible and Medicinal Mushroom Cultures on Agar   Amazon ½ Pint Jars, Agar

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* liquid culture using h2o2 baja 3,836 14 03/10/17 12:00 AM
by 3B3B4E
* Liquid Culture Difficulties
( 1 2 all )
FungusWarrior 4,923 20 01/01/05 11:27 AM
by MushroomFriend
* Spores, G2G, Liquid Culture, Cloning & Colonization Time.
( 1 2 all )
agar 32,268 38 12/05/11 11:40 PM
by soicyboy121
* Liquid Culture Challenges Leimana 6,927 4 06/01/05 01:33 AM
by Corporal Kielbasa
* 1st Liquid Culture, arrrgh! Seril 5,968 10 02/28/06 10:35 PM
by Limelight_Liver
* Liquid Culture Recipes MrMaddHatter 8,580 13 03/29/05 06:53 AM
by Sbealzebub
* i can get liquid cultures, but not grain laughingbuddha 1,826 8 03/08/05 02:02 PM
by laughingbuddha
* how to use cardboard cultures to inoculate grain jars and how to use a liquid culture to inoculate lordoftheshroomz 4,869 12 05/08/05 05:55 PM
by lordoftheshroomz

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Roadkill, Magash, Shroomism, george castanza, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
425 topic views. 31 members, 61 guests and 18 web crawlers are browsing this forum.
[ Print Topic ]
Search this thread:
Vesp.co, Inc.
Please support our sponsors.

Copyright 1997-2018 Mind Media. Some rights reserved.

Generated in 0.027 seconds spending 0.009 seconds on 19 queries.