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Anonymous
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 liquid culture 
#905073 - 09/24/02 05:37 PM (15 years, 8 months ago) |
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This is what i over heard some guy say at a rest area : 4 grams of maltose dextrose from a Mr. Beer brew kit was added to 100ml of water, sterilized, and poured into a 1/2 pint jar that had a layer of fully colonized agar at the bottom, and agitated, then 1 ml of h2o2 was added. Then he said : its going on day four and there is no sign of any growth into the solution, even when looking very close at the colonized parts of the agar, it has made not even a hint of progress. He was wondering if something went wrong (not enough sugar, or maybe some type of preservative in it, or is the h2o2 inhibiting growth?) He was under the impression it would only take a week, especially with a whole colonized plate. Is this normal?
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 mickey_rourke
Illegal Smile
Registered: 07/18/02
Posts: 3,333
Loc: Playa del Carmen
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Re: liquid culture [Re: ]
#905110 - 09/24/02 05:54 PM (15 years, 8 months ago) |
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It shouldn't take too long, you are correct. I suspect that either your mix was wrong, or you may have contamination. I unfortunately do not have experience using pure dextrose. I add about 1/2 teaspoon of light corn syrup to 100ml of water and it works well.
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"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five
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