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InvisibleTripsAreForKids
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Registered: 04/23/01
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Isolation, and Re-isolation, and Re-isolation...
    #898899 - 09/22/02 10:15 AM (15 years, 9 months ago)

Im not sure if anyone asked this specific question before so don't yell at me..  :grin:

Anyways, I've read that transfering mycelium over and over on agar reduces its vigor on other substrates, but what if I wanted to use the same substrate that's mixed with the agar to produce the fruits?

Like making brown rice flour agar, and re-isolating the rhizomorphic mycelium over and over, and over again.  It would grow very well on it wouldn't it?  Then if i transfered it over to a whole grain brown rice jar, wouldn't it do alot better than the original mycelium?


More information please? 


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Everything posted above was out of fun, none should be taken serious. I am currently under the influence so take that into consideration.


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OfflineDinoMyc
Ipsa scientiapotestas est
Registered: 11/14/99
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Last seen: 12 years, 1 month
Re: Isolation, and Re-isolation, and Re-isolation... [Re: TripsAreForKids]
    #899093 - 09/22/02 12:50 PM (15 years, 9 months ago)

People have brought it up before, yes, but it is a hard topic to search for, so well cut a little slack :smile:

Stament's personal opinion on the subject is that it is beneficial to add a small amount of the final substrate to the agar.  There is less delay between substrates (transfer to grain, for example) if this is done.

Why would you bother with BRF?

Why would you expand the dish repeatedly? After a too many such expansions, senescence would become a concern.

edit, stupid spelling mistake.


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If I made affront, I apologize.
If I made affirmation, I apologize.
I merely came to listen, came to say.


Edited by DinoMyc (09/22/02 08:38 PM)


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InvisibleTripsAreForKids
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Registered: 04/23/01
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Re: Isolation, and Re-isolation, and Re-isolation... [Re: DinoMyc]
    #899885 - 09/22/02 08:02 PM (15 years, 9 months ago)

In reply to:

Why would you bother with BRF?




Cause i have like 4 lbs from the first time i grew, and i've yet to use any of it.


I read the thread is this forum called "Agar Isolations" and someone posted that if you keep isolating rhizomorphic portions and growing it on agar over and over, it would become accustomed to the media and grow faster and faster every time. So I figured if you do it with the substrate you plan to use in the very end, it would colonize faster, thus fighting contams better.


In reply to:

Why would you expand the dish repeatedly? After a too many such expansions, senesces would become a concern.




Whats senesces?


--------------------
Everything posted above was out of fun, none should be taken serious. I am currently under the influence so take that into consideration.


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OfflineDinoMyc
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Registered: 11/14/99
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Re: Isolation, and Re-isolation, and Re-isolation... [Re: TripsAreForKids]
    #899963 - 09/22/02 08:40 PM (15 years, 9 months ago)

Bake bread, make fried foods..

get grain instead. BRF is a waste of agar/effort.
It is true that as you select the sectors of mycelium which appear to express desirable traits, you can isolate a good culture, however you cannot do this indefinately. Senescence
(Spelling error in last post, sorry) is essentially just being 'old'.
You seem apropriately interested in fungi, you would be very well served with The Mushroom Cultivator and Growing Gormet and Medicinal Mushrooms, by Staments and Chilton.
Enjoy, good luck.
(edit, still spelling the word wrong)


--------------------
If I made affront, I apologize.
If I made affirmation, I apologize.
I merely came to listen, came to say.


Edited by DinoMyc (09/23/02 03:38 PM)


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Invisiblezeta
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Registered: 05/25/02
Posts: 3,972
Re: Isolation, and Re-isolation, and Re-isolation... [Re: DinoMyc]
    #900724 - 09/23/02 02:05 AM (15 years, 9 months ago)

*senescence


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Offlineblackout
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Re: Isolation, and Re-isolation, and Re-isolation... [Re: zeta]
    #901345 - 09/23/02 10:55 AM (15 years, 9 months ago)

i have read that a cloned shroom will not grow as well on a different substrate than what it was originally grown on. has anybody experienced this? it seems to make sense. would it be worthwhile, if starting with a multispore "honey water", to innoculate lots of jars with a "honey water" jar actually made from the substrate you plan on using. e.g. a liquid broth made from rye flour? or starting with a rice broth if growing sclerotia


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InvisibleTripsAreForKids
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Registered: 04/23/01
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Re: Isolation, and Re-isolation, and Re-isolation... [Re: DinoMyc]
    #901415 - 09/23/02 11:34 AM (15 years, 9 months ago)

I've always wanted that book, but I've just never gotten around to getting a hold of it. Next paycheck I'll goto the store and get them to order it by isbn... Both of them... Thanks for the info.


--------------------
Everything posted above was out of fun, none should be taken serious. I am currently under the influence so take that into consideration.


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InvisibleZen Peddler
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Registered: 06/18/01
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Re: Isolation, and Re-isolation, and Re-isolation... [Re: blackout]
    #913329 - 09/27/02 10:12 PM (15 years, 9 months ago)

If your refering to my post, then i didnt mean that. What i meant was that if you reisolate an original isolate for an extended period - or even cultivate a spore-race from a specific genetic stock it will grow towards being reliant on the one type of substrate and will perform selectively on that substrate, and crap on others.
BRF is shit - Millet is the best substrate!!!
Lastly, as already stated scenescing will be an issue (spelt wrong i know!) for the same reasons - in that the reisolation ages the clone to a point where the mycelia loses its original rhizomorphic vigour


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