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Mushrooms, Mycology and Psychedelics > Gourmet and Medicinal Mushrooms
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Cloning store bought portabellas
    #797364 -

somebody bought some mushrooms and put them in the frige. i was making some eggs with mushroom, and when i pulled one out, there was vigorous myceleal growth at the trunk of all of them. ot was fanning out at least 1/8th inch. i want to clone them. there was no species listed, but i would assume there portabellas. what agar mix is recommended. I have some clean plates of a yeast/malt/brf mix. would this be alright? or maybe a dextrose mix?


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Re: Cloning store bought portabellas [Re: Shaw]
    #798484 -

Stamets recommended PDYA, OMYA or DFA...and said PDYA worked best for him.

-psyco


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It may look like a button mushroom right now, but wait until you see how it grows!

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Re: Cloning store bought portabellas [Re: psyconaut]
    #802567 -

hm... now that you write about agar, it is the right time to ask.

Agar is something that i cant find here [in greece], probably because i dont know what it is exactly, so i dont know where to search for it.

1) what kind of stores have it?
2) what is the form of it? [like flour, like grains, like liquid...like what?]

THANX!

Kykeon
athens, greece


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The living ghost of Kykeon

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Re: Cloning store bought portabellas [Re: kykeon]
    #802674 -

1> try eastern food store like Thai or Chinese
2> it,s like a powder-flake thing

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Re: Cloning store bought portabellas [Re: Shaw]
    #803229 -

i believe bluhoney stated that you can find it in health food stores as a product to harden fingernails. its not as good as agar made specificly for lab work, but will suffice in a pinch. i havent tried looking yet, but blu knows her stuff


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ISO: Orissa, Malabar, z strain

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Re: Cloning store bought portabellas [Re: ChromeCrow]
    #803488 -

Here's the pop on agar....

Agar is a polysaccharide found in the cell walls of some red algae and is unusual in containing sulfated galactose monomers (gelling agents). It requires nothing but extraction from red algae and purification to become agar.? Agar added to liquids simply gels them into a convenient solid form. It is used as a gelatin substitute for Jell-O? like desserts. Agar will form gels at approximately 88F(35C) but once formed does not melt below 136F(85C).

It's used a lot in food preparation where folk don't want to use gelatin (real gelatin is made from animal bones, therefore not veggie friendly). For example, it can be used as a setting agent for jams and jellies.

Incidentally, it's really called agar agar when buying it in its purest form. If you can find an asian food store, you might also ask for it as "Kanten", it's Japanese name.

You'll probably find the powdered, rather than flaked, form easier to work with.

-psyco


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It may look like a button mushroom right now, but wait until you see how it grows!

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Re: Cloning store bought portabellas [Re: Shaw]
    #810522 -

i have allways wanted to clone from a store-bought, but alas i have not had good success in agar, probably kuz my kitchen is contaminated. i use honey-water to clone.

first soak your specimen in hydrogen peroxide straight out of the bottle for one minute. then cut out your desired portion. i often use a rather large chunk, but not necessary. place this into a sterilized blender with about a quarter of a cup of steril honey-water. blend until all chopped up. some authors recomend not over-blending.

pour the resultant foamy mixture into the quart jar that was used to sterilize the honey water in the first place. it's okay if there's an other cup or so of honey-water in the jar. cap. wait a few days or untill you see growth. sometimes helps to reblend. this is where an Oster blender works great, since the pitcher base has a mason jar thread on it, so one does not need to transfer to a pitcher and back, risking contamnation.

typicly in 5 days, you will have licuid inoculant ready to suck up into a syringe or spoon onto a suitably prepared spawn substrate.

there are threads on how to honey-water clone on this site.

Edited by tchyted (08/11/02 08:40 AM)

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Mushrooms, Mycology and Psychedelics > Gourmet and Medicinal Mushrooms
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