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Re: Cloning store bought agaricus? [Re: zeronio]
    #1513801 -

How does ggmm describe the mycelium?


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Re: Cloning store bought agaricus? [Re: Paid]
    #1513875 -

Quote:
Longitudianlly linear, thickening with age to form a dense cottony white mycellial mat, becoming mottled with brown discoloration in age.




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Re: Cloning store bought agaricus? [Re: zeronio]
    #1513876 -

It also says that wild strains can be easily brought into culture. :grin: 

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Re: Cloning store bought agaricus? [Re: zeronio]
    #1513885 -

lol, i just rechecked mine, and 2 had some kind of pin mould :smirk:
easy? lol


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Re: Cloning store bought agaricus? [Re: canid]
    #1514205 -

I did the isolation on antibiotic oatmeal agar. The contams were mostly Trichoderma and Botyritis, not bacteria. I think Z- is right, I think they were older rehydrated fruits rather than fresh ones.....What selective media suggestions do you have?


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Listen! Do you smell something?

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Re: Cloning store bought agaricus? [Re: r05c03]
    #1514964 -

r05c03: if your cultures are free of bacteria you might try multiple point transfers to ACmA. this should retard the growth of both the contaminant molds and the target mycelium. as soon as you notice mold sporulation in the plate carefuly transfer from non sporulating growth onto several plates untill you get a plate free of sporulating molds.

just a suggestion.


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Attn PWN hunters: If you should come across a bluing Psilocybe matching P. pellicolusa please smell it.
If you detect a scent reminiscent of Anethole (anise) please preserve a specimen or two for study and please PM me.

Edited by concretefeet (05/02/03 01:32 PM)

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Re: Cloning store bought agaricus? [Re: Paid]
    #5373750 -

Paid said:
Quote:
Can agaricus be fruited off of cased grains?




TMC says :
This species fruits well on rye grain covered with an unsterilised peat based casing layer.


Spawn Medium Rye grain buffered with calcium carbonate and/ or calcium sulfate.





Is there a tek on a substrate and casing mix thats good for A.bisporus that anyone knows of? I am cultivating from a store bought pre-innocced kit and I took some prints, I want to try to cultivate some from my prints next. I'm searching and not coming up with anything specific.thanks


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Edited by TheWay (03/07/06 10:30 AM)

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Re: Cloning store bought agaricus? [Re: TheWay]
    #5373942 -

um..... 3 years late


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buh

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