Snapple/Karo Liquid culture guide

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galadar

Registered: 05/09/07
Posts: 981
Loc: in a monotub!
Last seen: 4 years, 10 months

Snapple/Karo Liquid culture guide

1
#7863201 - 01/11/08 01:14 AM (18 years, 8 months ago)

OK guys, heres is a great way to use all them damn snapple bottles lying around. Snapple bottles are just pint jars with an obvious 1 piece lid. This guide will help cover preparation of the bottles as well as a simple and effective liquid culture technique.
The prepared snapple jars are also a GREAT grain jar.

ALL PICTURES ARE OF LARS IN NORWAY

Materials Needed
Snapple Bottle(standard 1pint glass bottle)
Hammer for punching lid hole and safely breaking glass bits
Wood Block of any size It helps with hammering a nail into the lid
Small nail (just tiny enough to make a tiny hole for the larger lid)
Larger Nail No Larger than a bic pen's thickness
Broken Glass Anything crystal clear, or any agitator can work
Karo Light Corn Syrup Any light corn syrup should work but Karo is the #1 brand, this should cost you $2-3
Polyfill Rip up that dumb teddy bear or pillow
Micro pore tape Optional
Empty Syringe Optional
Scissor Optional

Procedures

1. Wash out the peach iced tea from your snapple bottle and rinse well use soap and water. and remove the label.

2. While that is drying, Take your wood block, snapple lid and small nail. Have the snapple fact facing upwards and hold a nail towards the edge where the rubber seal ring is, don't get too close to it, leave it a few millimeters away. This helps in extracting ready liquid culture much easier than if the hole was in the middle.
(The reason we use a small nail first and then the bigger nail is so that the metal lid doesn't warp or bend;it is thin and small.)
Hammer your nail straight into the lid. and pull out.

3. Repeat with the Larger nail and pull out.

Try to flatten out the spiky bits of metal if you can.

4. Grab some polyfill the size of a small cotton ball. and pinch it up. Then from the top of the lid push it a bit in and from under, grab it and pull about 2 centimeters in till snug.

You can optionally snip off the excess fluff from the inside section, But keep the fluffy outside mess the way it is.when you do that make sure to rub your finger over it to get the tiny bits hanging off so they don't fall in later.

4. Take an old drinking glass or shot glass and wash it out nicely. put it into a plastic bag and hit it once or twice till you have dime to nickel size pieces. gram about 5-10 of em and rinse em of glass dust and place inside snapple bottle.

5. Measure out 200 milliliters of water into the bottle. or just fill about less than half way up. then cover lightly with foil so no dust falls in.

6. Here is what we need, Karo light corn syrup. it comes in a small bottle about the size of a snapple bottle.

Now just gently wash out a syringe and take off the needle.
Withdraw 10mL of corn syrup for your 200mL of water. Or just 2Teaspoons.

Lift foil and squirt into bottom of snapple bottle(try not to get any on the sides or lip)
Place Cap on bottle and voila! simple 5% LC solution.

This is how a bottle should look, minus everything else ha ha

7. Cover top with foil and pressure cook for 45 mins at 15psi.

8. When jar is cooled, wrap 2 layers of micro pore tape around the edges just to be safe. this is totally unnecessary though but why the hell not!

9. Use your favorite spore syringe or LC syringe and favorite glove box or flowhood and poke the needle STRAIGHT through the polyfill. none of that push it to the side and inject bullcrap.
Inject however much spores you want. i injected my last 4 or 5 ml into this. Pull out, and incubate at 75-80 degrees and you'll be golden.

10. When incubating, you'll start seeing white wispy puffy stuff near the bottom within a week. this is the point where you start stirring.
To do so, Hold the top of the jar and swirl the bottom around. just do not get the solution to splash around. a gentle swirl with the glass bits should handle breaking up the mycelium greatly.

And there you have it. a Simple snapple/karo guide that has worked extremely well for me over the ages.

leave me plenty of comments and such guys

Edited by galadar (01/19/08 03:42 AM)

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