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Offlinegaladar


Registered: 05/09/07
Posts: 981
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Last seen: 2 years, 2 months
Snapple/Karo Liquid culture guide
    #7863201 - 01/11/08 01:14 AM (16 years, 21 days ago)

OK guys, heres is a great way to use all them damn snapple bottles lying around. Snapple bottles are just pint jars with an obvious 1 piece lid. This guide will help cover preparation of the bottles as well as a simple and effective liquid culture technique.
The prepared snapple jars are also a GREAT grain jar.

ALL PICTURES ARE OF LARS IN NORWAY

Materials Needed
Snapple Bottle(standard 1pint glass bottle)
Hammer for punching lid hole and safely breaking glass bits
Wood Block of any size It helps with hammering a nail into the lid
Small nail (just tiny enough to make a tiny hole for the larger lid)
Larger Nail No Larger than a bic pen's thickness
Broken Glass Anything crystal clear, or any agitator can work
Karo Light Corn Syrup Any light corn syrup should work but Karo is the #1 brand, this should cost you $2-3
Polyfill Rip up that dumb teddy bear or pillow
Micro pore tape Optional
Empty Syringe Optional
Scissor Optional




Procedures

1. Wash out the peach iced tea from your snapple bottle and rinse well use soap and water. and remove the label.


2. While that is drying, Take your wood block, snapple lid and small nail. Have the snapple fact facing upwards and hold a nail towards the edge where the rubber seal ring is, don't get too close to it, leave it a few millimeters away. This helps in extracting ready liquid culture much easier than if the hole was in the middle.
(The reason we use a small nail first and then the bigger nail is so that the metal lid doesn't warp or bend;it is thin and small.)
Hammer your nail straight into the lid. and pull out.



3. Repeat with the Larger nail and pull out.

Try to flatten out the spiky bits of metal if you can.


4. Grab some polyfill the size of a small cotton ball. and pinch it up. Then from the top of the lid push it a bit in and from under, grab it and pull about 2 centimeters in till snug.


You can optionally snip off the excess fluff from the inside section, But keep the fluffy outside mess the way it is.when you do that make sure to rub your finger over it to get the tiny bits hanging off so they don't fall in later.


4. Take an old drinking glass or shot glass and wash it out nicely. put it into a plastic bag and hit it once or twice till you have dime to nickel size pieces. gram about 5-10 of em and rinse em of glass dust and place inside snapple bottle.


5. Measure out 200 milliliters of water into the bottle. or just fill about less than half way up. then cover lightly with foil so no dust falls in.


6. Here is what we need, Karo light corn syrup. it comes in a small bottle about the size of a snapple bottle.

Now just gently wash out a syringe and take off the needle.
Withdraw 10mL of corn syrup for your 200mL of water. Or just 2Teaspoons.

Lift foil and squirt into bottom of snapple bottle(try not to get any on the sides or lip)
Place Cap on bottle and voila! simple 5% LC solution.

This is how a bottle should look, minus everything else ha ha

7. Cover top with foil and pressure cook for 45 mins at 15psi.

8. When jar is cooled, wrap 2 layers of micro pore tape around the edges just to be safe. this is totally unnecessary though but why the hell not!



9. Use your favorite spore syringe or LC syringe and favorite glove box or flowhood and poke the needle STRAIGHT through the polyfill. none of that push it to the side and inject bullcrap.
Inject however much spores you want. i injected my last 4 or 5 ml into this. Pull out, and incubate at 75-80 degrees and you'll be golden.


10. When incubating, you'll start seeing white wispy puffy stuff near the bottom within a week. this is the point where you start stirring.
To do so, Hold the top of the jar and swirl the bottom around. just do not get the solution to splash around. a gentle swirl with the glass bits should handle breaking up the mycelium greatly.


And there you have it. a Simple snapple/karo guide that has worked extremely well for me over the ages.

leave me plenty of comments and such guys


Edited by galadar (01/19/08 03:42 AM)


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Offlinebudzeno
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Registered: 10/14/07
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Re: Snapple/Karo Liquid culture guide [Re: galadar]
    #7863238 - 01/11/08 01:23 AM (16 years, 21 days ago)

A + Idea, No more baby bottles for me!


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Offlinenavyseals101
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Re: Snapple/Karo Liquid culture guide [Re: budzeno]
    #7863351 - 01/11/08 02:05 AM (16 years, 21 days ago)

I assume you just tilt the jar to get the syringe in the liquid?


--------------------
"Ten thousand flowers in spring
the moon in autumn,
a cool breeze in summer,
snow in winter.
If your mind isn't clouded by unnecessary things,
this is the best season of your life."
            -wumen


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Offlinegaladar


Registered: 05/09/07
Posts: 981
Loc: in a monotub!
Last seen: 2 years, 2 months
Re: Snapple/Karo Liquid culture guide [Re: navyseals101]
    #7863357 - 01/11/08 02:07 AM (16 years, 21 days ago)

Quote:

navyseals101 said:
I assume you just tilt the jar to get the syringe in the liquid?



yes and that was the main reason to make a hole towards the side of the lid. and when extracting it out with a syringe, all needle injections should go through to polyfil as its self sealing and wont go anywhere as long as you have a pointy needle.


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Invisible04281969
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Re: Snapple/Karo Liquid culture guide [Re: galadar]
    #7863823 - 01/11/08 06:55 AM (16 years, 21 days ago)

Sounds a little tall (deep), but a handy idea.


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OfflineSupplier
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Re: Snapple/Karo Liquid culture guide [Re: 04281969]
    #7863847 - 01/11/08 07:10 AM (16 years, 21 days ago)

and it stays liquid inside the snapple bottle?


--------------------
:mushroomgrow: :pinkshroom: :mushroomgrow: :pinkshroom: :mushroomgrow:


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Invisibleblood4blood
Calmer Than You Are


Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: Snapple/Karo Liquid culture guide [Re: Supplier]
    #7863869 - 01/11/08 07:23 AM (16 years, 21 days ago)

dont you think getting the poly wet while you extract lc will be a problem for contams?


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Offlinegaladar


Registered: 05/09/07
Posts: 981
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Last seen: 2 years, 2 months
Re: Snapple/Karo Liquid culture guide [Re: blood4blood]
    #7863932 - 01/11/08 07:57 AM (16 years, 21 days ago)

well what you do is lean the jar till liquid comes close to the hole. and by having the hole on the side, it will make it much more easier to extract.


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Offlinegaladar


Registered: 05/09/07
Posts: 981
Loc: in a monotub!
Last seen: 2 years, 2 months
Re: Snapple/Karo Liquid culture guide [Re: Supplier]
    #7863935 - 01/11/08 08:00 AM (16 years, 21 days ago)

Quote:

Supplier said:
and it stays liquid inside the snapple bottle?



stays liquid? what are you talking about.


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Invisibleblood4blood
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Registered: 04/25/07
Posts: 6,029
Loc: The Valley
Re: Snapple/Karo Liquid culture guide [Re: galadar]
    #7863938 - 01/11/08 08:01 AM (16 years, 21 days ago)

can anybody take lc's by themself? i was just wondering this because i tryed to pull one by myself the other day and no way was that happening... i had to have my wife help me.

im thinking og making some little device where you can do this on your own.


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Invisible04281969
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Re: Snapple/Karo Liquid culture guide [Re: blood4blood]
    #7863965 - 01/11/08 08:26 AM (16 years, 21 days ago)

I'm sure it would stay liquid if you used it quickly enough, and kept it "shook".
Maybe a marble or small quartz crystals in the bottom would be an alternative to broken glass.


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Invisible04281969
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Re: Snapple/Karo Liquid culture guide [Re: blood4blood]
    #7863970 - 01/11/08 08:30 AM (16 years, 21 days ago)

Quote:

blood4blood said:
dont you think getting the poly wet while you extract lc will be a problem for contams?




How about putting the polyfill into the pinky of a nitrile glove first, then putting it through the hole?


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Offlinegaladar


Registered: 05/09/07
Posts: 981
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Last seen: 2 years, 2 months
Re: Snapple/Karo Liquid culture guide [Re: 04281969]
    #7863976 - 01/11/08 08:33 AM (16 years, 21 days ago)

broken glass is most abundant haha.
plus it shreds your mycelium into tiny bits very effectively.
I also like a once a day 5-10 second shake to aerate and break myc up.


Edited by galadar (01/11/08 08:46 AM)


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Offlinegaladar


Registered: 05/09/07
Posts: 981
Loc: in a monotub!
Last seen: 2 years, 2 months
Re: Snapple/Karo Liquid culture guide [Re: 04281969]
    #7864728 - 01/11/08 12:06 PM (16 years, 21 days ago)

Quote:

04281969 said:
Quote:

blood4blood said:
dont you think getting the poly wet while you extract lc will be a problem for contams?




How about putting the polyfill into the pinky of a nitrile glove first, then putting it through the hole?



huh?
and im pretty sure if youre smart, you wont get the filter wet. or at least significantly wet. as in less thna a drop


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Invisible04281969
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Re: Snapple/Karo Liquid culture guide [Re: galadar]
    #7864743 - 01/11/08 12:10 PM (16 years, 21 days ago)

I can see even the smartest guy accidentally splashing the filter with a nice big drop from a sloshed "goik".


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OfflineIolaa
iolaa, not lolaa
Registered: 08/28/07
Posts: 335
Loc: Humco
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Re: Snapple/Karo Liquid culture guide [Re: 04281969]
    #7864760 - 01/11/08 12:14 PM (16 years, 21 days ago)

it's a good idea, however those expensive BossaNova acai juice bottles look way sweeter. also, i don't even bother to punch a hole in the lid, i just kinda slosh some LC into a jar in my clean glove box. haven't had many problems yet.


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Invisible04281969
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Re: Snapple/Karo Liquid culture guide [Re: Iolaa]
    #7869688 - 01/12/08 04:31 PM (16 years, 20 days ago)

I wonder if you could get a really short baby bottle to work. The nipple would seal itself, more or less.


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InvisibleSofaKingGood
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Re: Snapple/Karo Liquid culture guide [Re: 04281969]
    #7873810 - 01/13/08 03:16 PM (16 years, 19 days ago)

Yes you could do that. There is a Tek somewhere dealing with using baby bottles.


--------------------
[quote]I8thesh400m said:
he-has-as-have-i-chek-My-ratings:thumbup:
to-the-post-origionator-5-to-you
there-are-nice-people-but-be-smart
my-spacebar-is-broke:p [/quote]


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Offlineskattman1982
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Re: Snapple/Karo Liquid culture guide [Re: SofaKingGood]
    #7873993 - 01/13/08 03:54 PM (16 years, 19 days ago)

i think i will try this in a day or two.


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InvisibleBlutjager
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Re: Snapple/Karo Liquid culture guide [Re: SofaKingGood]
    #7877551 - 01/14/08 09:41 AM (16 years, 18 days ago)

Quote:

SofaKingGood said:
Yes you could do that. There is a Tek somewhere dealing with using baby bottles.




I have a tek using baby bottles in my sig


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Invisiblesmokedout420
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Re: Snapple/Karo Liquid culture guide [Re: Blutjager]
    #7926814 - 01/24/08 09:22 PM (16 years, 8 days ago)

just put 2 silicone ports an you a airport syringe for gas exchange



this would work on the snapple great

edit: not my picture


Edited by smokedout420 (01/25/08 07:48 AM)


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Offlinegrod31
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Re: Snapple/Karo Liquid culture guide [Re: Blutjager]
    #7927485 - 01/24/08 11:16 PM (16 years, 8 days ago)

*deleted* should have read the second page of the post.


--------------------
Back the tape up.  I need it again!
Let it roll!  Just as high as the
fucker can go!  And when it comes
to that fantastic note where the
rabbit bites its own head off, I
want you to  THROW THAT FUCKING
RADIO INTO THE TUB WITH ME!
    Not me.  It would blast you through
the wall stone dead in ten
seconds and they'd make me explain
VVVVV Free Myco Thread


Edited by grod31 (01/24/08 11:17 PM)


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Invisiblebmiles
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Re: Snapple/Karo Liquid culture guide [Re: Blutjager]
    #8032037 - 02/16/08 05:59 PM (15 years, 11 months ago)

i used this TEK, check out my grow log below.
hope it works out :wink:


--------------------
Never go with a hippy to a second location.


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Offlinegaladar


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Re: Snapple/Karo Liquid culture guide [Re: bmiles]
    #8036213 - 02/18/08 12:14 AM (15 years, 11 months ago)

good luck =]


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Invisiblebmiles
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Re: Snapple/Karo Liquid culture guide [Re: galadar]
    #8036309 - 02/18/08 12:46 AM (15 years, 11 months ago)

i already see small white things, but it might be polyfill, haha.


--------------------
Never go with a hippy to a second location.


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Offlinegaladar


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Re: Snapple/Karo Liquid culture guide [Re: bmiles]
    #8036804 - 02/18/08 06:47 AM (15 years, 11 months ago)

haha yea i try to make sure its free of loose strands.


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Offlinedoc34
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Re: Snapple/Karo Liquid culture guide [Re: galadar]
    #8036844 - 02/18/08 07:18 AM (15 years, 11 months ago)

You are not the only one who uses them thar Snapple jars, hahaha:thumbup:
I have been using them for years as an LC jar-Those Starbucks coffee bottles work great too!
I also add the Tyvek for added insurance.
Nice to know other people are doing the same:cool:

Great write-up too


Doc


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Offlinegaladar


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Re: Snapple/Karo Liquid culture guide [Re: doc34]
    #8036881 - 02/18/08 07:37 AM (15 years, 11 months ago)

yea those 10 ounce stubby bottles.

so whats your way of preparing them? please share.


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Offlinedoc34
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Re: Snapple/Karo Liquid culture guide [Re: galadar]
    #8039413 - 02/18/08 06:34 PM (15 years, 11 months ago)

I take a quart jar(for measuring purposes) and fill it up with water(600-700ml) and then  add 2-3 tablespoons
of "Light Karo Corn syrup", shake to mix, then use that to fill up the snapple jars-(I use the quarts for WBS/grains).
I then add a layer of Tyvek,
then the lid, which has a 1/4' inch hole in the top, with poly fill, cover with foil, PC for 20 minutes @ 15psi,
let cool inoculate and then  set them in a box on the top shelf of a closet(Heat rises). Once I see a good chunk
of mycelia growing(5-10 days) I then set the jars in the door of the fridge to slow down growth and to
allow for the jars to be agitated every time I open the door This way they will keep a little longer so  I can use
them as I need them.

Hope that was helpfull:thumbup:


Doc


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Invisiblebmiles
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Re: Snapple/Karo Liquid culture guide [Re: doc34]
    #8039442 - 02/18/08 06:39 PM (15 years, 11 months ago)

that was helpful sir.
do you think a lil bit of polyfill floating
in the solution could hurt anything? i doubt it...


--------------------
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Offlinegaladar


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Re: Snapple/Karo Liquid culture guide [Re: bmiles]
    #8039952 - 02/18/08 08:24 PM (15 years, 11 months ago)

not at all. right now i have one or two strands floating. its just non nutritious sterile fiber.


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Invisiblebmiles
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Re: Snapple/Karo Liquid culture guide [Re: galadar]
    #8040979 - 02/18/08 11:39 PM (15 years, 11 months ago)

good deal, didn't think it would make a difference.


--------------------
Never go with a hippy to a second location.


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