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Offlinemellojello
O FUCKBEANS
Registered: 05/14/02
Posts: 137
Last seen: 9 years, 5 months
best way to innoculate
    #724281 - 07/05/02 09:27 AM (14 years, 5 months ago)

what is better: glove box? or Oven tek?


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OfflineAbyss
Fist full of Mushrooms
Male

Registered: 06/23/02
Posts: 137
Loc: NorCal
Last seen: 1 year, 18 days
Re: best way to innoculate [Re: mellojello]
    #724288 - 07/05/02 09:33 AM (14 years, 5 months ago)

Well, Glove box is the best way but since alot of people don't have glove boxes but do have an oven I would say the oven is more widely used. I know a friend of a fiend who knows someone who has a cat who only uses the oven tek and has never had a prob.

Peace love & hair grease,

Abyss


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OfflineC12H17N204P
enthusiast

Registered: 05/20/02
Posts: 204
Loc: Minnesota, USA
Last seen: 13 years, 11 months
Re: best way to innoculate [Re: mellojello]
    #724313 - 07/05/02 09:49 AM (14 years, 5 months ago)

Glove box is by far better if you've got the time to put on together. You'll be able to do everything you need to do sterile in there.


--------------------
"My mind is full of dreams...
My eyes filled with fantasies
I hear what I see
And see what I hear
My senses are not dulled, I simply see clear"

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"My heart is broke, but I have some glue, help me inhale, and mend it with you." - R.I.P. KDB


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InvisibleBoppity604
Stranger
Registered: 10/19/01
Posts: 1,056
Re: best way to innoculate [Re: C12H17N204P]
    #724370 - 07/05/02 10:40 AM (14 years, 5 months ago)

regardless of what tek you use if you don't remain mindful of sterility for all items used in the tek you could risk contaminating your substrates. Of course...no precaution in the world will stop an infected/contaminated syringe from destroying your grow...learn about sterility and what things can lead to contamination. Unless you're in a lab you can't gaurantee 100% sterility but it's obvious those of us who are careful escape the grasp of "the mold."

Good luck.

Boppity


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Offlinegoatywoaty
THing of thing

Registered: 04/11/02
Posts: 263
Loc: caught in the storm of en...
Last seen: 13 years, 3 months
Re: best way to innoculate [Re: Boppity604]
    #725507 - 07/05/02 09:48 PM (14 years, 4 months ago)

but it's obvious those of us who are careful escape the grasp of "the mold."

not true! some dog i overheard used the oven tek and two of four myco bags turned out green moldish. made his lonely dog heart sad, but he was reassured when the other two bags turned out fine.



--------------------
[real.eyes.realize.real.lies]
the only reality is the one you make of it, for in the end it becomes what it always was.
______________________________
:::anything above this line is a lie:::



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Offlinetwisted
member

Registered: 06/15/02
Posts: 177
Loc: croatia
Last seen: 5 years, 6 months
Re: best way to innoculate [Re: goatywoaty]
    #725867 - 07/06/02 05:58 AM (14 years, 4 months ago)

well, shit sometimes just happens


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Anonymous

Re: best way to inoculate [Re: twisted]
    #3760028 - 02/10/05 03:06 AM (11 years, 9 months ago)

could any one post a link to the EXACT oven tek??
thanks...


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Invisibleagar
old hand
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Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
Trusted Cultivator
Re: best way to inoculate [Re: mellojello]
    #3760387 - 02/10/05 05:36 AM (11 years, 9 months ago)


PHOTO BY ANNO

Oven tek is BS. Just my 0.02 cents


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OfflineAnand
AutisticShroomer
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Registered: 11/05/02
Posts: 365
Loc: 30 miles From Amsterdam
Last seen: 9 days, 9 hours
Trusted Cultivator
Re: best way to inoculate [Re: agar]
    #3760429 - 02/10/05 05:53 AM (11 years, 9 months ago)

Use a GloveBox, it saves money and just costs about $10 to make



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InvisibleHolydiver
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Folding@home Statistics
Registered: 03/20/01
Posts: 5,155
Loc: The midnight sea
Trusted Cultivator
Re: best way to inoculate [Re: Anand]
    #3760633 - 02/10/05 08:10 AM (11 years, 9 months ago)

Actually I haven't used the glove box, oven tek, or even an alcohol flame for inoculations in a long time. No need for it really. Just be quick and ensure that you cover the needle with alcohol for each hole, and you can do inoculations in any environment.

Transfers and G2G is another thing, but inoculations can be done successfully in some pretty dirty conditions in my experience. It's also a lot easier to shake a syringe when you aren't trying to work in a cramped glove box.



--------------------
To find a place to live between the negatives and positives.


Edited by Diver (02/10/05 08:15 AM)


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