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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Shop: Kraken Kratom Red Vein Kratom   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag

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OfflinePhake_ld
enthusiast
Registered: 10/08/01
Posts: 230
Last seen: 22 years, 4 months
INNOCULATION
    #446610 - 11/04/01 12:14 PM (23 years, 1 month ago)

If I were inncoulating my jars in a glove box, will I need to sterilize the needle by burining the tip before I innoculate each jar?

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Offlinelemming
evil

Registered: 10/28/01
Posts: 107
Last seen: 19 years, 3 months
Re: INNOCULATION [Re: Phake_ld]
    #446615 - 11/04/01 12:22 PM (23 years, 1 month ago)

You don't NEED to do it but it's always a good idea to sterilize as much as possible


--------------------
As evil as a dwarf can get...

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Invisiblephrozendata
Carpal Tunnel

Folding@home Statistics
Registered: 04/23/00
Posts: 5,015
Re: INNOCULATION [Re: lemming]
    #446623 - 11/04/01 12:39 PM (23 years, 1 month ago)

Maby just rub it down with a alcohol soaked paper towel. I cant see why that wouldnt work. Thats all we do to the paper we print on and i'm sure we want that more sterile than the needle tip. The only problem is who knows what gets on the paper towel when you whipe. It would take time to prepare a 12 section paper towel with alcohol. I feel your pain, flaming takes awhile.


--------------------
"There's only one corner of the universe you can be certain of improving and that's your own self. So you have to begin there, not
outside, not on other people" - Aldous Huxley

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OfflinePhake_ld
enthusiast
Registered: 10/08/01
Posts: 230
Last seen: 22 years, 4 months
Re: INNOCULATION [Re: phrozendata]
    #446638 - 11/04/01 12:54 PM (23 years, 1 month ago)

Do you think using a few alchol pads before each innoculation will work out well?

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Invisiblepuscle
genius of love
Registered: 01/06/01
Posts: 4,539
Loc: NY
Re: INNOCULATION [Re: Phake_ld]
    #446679 - 11/04/01 01:36 PM (23 years, 1 month ago)

I set my syringes in a alchohol filled shot glass, before and between jars. Be sure to flick off excess alchohol. It's all the sterilization you need.

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OfflineMuffinBear
liek

Registered: 05/26/00
Posts: 584
Last seen: 14 years, 4 months
Re: INNOCULATION [Re: puscle]
    #447575 - 11/05/01 10:17 AM (23 years, 1 month ago)

usually just wipe the needle off with an alcohol soaked cotton ball between jars and innoculate in a glove box. never really had any contam probs

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Invisiblemrshrums
member
Registered: 08/04/01
Posts: 147
Re: INNOCULATION [Re: MuffinBear]
    #447581 - 11/05/01 10:25 AM (23 years, 1 month ago)

I got my syringes from PF and they have this v shaped cut in the tip of the needle. When I wipe with cotton ball/pad/paper towel or anything I always get this damn cloth or cotton or whatever sticking in that v. Any suggestions? Do you guys have the same type syringes? If not where do you get your syringes? Wal-mart didn't have any sitting out nor did I expect they'd have them but it didn't hurt to look.

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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