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Amazon Shop for: ½ Pint Jars, 10cc Syringe, Agar, Petri Dish

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OfflineThe_Mushroom_God
member
Registered: 04/04/02
Posts: 115
Last seen: 13 years, 11 months
Glove Box Question
    #622907 - 04/30/02 06:40 AM (14 years, 7 months ago)

Does any one know if this glove box tek woks well when making syrenge's or is it bull shit? If none of you have used it I would still like you'r opinion's, this tek stood out to me because of my lack of fund's!!!! Anyway here it is!!!!

Build the Cheapest "Glove" Box Possible

Items Required

Cardboard box (13"x15"x10")
Plastic wrap
Duct tape
Dish cloth
Disinfectant spray
Sharp knife or razor blade for cutting cardboard
Rubbing alcohol
No gloves

To raise the odds against contamination, a sterile work area is vital. This is especially true if you want to become independent of outside sources of sterile spore syringes. In a rush I threw together this glove box out of scrounged materials. It has worked so well that I haven't built my dream box yet. First find a clean cardboard box with the above dimensions as a minimum. It doesn't have to be new or pristine, just fairly new with no visible dust or dirt. Cut off the top opening flaps. Cut away the entire front of the box, leaving a 2 inch wide strip of cardboard at the top edge to attach the dish cloth to. Cover the top with plastic wrap and tape down the edges. Using duct tape, attach a dish cloth or towel across the front opening onto that 2 inch strip. The cloth should cover the entire opening of the front of the box like a curtain and you should be able to see into the box through the clear plastic wrap on top. That's it. To use the box, spray down your work surface with the disinfectant spray. Close off all drafts (windows, heater fans, etc). Spray outside of box lightly and saturate the dish cloth. Place bulky items into the box such as grain jars and agar plates. Spray disinfectant into the box until all of the air inside is displaced by the mist. Wash hands with rubbing alcohol and you are ready to work



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Shrooms are great, by the way DEA every thing I say is false so suck my dick!!!!!!!!!







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OfflineNumba9
Veteran tripper
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Registered: 05/24/01
Posts: 508
Loc: State of "Euphoria"
Last seen: 8 years, 8 months
Re: Glove Box Question [Re: The_Mushroom_God]
    #622992 - 04/30/02 10:19 AM (14 years, 7 months ago)

Some people will argue this, but I say ditch the whole Glove box idea..... Use the oven tek..... I`ve innoculated over a 100 jars with not one contamination..... I`ll never go back to a box.Turn your oven on to the lowest setting.. take out the other racks except for the bottom shelf and put your jars on a plate and do your innoculations in the oven. The warm air rising will keep any contams from dropping down around your work area. Of course you still want to flame your needles and cool with lysol or alcohol before entering the jar . Try your next syringe work this way... I think you`ll like the results and how easy it is to work on your oven racks. Good luck. Oven tek works well when making syringes from prints also.



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Remember..what the Door Mouse said..."Feed your Head".......... Jefferson Airplane


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InvisibleMushroom_Madness
enthusiast
Registered: 01/10/02
Posts: 261
Loc: Quaint New England Villag...
Re: Glove Box Question [Re: The_Mushroom_God]
    #623019 - 04/30/02 11:04 AM (14 years, 7 months ago)

the golve box is a good idea, but we have a tek that is Newbie Friendly, and you do not need to use a glove box. The tek will show you how to make up to ten, 10cc spore syringes using your Dynamic Spore-a-Nator? in under 45 seconds (this is after you had made it to procedure three).

This procedure should be followed under sterile conditions. Here is the link to the tek my friend. http://mushroom-madness.com/cultivation_techniques/syringe_tek.htm

I hope this helps!


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OfflineSeussA
Error: divide byzero

Folding@home Statistics
Registered: 04/27/01
Posts: 23,480
Loc: Caribbean
Last seen: 22 days, 15 hours
Re: Glove Box Question [Re: Numba9]
    #623027 - 04/30/02 11:15 AM (14 years, 7 months ago)

> Some people will argue this

I am one of them, but I prefer to relate my experience rather than argue:

My friend used the oven tek for a long time with jars (50+) and never had a single failure. As my friend got more experienced she started using agar transfers instead of multispore innoculations. Petri dish after petri dish contaminated... every single one. About this time Anno posted a picture showing smoke getting sucked into an oven. My friend had never considered the oven tek was the source of her problem. After a bit of testing, she verified for herself what Anno had posted. She built a simple glove box simliar to the one described in the above post. Since then she has not had a single petri dish contaminate.

If the oven tek works for you by all means continue using it. However, if somebody is having contamination problems and they are using the oven tek, prehaps they should ditch it and try a glove box instead.


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Just another spore in the wind.


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OfflineThe_Mushroom_God
member
Registered: 04/04/02
Posts: 115
Last seen: 13 years, 11 months
Re: Glove Box Question [Re: Seuss]
    #623328 - 04/30/02 04:51 PM (14 years, 7 months ago)

Thank you for you'r help, I think I might try a syrenge or two all of these ways to see what work's best for me as I am a nawbie!!!!!


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Shrooms are great, by the way DEA every thing I say is false so suck my dick!!!!!!!!!







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Offlinecujo7999
Space Monkey

Registered: 04/21/02
Posts: 180
Loc: USA; Home of "The Man"
Last seen: 11 years, 1 month
Re: Glove Box Question [Re: The_Mushroom_God]
    #623396 - 04/30/02 06:29 PM (14 years, 7 months ago)

That's the key contamination phase. So if there are alot of contams it is probably a problem with your box/oven. In my opinion a box has to be better, i would use alcohol to clean the box, it kills everything, just like the heat in the oven. The box does not use air circulation like an oven does so things are more settled. You have also sectioned of a smaller space to work with so you chances get better with the less space your trying to keep perfectly clean.

Both work but if you have problems with one then try the other.


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*This is all Fiction by the Way*


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Offlinebluepoo
journeyman
Registered: 03/27/02
Posts: 83
Loc: europe
Last seen: 14 years, 2 months
Re: Glove Box Question [Re: The_Mushroom_God]
    #623647 - 05/01/02 12:51 AM (14 years, 7 months ago)

they work ok not as good as a flow but i does its job
id use a plastic box and a glass top about $6 extra


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the above is what the litel leprecans told me to say and should be regarded as such
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Amazon Shop for: ½ Pint Jars, 10cc Syringe, Agar, Petri Dish

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